LOCUS       40924_9291        6745 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-Dasl1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 6745)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6745)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1789..2811
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     rep_origin      complement(4845..5433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5610..6398)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6618..6722)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccagcgg ccgcgaaata gaagacgaag acgagaggag gagagggtgc
      421 gagagggtgc gaaactgcgc ccttcagctc gctaattccg cccattacat tcagcgcaca
      481 cttggtggta catacatacc tacatacgta cccgtagttg tgcgaagcga gtcgagccag
      541 cggtcggatg tgccaatatg taccttgtag gtactcatga tagtcagcag gaagccgagg
      601 gatgatagca cgcacagcca gtctggtgtt tgtgctctgg attcagcccc tagattcact
      661 gaccaaaaga agcaagagaa gggaaaggga aaaggaagcc ctaaaaaaga gcagggttgt
      721 ccttcggcac caccccgaaa gcaacagcag tgatggacgg gactacgaca ctgtagtaca
      781 tacgccaacc caaagattcc gagttgcaat cattgccaca aaattgctcg actgtggcgg
      841 gagaggggaa agtctccgcg agggcatctc tgatgagtcc cgtgccacgc cgggtttctt
      901 gccttgaatc accacaaaat gtctcagcgc ggctcatcca tggccgcctt tttgggcgtt
      961 tcgtattact cttgggtttg actgtattat cggtcaagag ccaaaggcca ctcggctttt
     1021 ccgcccttgc ctctcttcca tttccccagg cgctgatagt atgatagttt cccctcattg
     1081 cagctttgga cgtcaatgcc aataactgta ttgtattgac aaatcatttg tccaaagacg
     1141 accgacacct ctgatatcac atacgagtag tagtacatac aatccccccc aaactccgcc
     1201 tttttggcgc gtgtgtgccg cgtagctcgt gcacaatgtc gggccgccgc cgccagcggc
     1261 cacttgctta gcaggaacct agtcagccgt ccttgtgtga ttaatactag tgctaggtat
     1321 agagagagga gagagagaga gagagagaga gaggggaaaa aaggtgaggt tgaagtgaga
     1381 aaaaaaaaaa aaaaaaaaaa tccaaccact gacggctgcc ggctctgcca cccccctccc
     1441 tccaccccag accacctgca cactcagcgc gcagcatcac ctaatcttgg ctcgccttcc
     1501 cgcagctcag gttgtttttt ttttctctct ccctcgtcga agccgccctt gttcccttat
     1561 ttatttccct ctccatcctt gtctgccttt ggtccatctg cccctttgtc tgcatctctt
     1621 ttgcacgcat cgccttatcg tcgtctcttt tttcactcac gggagcttga cgaagacctg
     1681 actcgtgagc ctcacctgct gatttctctc cccccctccc gaccggcttg acttttgttt
     1741 ctcctccagt accttatcgc gaagccggaa gaacctctta acctctagat gaaaaagcct
     1801 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     1861 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     1921 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     1981 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     2041 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     2101 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     2161 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     2221 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     2281 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     2341 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     2401 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     2461 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     2521 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     2581 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     2641 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     2701 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     2761 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga ataatgcatg
     2821 gctttcgtga ccgggcttca aacaatgatg tgcgatggtg tggttcccgg ttggcggagt
     2881 ctttgtctac tttggttgtc tgtcgcaggt cggtagaccg caaatgagca actgatggat
     2941 tgttgccagc gatactataa ttcacatgga tggtctttgc gatcagtagc tagtgagaga
     3001 gagagaacat ctatccacaa tgtcgagtgt ctattagaca tactccgaga ataaagtcaa
     3061 ctgtgtctgt gatctaaaga tcgattcggc agtcgagtag cgtataacaa ctccgagtac
     3121 cagcaaaagc acgtcgtgac aggagcaggc tttgccaact gcgcaacctt gcttgaatga
     3181 ggatacacgg ggtgcaacat ggctgtactg atccatcgca accaaaattt ctgtttatag
     3241 atcaagctgg tagattccaa ttactccacc tcttgcgctt ctccatgaca tgtaagtgca
     3301 cgtaggaaac catacccaaa ttgcctacag ctgcggagca tgagcctatg gcgatcagtc
     3361 tggtcatgtt aaccagcctg tgctctgacg ttaatgcaga atagaaagcc gcggttgcaa
     3421 tgcaaatgat gatgcctttg cagaaatggc ttgctcgctg actgatacca gtaacacctt
     3481 tgcttggccg tctagcgctg ttgattgtat tcatcacaac ctcgtctccc tcctttgggt
     3541 tgagctcttt ggatggcttt ccaaacgtta atagcactag tctagcttgg atccattgcc
     3601 tttgcgaggg ccaattcaaa ggctggcatc atcagcgaag acgaattcca ggcgatcgag
     3661 cgtggcttgc tccaagtgat ggaagaatgg aagcagggca ctttcgccat catgccgaat
     3721 gacgaagatg tgagagggca cactcgattc taatctcaaa cttctttcga ggctgacaca
     3781 atggaccaga ttcatacggc aaacgaacgt cgcctgggag aggtcatcgg caaggacatc
     3841 gcgggaaagc ttcacacagg tcgcagccgc aacgagcaag ttgtctgtga tatgcgcatg
     3901 tggcttcgcg atcggatccg cgaaatcgac agccaactcg ttgctttcct caaggtcatt
     3961 atcacacgtg ccgaatctga aatgtaagcc cagccgtacc tttccagcta aatgcccccc
     4021 cccccccccc ccccctctct tactgacgga caacctcagc gactacatca tgcccggata
     4081 cacccatctc cagcgcgctc agccagttcg gtggtctcaa tggctcatgt cctatgccgc
     4141 tgcgttcaag caggatctcg agcggttgcg tcaagtcttc gagagggtca acctcagtcc
     4201 acttggctgt ggcgccttgg ccggcaatcc attcggaatc gaccgagact caatagctgc
     4261 ggagcttggc ttcagcggaa tcaccttgaa ctccatgaat acttcggccg accgcgattt
     4321 cctcgtcgat ttcctgtcct ggaactccat cttcaccaac cacgtcagca ggtgggctga
     4381 ggacctcatc atctactcga cctctgagtt cagtttcgtc cgtctggccg acgcctattc
     4441 cacgggcagc tctctcatgc ccaacaaatt caacccggac tcgttggagc ttcttcgcgg
     4501 caagtcaggg cgtgctttcg gccaaatggc tggtttgatg atggtgagtc ctcatatcaa
     4561 acatctcagc cagtttgact tggcttttga gggtgaccgc ggccgccgtt ccggtaatta
     4621 actggcctca tgggccttca cttcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4681 gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct
     4741 cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc
     4801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5041 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5281 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5581 tatgagtaaa cttggtctga cagttattat ttacccacca ctttggtaat ttcgcctttc
     5641 acgtaatgca caaattcttc cagctgatcc gcacggctgg ccagacgatc ttcttcctgg
     5701 cccagatacg cctgacgcgc ttccagaatc accggctgat actgcgccgg cagacgttcc
     5761 atggcccaat ccgccgccac atctttcggc gcaattttgc cggtcaccgc gctataccaa
     5821 atacggctca gggtcagcac cacgttgcgt tcatcacccg cccaatccgg cgggctgttc
     5881 cacagggtca gggtttcgtt cagcgcttca aacaggtcct gttccggcac cggatcaaac
     5941 agttcttccg ctgccggacc aaccagcgcc acgctatgtt cacgcgcttt ggtcagcaga
     6001 atggccagat cgatatcaat ggtcgccggt tcaaaaatgc cggccagaat atcgttacgc
     6061 tgccattcgc caaactgcag ctcacgtttc gccggataac gccacggaat aatgtcatca
     6121 tgcaccacga tggtcacttc caccgcacgc agaatttcgc tttcacccgg gctcgcgctg
     6181 gtttccagca gatcgttaat cagcgcacga cgggtggttt catccagacg cacggtcacg
     6241 gtcaccagca gatcaatatc gctatgcggt ttcaggccgc catccaccgc gctgccatac
     6301 agatgcacgg ccagcagggt cggttccaga tgacgttcaa tcacgcccac cacttcagac
     6361 agctgggtgc tcacttccgc aatcaccgct tcacgcataa tgttcagttt ggttttcgga
     6421 cggctgccct gctgggtcac atcgttgctg ctccacagca tcaggcaacg gcccaccgca
     6481 taacgcgcct gctgtttgct cgcacgcggc atgctctgca cgccaaaaaa gcaatcatag
     6541 cacgccataa acaccgccac cgcaccatta ccgccgctac gttcggtcag ggtacggctc
     6601 caattacggc tacgcatact cttccttttt caatattatt gaagcattta tcagggttat
     6661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6721 cgcacatttc cccgaaaagt gccac
//
