LOCUS       V008672                 7225 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008672
VERSION     V008672
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Fernandez-Nino MA, Arevalo-Ferro C, Dreyer I.
  TITLE     A synthetic multi-cellular network of coupled oscillators
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Fernandez-Nino MA, Arevalo-Ferro C, Dreyer I.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2014) Biologia, Universidad Nacional de Colombia,
            Avenida Carrera 30 # 45, Bogota, Cundinamarca 111321, Colombia
REFERENCE   3  (bases 1 to 7225)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-JUL-2014) Biologia, Universidad Nacional de Colombia, Avenida
            Carrera 30 # 45, Bogota, Cundinamarca 111321, Colombia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(198..786)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(961..1731)
                     /codon_start=1
                     /product="KanR"
                     /label="KanR"
                     /note="kanamycin resistance gene"
                     /protein_id="AKE33862.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     CDS             1736..1768
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      1786..1872
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(1916..1932)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1936..1991
                     /label="luxbox"
                     /note="luxbox"
                     /regulatory_class="promoter"
     CDS             1998..2576
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             2583..2615
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      2633..2719
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(2763..2779)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2782..3609
                     /gene="aiiB"
                     /label="N-acyl homoserine lactonase AiiB"
                     /note="N-acyl homoserine lactonase AiiB from Agrobacterium
                     fabrum (strain C58 / ATCC 33970). Accession#: A9CKY2"
     CDS             3616..3648
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      3666..3752
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    3733..3815
                     /note="green fluorescent protein; GFP"
     primer_bind     complement(3796..3812)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3817..3849
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      3867..3953
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(3997..4013)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4020..4727
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      4784..4870
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(4914..4930)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4940..4956
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4963..4979
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5021..5032
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             5039..5788
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             5792..5824
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      5842..5928
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5972..5988)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5994..6572
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             6573..6605
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      complement(6626..6669)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(6753..6769)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(6971..7075)
                     /label="AmpR promoter"
     terminator      complement(7165..7208)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ggatcctaac tcgagctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      961 tcaaaatggt atgcgttttg acacatccac tatatatccg tgtcgttctg tccactcctg
     1021 aatcccattc cagaaattct ctagcgattc cagaagtttc tcagagtcgg aaagttgacc
     1081 agacattacg aactggcaca gatggtcata acctgaagga agatctgatt gcttaactgc
     1141 ttcagttaag accgaagcgc tcgtcgtata acagatgcga tgatgcagac caatcaacat
     1201 ggcacctgcc attgctacct gtacagtcaa ggatggtaga aatgttgtcg gtccttgcac
     1261 acgaatatta cgccatttgc ctgcatattc aaacagctct tctacgataa gggcacaaat
     1321 cgcatcgtgg aacgtttggg cttctaccga tttagcagtt tgatacactt tctctaagta
     1381 tccacctgaa tcataaatcg gcaaaataga gaaaaattga ccatgtgtaa gcggccaatc
     1441 tgattccacc tgagatgcat aatctagtag aatctcttcg ctatcaaaat tcacttccac
     1501 cttccactca ccggttgtcc attcatggct gaactctgct tcctctgttg acatgacaca
     1561 catcatctca atatccgaat agggcccatc agtctgacga ccaagagagc cataaacacc
     1621 aatagcctta acatcatccc catatttatc caatattcgt tccttaattt catgaacaat
     1681 cttcattctt tcttctctag tcattattat tggtccattc actattctca tgctagcagc
     1741 aaacgacgaa aactacgctt tagcagctta ataatagaga ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1861 agtaggacaa atccgccgcc ctagacctat ctagagaatt cgatatcaag cttatcgata
     1921 ccgtcgacct cgaggcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
     1981 agtcgaataa atctagaatg actataatga taaaaaaatc ggattttttg gcaattccat
     2041 cggaggagta taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa agacttgagt
     2101 gggacttagt tgtagaaaat aaccttgaat cagatgagta tgataactca aatgcagaat
     2161 atatttatgc ttgtgatgat actgaaaatg taagtggatg ctggcgttta ttacctacaa
     2221 caggtgatta tatgctgaaa agtgtttttc ctgaattgct tggtcaacag agtgctccca
     2281 aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc tccaagataa
     2341 ataactctgc tagtgaaatt acaatgaaac tatttgaagc tatatataaa cacgctgtta
     2401 gtcaaggtat tacagaatat gtaacagtaa catcaacagc aatagagcga tttttaaagc
     2461 gtattaaagt tccttgtcat cgtattggag acaaagaaat tcatgtatta ggtgatacta
     2521 aatcggttgt attgtctatg cctattaatg aacagtttaa aaaagcagtc ttaaattcta
     2581 gagcagcaaa cgacgaaaac tacgctttag cagcttaata atagagaggc atcaaataaa
     2641 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2701 tctcctgagt aggacaaatc cgccgcccta gacctatcta gagaattcga tatcaagctt
     2761 atcgataccg tcgacctcga gatgggaaat aagctgttcg ttctcgacct gggtgaaatt
     2821 cgtgtcgacg aaaacttcat catcgccaac tccacttttg ttacgccgca gaagccgacg
     2881 gtgagtagtc gtctgatcga cattccggtc tccgcctacc tcatccaatg caccgatgcc
     2941 acggtcctct acgacacagg atgtcacccg gaatgcatgg gaacgaatgg ccgatggccc
     3001 gcgcaatcgc aactgaacgc gccctatatc ggggcgtcgg aatgcaatct gccggaacgg
     3061 ctgaggcagc tcggcctttc accggacgat atttcgacgg tggtcctgtc gcatcttcac
     3121 aatgatcacg ccggttgcgt cgagtatttc ggaaagtcga ggctgattgc acatgaagat
     3181 gaattcgcaa ccgcagtccg gtatttcgcg acaggcgatc attcgtcgcc ttacatcgtc
     3241 aaggacatcg aggcatggct tgcgaccccg cgaaactggg atctcgtcgg tcgcgatgag
     3301 agggaaagag aactcgcgcc cggagtgaac ctgctcaact tcggaaccgg ccatgcttca
     3361 ggaatgctgg gcctggctgt caggcttgag aagcagcccg ggtttctgct cgtttcagac
     3421 gcttgttaca ccgccacgaa ctatggtccc cctgcccgcc gagcaggcgt tctccacgac
     3481 acgatcggtt acgatcggac ggtcagccat atcaggcaat acgccgaatc ccgttctctc
     3541 acagtcctct tcggtcacga ccgggagcag tttgccagcc tgatcaaatc cacggacgga
     3601 ttctatgaat ctagagcagc aaacgacgaa aactacgctt tagcagctta ataatagaga
     3661 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     3721 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctat ctagagaatt
     3781 cgatatcaag cttatcgata ccgtcgacct cgagatgcag caaacgacga aaactacgct
     3841 ttagcagctt aataatagag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     3901 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     3961 cctagaccta tctagagaat tcgatatcaa gcttatcgat accgtcgacc tcgaggcgta
     4021 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     4081 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     4141 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     4201 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     4261 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     4321 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     4381 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     4441 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     4501 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     4561 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     4621 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     4681 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaagtct agagcagcaa
     4741 acgacgaaaa ctacgcttta gcagcttaat aatagagagg catcaaataa aacgaaaggc
     4801 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     4861 taggacaaat ccgccgccct agacctatct agagaattcg atatcaagct tatcgatacc
     4921 gtcgacctcg aggctagcat tgtgagcgga taacaattga cattgtgagc ggataacaag
     4981 atactgagca catcagcagg acgcactgac cgaattcatt aaagaggaga aatctagaat
     5041 gaaaaacata aatgccgacg acacttacag aataattaat aaaattaaag cttgtagaag
     5101 caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta
     5161 tttactcgcg atcatttatc ctcattccat ggttaaatct gatatttcaa tcctagataa
     5221 ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat
     5281 agtagattat tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc
     5341 tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa tcatcaggtc ttatcactgg
     5401 gtttagtttc cctattcata ctgctaataa tggcttcgga atgcttagtt ttgcacattc
     5461 agagaaagac aactatatag atagtttatt tttacatgca tgtatgaaca taccattaat
     5521 tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa
     5581 cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa agagctcttg
     5641 ggatatttca aaaatattag gctgcagtaa gcgtacggtc actttccatt taaccaatgc
     5701 gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg
     5761 agcaattgat tgcccatact ttaaaaattc tgcagcaaac gacgaaaact acgctttagc
     5821 agcttaataa tagagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     5881 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     5941 acctatctag agaattcgat atcaagctta tcgataccgt cgacctcgag agaatgacta
     6001 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa
     6061 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc
     6121 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg
     6181 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg
     6241 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa
     6301 gtcgttttgc tgtaggtaaa aatagctcca agataaataa ctctgctagt gaaattacaa
     6361 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa
     6421 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta
     6481 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta
     6541 ttaatgaaca gtttaaaaaa gcagtcttaa atgcagcaaa cgacgaaaac tacgctttag
     6601 cagcttaata atagagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     6661 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     6721 gacctatcta gagaattcga tatcaagctt atcgataccg tcgacctcga gtcattggaa
     6781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7081 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7141 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     7201 atgatttctg gaattcatga gatct
//
