LOCUS       40924_9236       10516 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCC-chi-cat, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Liu X.
  TITLE     A Highly Efficient and Simple Construction Strategy for Producing 
            Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus
  JOURNAL   PLoS ONE 11 (3), E0152140 (2016)
  PUBMED    27008267
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing, Beijing 100081, China
REFERENCE   3  (bases 1 to 10516)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10516)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0152140"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2016) Biotechnology Research Institute, Chinese Academy of 
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 
            Beijing 100081, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             436..447
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    complement(1071..1544)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1620..2588)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2591..3763)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(4089..4339)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4345..5097)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(5188..5407)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(5483..6097)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        6956..7058
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7059..7715
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    7839..8276
                     /label=homologous arm for BmNPV cathepsin gene
                     /note="homologous arm for BmNPV cathepsin gene"
     primer_bind     complement(8295..8311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8319..8335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8343..8373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8388..8409)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8697..9285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9459..10316)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10317..10421)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaaatagaa cgggtccgac
      421 agagtccagc ctcctattga aggaagaatt tttagatgtg ggtttgctaa tttcgccgcc
      481 atcaattgtc caaaattgcc tttgtacggt tcgttccaag cggacacgcc cttttggggt
      541 ttttgtacgg cggcccacgg atcgtggatg gcaactttga aatcttcgcg tcccctgcac
      601 gatcgttgca gagcttcaaa acttccgggt attgttttga gggcgtcgtt tattccatcg
      661 ccaccgcaga tgggtatgaa accgtacaac aagtgcgata aatttggcaa gggaactttg
      721 tctacgggaa agctgcgccc gtacacaccc cactcgacaa agtacgcagc gacaatttta
      781 tcctctcttc tgccgggttt gttgttttcc agccatgtgt attcgagtcg acagcgacac
      841 acttgcatcg gatgcagccc ggttaacgtg ccggcacggc ctgggtaacc aggtattttg
      901 tccacataac cgtgcgcaaa atgttgtgga taagcaggac acagcagcaa tccacagcag
      961 gcatacaacc gcacaccgag gttactccgt tctacaggtt acgacgacat gtcaatactt
     1021 gcccttgaca ggcattgatg gaatcgtagt ctcacgctga tagtctgatc gacaatacaa
     1081 gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggat cgtggtccca
     1141 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg
     1201 acgatacgag tgggaccgtg gttccagact aataatcaga ccgacgatac gagtgggacc
     1261 gtggtcccag actaataatc agaccgacga tacgagtggg accatggtcc cagactaata
     1321 atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgatacg
     1381 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
     1441 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc
     1501 gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat gctttctggc
     1561 ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg gtcgcatcag
     1621 ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca ctcagttgga
     1681 acacgagacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac tgtcgctcca
     1741 ggagcaaact gatgtcgtga gcttaaacta gttcttgatg cagatgacgt tttaagcaca
     1801 gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt tttctgctca
     1861 tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc tttttgaagt
     1921 gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac aactgattta
     1981 ggcaatttgg cggtgttgat acagcgggta ataatcttac gtgaaatatt ttccgcatca
     2041 gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata acgctgacca
     2101 cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc agccatctgc
     2161 tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc agcagcttta
     2221 cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc gaacgccggt
     2281 gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc ctcagtaagc
     2341 agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac actatcaccc
     2401 cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt aatggcatta
     2461 ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc agctggtgtc
     2521 gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat aacaggcgca
     2581 cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt tcattgcaga
     2641 caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt tgatcaatgg
     2701 cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc gtttcacgta
     2761 caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc atccacgggg
     2821 actgagagcc attgctattg ctgtatttgg taagcaaaat acgtacatca ggctcgaacc
     2881 ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaactgc agtgcggagg
     2941 tgtagtcaaa caactcagca ggcgtgggaa caatcagcac atcagcagca catacgacat
     3001 taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca tagtcatgag
     3061 caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt ttaccttcat
     3121 caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa ggaataatgt
     3181 caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt tccccaagat
     3241 agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat ccgtgataca
     3301 ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc cccttcagag
     3361 ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct ttatgggcag
     3421 caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta ccaaacacat
     3481 cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc cgtcctcgaa
     3541 tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata gcctgagaag
     3601 aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt ttcctcgctt
     3661 ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca tgaccagcgt
     3721 ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa tcattgcttt
     3781 gcgttttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg caaagtcatc
     3841 aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata agaagagcac
     3901 atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga gcatagcgag
     3961 cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg ctcagcgcaa
     4021 gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga tagccaattc
     4081 agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc cccgatatca
     4141 ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc tcaaatttgg
     4201 cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg aaggaaacag
     4261 caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc accctcaaat
     4321 ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc ggaaggaaaa
     4381 tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc gcattggagt
     4441 tctgctgttg atctcattaa cacagacctg caggaagcgg cggcggaagt caggcatacg
     4501 ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca gagagacgat
     4561 gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca tggcatacgg
     4621 attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct gtaacccgat
     4681 aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg atggactgtg
     4741 cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg catcctcttc
     4801 agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc gtatatcctt
     4861 actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat ggatctcgca
     4921 gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa cgaacagata
     4981 cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt cgtttgactg
     5041 gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt ccgccatgac
     5101 agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa agagtcaata
     5161 agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca cagggttaag
     5221 ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg aaagggcaat
     5281 ttgtcacaac accttctcta gaaccagcat ggataaaggc ctacaaggcg ctctaaaaaa
     5341 gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat aagtggataa
     5401 ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg ctgttccctg
     5461 gtgcttcctc gctcactcga ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
     5521 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
     5581 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
     5641 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
     5701 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
     5761 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
     5821 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
     5881 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
     5941 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
     6001 gcggccggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc
     6061 catagacggc cgccagccca gcggcgaggg caaccagctc gagggcttcg ccctgtcgct
     6121 cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat
     6181 taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag
     6241 atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg
     6301 acagaacact acttcctata aacgctacac aggctcctga gattaataat gcggatctct
     6361 acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt
     6421 ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca
     6481 aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc
     6541 aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt
     6601 tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata
     6661 cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt
     6721 cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
     6781 cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat
     6841 gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     6901 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     6961 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     7021 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     7081 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     7141 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     7201 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     7261 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     7321 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     7381 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     7441 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     7501 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     7561 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     7621 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     7681 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     7741 gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     7801 attcgatgat aagctgtcaa acatgagaat tggtcgacga tcctttgaaa gcgcctaatt
     7861 attttgaaga atttgttcat cgattcaaca aaaattatag tagcgaagtt gaaaaattgc
     7921 gaagattcaa aattttccaa cacaatttaa atgaaattat caataaaaac caaaacgatt
     7981 cggccaaata tgaaataaac aaattctcgg atttgtccaa agacgaaact atcgcaaaat
     8041 acacaggtct gtctttgcct actcagactc aaaatttttg caaggtcata ctcttagacc
     8101 agccgccggg taaagggccc cttgaatttg actggcgtcg tctcaacaaa gtcactagcg
     8161 taaaaaatca aggaatgtgt ggcgcctgct gggcgtttgc cactctgggc agtttggaaa
     8221 gtcaatttgc aatcaaacat aacgaattga ttaatctgtc ggagcagcaa atgatcaagc
     8281 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8341 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8401 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8461 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8521 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8581 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8641 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8701 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8761 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8821 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8881 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8941 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9001 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9061 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9121 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9181 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9241 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9301 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9361 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9421 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9481 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9541 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9601 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9661 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9721 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9781 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9841 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9901 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9961 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10021 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10081 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10141 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10201 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10261 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10321 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10381 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10441 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10501 acgaggccct ttcgtc
//
