LOCUS       62056_4970        2989 bp DNA     circular SYN 30-JUL-2019
DEFINITION  Cloning vector pCC-3op, complete sequence.
ACCESSION   MH026050
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of 
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of 
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 2989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2018) Department of Biology, The University of Texas at 
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..74
                     /label=pLtetO-3op
                     /note="pLtetO-3op"
                     /regulatory_class="promoter"
     CDS             128..841
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      886..972
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1093..1638
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1800..1894)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1927..2784)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2785..2889)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagtccc tatcagttga tagagattga caccctatca gttgatagag atactcccta
       61 tcagttgata gagagagcac atcagcagga cgcactgacc gaattcatta aagaggagaa
      121 aggtaccatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      361 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      601 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      721 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      781 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg atctctacaa
      841 ataaaagctt gatgggggat cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag
      901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      961 agtaggacaa atccgccgcc ctagacctag taacaactta tatcgtatgg ggctgacttc
     1021 aggtgctaca tttgaagaga taaattgcac tgaaatctag aaatatttta tctgattaat
     1081 aagatgatct tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa
     1141 aaccgccttg cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg
     1201 taactggctt ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc
     1261 gcatgacttc aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt
     1321 ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1381 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
     1441 gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1501 aaggcaggaa caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat
     1561 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcangg
     1621 gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct
     1681 tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc
     1741 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatccct agtgcttgga
     1801 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1861 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa cttggtctga
     1921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2941 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
