LOCUS       62056_4965        2970 bp DNA     circular SYN 30-JUL-2019
DEFINITION  Cloning vector pCC-3, complete sequence.
ACCESSION   MH026049
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of 
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of 
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 2970)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2018) Department of Biology, The University of Texas at 
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..55
                     /label=pLtetO-3spacer
                     /note="pLtetO-3spacer"
                     /regulatory_class="promoter"
     CDS             109..822
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      867..953
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1074..1619
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1781..1875)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1908..2765)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2766..2870)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttga cataaatacc actggcggtg atactcccta tcacgttgat agagagagca
       61 catcagcagg acgcactgac cgaattcatt aaagaggaga aaggtaccat gagtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      781 acagctgctg ggattacaca tggcatggat gatctctaca aataaaagct tgatggggga
      841 tcccatggta cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      961 cctagaccta gtaacaactt atatcgtatg gggctgactt caggtgctac atttgaagag
     1021 ataaattgca ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat
     1081 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     1141 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     1201 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     1261 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     1321 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     1381 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     1441 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     1501 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1561 accactgatt tgagcgtcag atttcgtgat gcttgtcang ggggcggagc ctatggaaaa
     1621 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     1681 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     1741 gtcagtgagc gaggaagcgg aatatatccc tagtgcttgg attctcacca ataaaaaacg
     1801 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1861 ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca atgcttaatc
     1921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2881 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2941 aggcgtatca cgaggccctt tcgtcttcac
//
