LOCUS       62056_4960        2969 bp DNA     circular SYN 30-JUL-2019
DEFINITION  Cloning vector pCC-2, complete sequence.
ACCESSION   MH026048
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of 
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of 
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 2969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2018) Department of Biology, The University of Texas at 
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..54
                     /label=pLtetO-2spacer
                     /note="pLtetO-2spacer"
                     /regulatory_class="promoter"
     CDS             108..821
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      866..952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1073..1618
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1780..1874)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1907..2764)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2765..2869)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttga cataaatacc actggcggtg atactcccta tcagttgata gagagagcac
       61 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg agtaaaggag
      121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      241 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      301 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      361 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      421 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      661 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      721 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      781 cagctgctgg gattacacat ggcatggatg atctctacaa ataaaagctt gatgggggat
      841 cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
      961 ctagacctag taacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga
     1021 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc
     1081 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     1141 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     1201 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     1261 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     1321 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     1381 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     1441 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     1501 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1561 ccactgattt gagcgtcaga tttcgtgatg cttgtcangg gggcggagcc tatggaaaaa
     1621 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     1681 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     1741 tcagtgagcg aggaagcgga atatatccct agtgcttgga ttctcaccaa taaaaaacgc
     1801 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1861 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     1921 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1981 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2041 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2101 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2161 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2221 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2281 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2341 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2401 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2461 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2521 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2581 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2641 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2701 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2761 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2821 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2881 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2941 ggcgtatcac gaggcccttt cgtcttcac
//
