LOCUS       62056_4955        2968 bp DNA     circular SYN 30-JUL-2019
DEFINITION  Cloning vector pCC-1, complete sequence.
ACCESSION   MH026047
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of 
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of 
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 2968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2018) Department of Biology, The University of Texas at 
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    35..53
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             107..820
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDDLYK"
     terminator      865..951
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1072..1617
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1779..1873)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1906..2763)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2764..2868)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttga cataaatacc actggcggtg atactcccta tcagtgatag agagagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gtaaaggaga
      121 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      181 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      241 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      301 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      361 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      421 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      481 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      541 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      601 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      661 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      721 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      781 agctgctggg attacacatg gcatggatga tctctacaaa taaaagcttg atgggggatc
      841 ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagacctagt aacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat
     1021 aaattgcact gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg
     1081 ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt
     1141 ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca
     1201 gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc
     1261 ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg
     1321 actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1381 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac
     1441 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     1501 acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1561 cactgatttg agcgtcagat ttcgtgatgc ttgtcanggg ggcggagcct atggaaaaac
     1621 ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat
     1681 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt
     1741 cagtgagcga ggaagcggaa tatatcccta gtgcttggat tctcaccaat aaaaaacgcc
     1801 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1861 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag
     1921 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1981 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2041 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2101 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2161 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2221 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2281 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2341 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2401 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2461 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2521 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2581 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2641 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2701 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2761 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2821 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2881 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2941 gcgtatcacg aggccctttc gtcttcac
//
