LOCUS       62056_4950        2967 bp DNA     circular SYN 30-JUL-2019
DEFINITION  Cloning vector pCC-0, complete sequence.
ACCESSION   MH026046
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Engineering DNA recognition and allosteric response properties of 
            TetR family proteins by using a module-swapping strategy
  JOURNAL   Nucleic Acids Res. (2019) In press
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Jordan BR, Dimas RP, Jiang X-L., Morcos F, Chan CTY.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2018) Department of Biology, The University of 
            Texas at Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA
REFERENCE   3  (bases 1 to 2967)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2018) Department of Biology, The University of Texas at 
            Tyler, 3900 University Blvd, BEP 133, Tyler, TX 75799, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..52
                     /label=pLtetO-0spacer
                     /note="pLtetO-0spacer"
                     /regulatory_class="promoter"
     CDS             106..819
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      864..950
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1071..1616
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1778..1872)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1905..2762)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2763..2867)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttga cataaatacc actggcggtg atactcccta tcatgataga gagagcacat
       61 cagcaggacg cactgaccga attcattaaa gaggagaaag gtaccatgag taaaggagaa
      121 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      181 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      241 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      301 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      361 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      421 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      481 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      541 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      601 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      661 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      721 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      781 gctgctggga ttacacatgg catggatgat ctctacaaat aaaagcttga tgggggatcc
      841 catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
      961 agacctagta acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata
     1021 aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt
     1081 tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt
     1141 tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag
     1201 tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc
     1261 tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga
     1321 ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
     1381 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
     1441 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     1501 cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 actgatttga gcgtcagatt tcgtgatgct tgtcangggg gcggagccta tggaaaaacg
     1621 gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc
     1681 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     1741 agtgagcgag gaagcggaat atatccctag tgcttggatt ctcaccaata aaaaacgccc
     1801 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1861 tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gttaccaatg cttaatcagt
     1921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2881 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2941 cgtatcacga ggccctttcg tcttcac
//
