LOCUS       V008686                 5920 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008686
VERSION     V008686
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1445
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Marinitoga piezophila"
                     /protein_id="AUE21779.1"
                     /translation="MLLNGAWESKVDIYNVFELRCKTTAYFGVGAIEKFKDICEFLNKK
                     DMKKVLILTDEVVYNVTGIKEKVEKFLGEAGIEYIVYYGIKPNPNVKMIDEAKKMGLDF
                     GAKAVIGIGGGSHIDTAKSVAVLLHEKYKNYTGSDLYELKFVPEEALPIIAINTTHGTG
                     TEVDRFAVATIEEKGYKPALAYDSIYPIFAIDDPEITKSLPWSQTTYTAIDAINHVTEA
                     ATTLAASPYSILLAKETIRLVSKYLPQAQAKPGDLTARYFLLYASTIAGISFDNGLLHF
                     THALEHPLSGIRPDLPHGLGLAMLLPAVVKAIYPAQAEVLAEIYSPIVPGLKGVPGEAE
                     EAAKGIEKWLFNIGVTQKLTDEGFKESDIDKLVELAFNTPSLDLLLSLAPVKATKEIVK
                     QIYMDSLYPLNK"
     gene            210..1445
                     /gene="adhA"
                     /label="adhA"
     CDS             1575..2222
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2306..3163)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3164..3255)
                     /label="AmpR promoter"
     rep_origin      3855..4400
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4871..5872
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgttattaaa tggtgcatgg gagagtaaag
      241 tagatattta caacgttttt gagttaagat gtaaaacaac agcttatttt ggagtaggtg
      301 ctatagaaaa gttcaaggat atttgtgaat ttttaaacaa aaaggatatg aaaaaggttt
      361 taattttaac cgacgaagtt gtatataacg tgacaggaat aaaagaaaaa gttgaaaagt
      421 ttttaggtga agctggtatt gaatatattg tatattatgg aattaaacca aatccaaatg
      481 taaaaatgat tgatgaagca aagaaaatgg gattagattt tggagcaaaa gctgttatag
      541 gaattggtgg cggaagtcat attgacacag caaagagtgt tgcagtatta ttacatgaaa
      601 aatataagaa ttatacaggt tctgacttat atgaattaaa atttgtgcca gaagaagcat
      661 tgccaataat tgcaataaat acaactcatg gtacaggtac tgaagttgat agatttgcag
      721 ttgcaacaat tgaggaaaaa ggttataaac cagccttagc ttatgatagt atatatccaa
      781 tatttgctat tgatgatcca gaaattacaa aatcattacc atggtcacaa acaacataca
      841 cagctattga tgcaataaac catgttacag aagcagcaac aacattagca gcatccccat
      901 attcaatttt attagctaaa gaaacaataa gattagtttc aaaatatctt cctcaagctc
      961 aggcaaaacc aggagattta acagcaagat atttcttatt atatgcttca acaatagctg
     1021 gtatttcatt tgataatgga ttgttacatt tcacacatgc tttagaacat ccactaagtg
     1081 gaatcagacc agatttacca catggtttag gattagcaat gttattacca gcagtagtaa
     1141 aagcaattta tcctgcacag gcagaagtgc ttgcagaaat atatagtcca attgttccag
     1201 gtttaaaagg agtgccgggt gaagcagaag aagcagcaaa aggaattgaa aaatggttat
     1261 ttaatattgg agttacacaa aaattaacag atgaaggatt taaagaaagc gatatagaca
     1321 aattagtaga attggcattt aatacacctt cacttgattt attattgagc ttagcacctg
     1381 ttaaagctac aaaagaaatt gtaaaacaga tttatatgga ttcattatat cctttaaata
     1441 aataattaat agaaataatt ttgtttaact ttacaaacgg gattgacttt taaaaaagga
     1501 ttgattctaa tgaagaaagc agacaagtaa gcctcctaaa ttcactttag ataaaaattt
     1561 aggaggcata tcaaatgaac tttaataaaa ttgatttaga caattggaag agaaaagaga
     1621 tatttaatca ttatttgaac caacaaacga cttttagtat aaccacagaa attgatatta
     1681 gtgttttata ccgaaacata aaacaagaag gatataaatt ttaccctgca tttattttct
     1741 tagtgacaag ggtgataaac tcaaatacag cttttagaac tggttacaat agcgacggag
     1801 agttaggtta ttgggataag ttagagccac tttatacaat ttttgatggt gtatctaaaa
     1861 cattctctgg tatttggact cctgtaaaga atgacttcaa agagttttat gatttatacc
     1921 tttctgatgt agagaaatat aatggttcgg ggaaattgtt tcccaaaaca cctatacctg
     1981 aaaatgcttt ttctctttct attattccat ggacttcatt tactgggttt aacttaaata
     2041 tcaataataa tagtaattac cttctaccca ttattacagc aggaaaattc attaataaag
     2101 gtaattcaat atatttaccg ctatctttac aggtacatca ttctgtttgt gatggttatc
     2161 atgcaggatt gtttatgaac tctattcagg aattgtcaga taggcctaat gactggcttt
     2221 tataatatga gataatgccg actgtacttt ttacagtcgg ttttctaatg tcactagggc
     2281 tcgcctttgg gaagtttgaa ggttaccaat gcttaatcag tgaggcacct atctcagcga
     2341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2581 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     2641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     2941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3241 agaaaaataa acaaaagagt ttgaggaaaa cttaagaaaa cgggaaacta tttcctgttg
     3301 agaatgtgaa attaaatgat aaaaagtgat ataatagggt caggcgtaca gaagcctgac
     3361 ctttttgcaa aattgtttta ttggcgcggc gtatatatac agcatatata caatgtaaat
     3421 ttatacaatc aaattgttga tacaatcgaa ttatgcggct aaaaatttgc tcgcgattgc
     3481 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt
     3541 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa
     3601 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt
     3661 gatcttctca aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa
     3721 cccccctttg ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa
     3781 agaaaggtct taaaggtttt atggttttgg tcatgagcct agaaatattt tatctgatta
     3841 ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa
     3901 aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga
     3961 ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg
     4021 gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc
     4081 ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4141 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     4201 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     4261 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
     4321 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     4381 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     4441 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     4501 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat
     4561 attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca
     4621 ccctcatcag tgccaacata gtaagccagt atacaaatga gtgctttttt tgcgttttga
     4681 gcgtagcgaa aaacgagttc tttctattct tgatacatat agaaataacg tcatttttat
     4741 tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg tgttttaaag tattgaaaac
     4801 ccttaaaatt ggttgcacag aaaaacccca tctgttaaag ttataagtga ccaaacaaat
     4861 aactaaatag atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga
     4921 tgaaaaatca agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga
     4981 aaagaaaatc gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc
     5041 tgaaagagta aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga
     5101 aagaaagttg tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag
     5161 gagagcaatg aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa
     5221 gccaacagtt cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga
     5281 attaaataag agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa
     5341 aattaataaa aatcttgttg gttttatgcg tgcaacggaa gtgacaataa ataataaaga
     5401 taattcttat aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt attttaagaa
     5461 tacagaaaac tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg caatgaaatt
     5521 agactatgat ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat ataaatcgga
     5581 tatacaatcg gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac
     5641 cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa
     5701 aaggttaatc tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga
     5761 cacagaagaa ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt
     5821 ttctattatt gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa
     5881 cccggggatc ctctagagtc gacctgcagg catgcaagct
//
