LOCUS       V008687                 5899 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008687
VERSION     V008687
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5899)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5899)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1424
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Methanocaldococcus bathoardescens"
                     /protein_id="AUE21772.1"
                     /translation="MNYEKEVNINNVFELRCKTTDYFGVGAIHKFDDIAKEFVEKGMKN
                     FLFVTGKSSYKKCGAWDVIEPILDKYGINYELYNKVSANPTVDMIDEAVKLGKEIESQV
                     VIGIGGGSPLDTAKSVAVLLEYPDKTGRDLYEFKFAPERAKPIVAINTTHGTGSEVDRF
                     AVATVPEKNYKPAIVYDCIYPTYAIDDPALMTKLPKEQALATAIDAVNHVNEAATTKVA
                     SPYVVDLARRTVGLIKEYLPRVLENPEDLTARYWVLYASTIAGIALDNGLAHITHALEH
                     PLSAIDANVTHGIGLAALMPAVVKYCHPALPKTFAYIYEPIVEDKERAEKDANYLAQEI
                     EKWLFDLGLTQKLPDLGFKESDVDELTRLAMTTPSLDILLSLAPIEVNEDVVRNIYLDS
                     MQPIQ"
     gene            210..1424
                     /gene="adhA"
                     /label="adhA"
     CDS             1554..2201
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2285..3142)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3143..3234)
                     /label="AmpR promoter"
     rep_origin      3834..4379
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4850..5851
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgaactacga aaaagaagta aatataaaca
      241 acgtatttga attaagatgc aaaacaacag attactttgg agttggagca atacacaaat
      301 ttgatgacat tgcaaaagaa ttcgttgaaa aaggcatgaa aaacttcctc tttgtaacag
      361 gaaaaagcag ttacaaaaaa tgtggagcat gggatgtaat agaaccaatc ttagacaagt
      421 atggaataaa ctatgaatta tacaacaaag tatcagccaa cccaacagtt gatatgattg
      481 acgaagcagt aaaattagga aaagaaatag aaagtcaagt tgttattgga attggtggag
      541 gaagcccatt agacactgca aaaagcgtgg cagtattatt agaataccca gacaagacag
      601 gtagggactt atacgaattc aaatttgctc cagaaagggc aaagccaatt gtagcaataa
      661 acactacaca tggaactgga agtgaggtag ataggtttgc agttgctact gttccagaga
      721 agaactataa accagcaatt gtttatgatt gcatttaccc aacctatgca attgatgacc
      781 ctgcattaat gacaaaactt ccaaaagagc aagctctcgc tacagcaatt gatgctgtga
      841 accacgttaa tgaagctgca acaacaaaag tggcaagccc ttatgttgtt gatttggcaa
      901 ggagaactgt aggattgata aaagagtatt taccaagagt tttagaaaat ccagaagatt
      961 taactgcgag atattgggtt ttatatgcct ctacaattgc aggaattgct ttggataatg
     1021 gtttagctca cattactcat gctttagaac acccattgag tgctattgac gctaatgtta
     1081 ctcatggtat tgggttagcg gcattaatgc ctgcagttgt aaaatactgt catccagcat
     1141 tgccaaagac ttttgcttat atttatgagc caatagttga ggataaggag agggctgaaa
     1201 aggatgcaaa ctatcttgct caagagattg agaagtggtt gtttgatttg ggcttaactc
     1261 aaaaactacc tgatttagga tttaaagaaa gtgatgttga tgaattgacg agattggcaa
     1321 tgactacccc aagtttggat atcttgttat ctttagcacc tattgaagtt aatgaggatg
     1381 ttgttagaaa tatttactta gattcaatgc aacccattca ataattaata gaaataattt
     1441 tgtttaactt tacaaacggg attgactttt aaaaaaggat tgattctaat gaagaaagca
     1501 gacaagtaag cctcctaaat tcactttaga taaaaattta ggaggcatat caaatgaact
     1561 ttaataaaat tgatttagac aattggaaga gaaaagagat atttaatcat tatttgaacc
     1621 aacaaacgac ttttagtata accacagaaa ttgatattag tgttttatac cgaaacataa
     1681 aacaagaagg atataaattt taccctgcat ttattttctt agtgacaagg gtgataaact
     1741 caaatacagc ttttagaact ggttacaata gcgacggaga gttaggttat tgggataagt
     1801 tagagccact ttatacaatt tttgatggtg tatctaaaac attctctggt atttggactc
     1861 ctgtaaagaa tgacttcaaa gagttttatg atttatacct ttctgatgta gagaaatata
     1921 atggttcggg gaaattgttt cccaaaacac ctatacctga aaatgctttt tctctttcta
     1981 ttattccatg gacttcattt actgggttta acttaaatat caataataat agtaattacc
     2041 ttctacccat tattacagca ggaaaattca ttaataaagg taattcaata tatttaccgc
     2101 tatctttaca ggtacatcat tctgtttgtg atggttatca tgcaggattg tttatgaact
     2161 ctattcagga attgtcagat aggcctaatg actggctttt ataatatgag ataatgccga
     2221 ctgtactttt tacagtcggt tttctaatgt cactagggct cgcctttggg aagtttgaag
     2281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2581 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2881 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaaagagtt
     3241 tgaggaaaac ttaagaaaac gggaaactat ttcctgttga gaatgtgaaa ttaaatgata
     3301 aaaagtgata taatagggtc aggcgtacag aagcctgacc tttttgcaaa attgttttat
     3361 tggcgcggcg tatatataca gcatatatac aatgtaaatt tatacaatca aattgttgat
     3421 acaatcgaat tatgcggcta aaaatttgct cgcgattgct gaataaaaga tacgagagac
     3481 ctctcttgta tcttttttat tttgagtggt tttgtccgtt acactagaaa accgaaagac
     3541 aataaaaatt ttattcttgc tgagtctggc tttcggtaag ctagacaaaa cggacaaaat
     3601 aaaaattggc aagggtttaa aggtggagat tttttgagtg atcttctcaa aaaatactac
     3661 ctgtcccttg ctgattttta aacgagcacg agagcaaaac ccccctttgc tgaggtggca
     3721 gagggcaggt ttttttgttt cttttttctc gtaaaaaaaa gaaaggtctt aaaggtttta
     3781 tggttttggt catgagccta gaaatatttt atctgattaa taagatgatc ttcttgagat
     3841 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     3901 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     3961 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     4021 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     4081 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     4141 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     4201 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     4261 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     4381 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     4441 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     4501 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc
     4561 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag
     4621 taagccagta tacaaatgag tgcttttttt gcgttttgag cgtagcgaaa aacgagttct
     4681 ttctattctt gatacatata gaaataacgt catttttatt ttagttgctg aaaggtgcgt
     4741 tgaagtgttg gtatgtatgt gttttaaagt attgaaaacc cttaaaattg gttgcacaga
     4801 aaaaccccat ctgttaaagt tataagtgac caaacaaata actaaataga tgggggtttc
     4861 ttttaatatt atgtgtccta atagtagcat ttattcagat gaaaaatcaa gggttttagt
     4921 ggacaagaca aaaagtggaa aagtgagacc atggagagaa aagaaaatcg ctaatgttga
     4981 ttactttgaa cttctgcata ttcttgaatt taaaaaggct gaaagagtaa aagattgtgc
     5041 tgaaatatta gagtataaac aaaatcgtga aacaggcgaa agaaagttgt atcgagtgtg
     5101 gttttgtaaa tccaggcttt gtccaatgtg caactggagg agagcaatga aacatggcat
     5161 tcagtcacaa aaggttgttg ctgaagttat taaacaaaag ccaacagttc gttggttgtt
     5221 tctcacatta acagttaaaa atgtttatga tggcgaagaa ttaaataaga gtttgtcaga
     5281 tatggctcaa ggatttcgcc gaatgatgca atataaaaaa attaataaaa atcttgttgg
     5341 ttttatgcgt gcaacggaag tgacaataaa taataaagat aattcttata atcagcacat
     5401 gcatgtattg gtatgtgtgg aaccaactta ttttaagaat acagaaaact acgtgaatca
     5461 aaaacaatgg attcaatttt ggaaaaaggc aatgaaatta gactatgatc caaatgtaaa
     5521 agttcaaatg attcgaccga aaaataaata taaatcggat atacaatcgg caattgacga
     5581 aactgcaaaa tatcctgtaa aggatacgga ttttatgacc gatgatgaag aaaagaattt
     5641 gaaacgtttg tctgatttgg aggaaggttt acaccgtaaa aggttaatct cctatggtgg
     5701 tttgttaaaa gaaatacata aaaaattaaa ccttgatgac acagaagaag gcgatttgat
     5761 tcatacagat gatgacgaaa aagccgatga agatggattt tctattattg caatgtggaa
     5821 ttgggaacgg aaaaattatt ttattaaaga gtagttcaac ccggggatcc tctagagtcg
     5881 acctgcaggc atgcaagct
//
