LOCUS       V008688                 5761 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008688
VERSION     V008688
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5761)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1286
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Thermotoga petrophila"
                     /protein_id="AUE21775.1"
                     /translation="MVVVSDKVAYKVTGAWDKVKPALEKRGIEYVVYDDVTPNPTTEQI
                     NEATKKALEINAQAVIGIGGGSPIDTAKSVAILLEYPEKTAEELYEQKFIPEKAKPIIA
                     INTTHGTGTEVDRFAVASILEKEYKPPIAYDCIYPTFSIDDPELTKSLPEKQSIYTSLD
                     ALNHITEAATTSLGNPFSINLARNTVELISEYLPEVLKKPDNLEARYFLMYASAIAGIS
                     FDNGMLHLTHALEHPLSAIKPDLPHGLGLAMLLPAVFKYTYKAKPQILAYVYQPIIPDL
                     KGRPNEAEEAVSKLEQWIFNLGVKEKLADVGFSEKDIPKLVKLAFETPALDLLLNLAPM
                     KVDENLVEAIYKESLKPY"
     gene            210..1286
                     /gene="adhA"
                     /label="adhA"
     CDS             1416..2063
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2147..3004)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3005..3096)
                     /label="AmpR promoter"
     rep_origin      3696..4241
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4712..5713
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgttg tggtagttgt tagtgacaaa gttgcgtaca
      241 aagttacagg tgcatgggat aaagtgaagc ctgctttgga aaagagaggt atagaatacg
      301 tagtttatga tgatgtgaca ccaaatccca caaccgaaca gataaacgag gctacaaaaa
      361 aagcgcttga aataaacgct caagccgtga taggaatagg cggaggaagc cccatcgaca
      421 ctgcaaaaag tgtcgcaata ttacttgagt atcctgaaaa aacagctgaa gaattgtatg
      481 aacaaaagtt catacccgaa aaagcaaaac ccataattgc cataaacaca acacatggta
      541 caggaacaga agtcgataga tttgcagtgg catctattct ggaaaaagaa tacaaaccac
      601 caatagccta tgattgcatt tatccaacct tctctatcga tgatccagag ctaactaaaa
      661 gtcttccaga aaaacaaagt atttatacct ctcttgatgc actgaatcat atcacagaag
      721 cagctacaac ttctttagga aatccatttt caataaatct tgcaagaaat actgtggaat
      781 tgatatctga atacttaccc gaggttctca aaaaacctga taacttagaa gcgcgttact
      841 tcctgatgta tgcttctgca atagcaggta tctctttcga taatggtatg ctacatctca
      901 cgcatgctct tgaacatccg ctgagtgcta tcaaacccga tcttcctcat ggtttgggac
      961 ttgctatgct gcttccagct gtttttaaat acacatataa agcgaaacca caaatacttg
     1021 cttacgtata tcagcctata attcctgatt taaaaggaag accgaatgaa gcggaagaag
     1081 ctgtttcaaa acttgaacag tggattttta acttgggtgt caaagagaaa ctggcggatg
     1141 ttggattttc tgaaaaagac atccccaaac ttgtaaaact tgcttttgaa actcctgcat
     1201 tagatcttct gctgaatttg gcaccaatga aagtagatga gaatctggtg gaagcaatct
     1261 ataaagaatc cttgaagcct tattgattaa tagaaataat tttgtttaac tttacaaacg
     1321 ggattgactt ttaaaaaagg attgattcta atgaagaaag cagacaagta agcctcctaa
     1381 attcacttta gataaaaatt taggaggcat atcaaatgaa ctttaataaa attgatttag
     1441 acaattggaa gagaaaagag atatttaatc attatttgaa ccaacaaacg acttttagta
     1501 taaccacaga aattgatatt agtgttttat accgaaacat aaaacaagaa ggatataaat
     1561 tttaccctgc atttattttc ttagtgacaa gggtgataaa ctcaaataca gcttttagaa
     1621 ctggttacaa tagcgacgga gagttaggtt attgggataa gttagagcca ctttatacaa
     1681 tttttgatgg tgtatctaaa acattctctg gtatttggac tcctgtaaag aatgacttca
     1741 aagagtttta tgatttatac ctttctgatg tagagaaata taatggttcg gggaaattgt
     1801 ttcccaaaac acctatacct gaaaatgctt tttctctttc tattattcca tggacttcat
     1861 ttactgggtt taacttaaat atcaataata atagtaatta ccttctaccc attattacag
     1921 caggaaaatt cattaataaa ggtaattcaa tatatttacc gctatcttta caggtacatc
     1981 attctgtttg tgatggttat catgcaggat tgtttatgaa ctctattcag gaattgtcag
     2041 ataggcctaa tgactggctt ttataatatg agataatgcc gactgtactt tttacagtcg
     2101 gttttctaat gtcactaggg ctcgcctttg ggaagtttga aggttaccaa tgcttaatca
     2161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     2461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2761 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3061 gatacatatt tgaatgtatt tagaaaaata aacaaaagag tttgaggaaa acttaagaaa
     3121 acgggaaact atttcctgtt gagaatgtga aattaaatga taaaaagtga tataataggg
     3181 tcaggcgtac agaagcctga cctttttgca aaattgtttt attggcgcgg cgtatatata
     3241 cagcatatat acaatgtaaa tttatacaat caaattgttg atacaatcga attatgcggc
     3301 taaaaatttg ctcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt
     3361 attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt
     3421 gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt
     3481 aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt
     3541 taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt
     3601 ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcatgagcc
     3661 tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta
     3721 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
     3781 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
     3841 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
     3901 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
     3961 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     4021 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     4081 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
     4141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     4201 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
     4261 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
     4321 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     4381 ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc
     4441 acatgaagca cttcactgac accctcatca gtgccaacat agtaagccag tatacaaatg
     4501 agtgcttttt ttgcgttttg agcgtagcga aaaacgagtt ctttctattc ttgatacata
     4561 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     4621 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     4681 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     4741 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4801 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4861 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4921 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4981 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     5041 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     5101 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaggatttcg
     5161 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     5221 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     5281 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     5341 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     5401 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     5461 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     5521 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     5581 taaaaaatta aaccttgatg acacagaaga aggcgatttg attcatacag atgatgacga
     5641 aaaagccgat gaagatggat tttctattat tgcaatgtgg aattgggaac ggaaaaatta
     5701 ttttattaaa gagtagttca acccggggat cctctagagt cgacctgcag gcatgcaagc
     5761 t
//
