LOCUS       V008689                 5905 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008689
VERSION     V008689
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1430
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Thermococcus sp. strain AN1"
                     /protein_id="AUE21777.1"
                     /translation="MLWESQIPINQIFELRCRTIDYFGVGAINKFYDIAKDLKENRGIS
                     RVILVTGKSSYKKCGAWDVVKPALEENGIEYVHYDKVGPNPTVDMVDEAAQMGKEFGAQ
                     AVIGIGGGSPIDTAKSVAILLEYTDKTARELYKLQFSPRKAKPIIAINTTHGTGTEVNR
                     FAVASILEEEYKPAIAYDCIYPLYSIDDPTLMTKLPADQTRYVTIDALNHVNEAATTKV
                     ASPYSILLAKETARLIFDYLPEALIHPDNLQAKYYLLYASAIAGISFDNGLLHFTHALE
                     HPLSAVKPDLPHGLGLAILLPAVIKHIYPATARILAEIYRPLVPEVKGVPGEAELVAKK
                     VEEWLFAIGITQKLTDVGFSEDDVKRLTELAMTTPSLNLLLSLAPVEATKETIEAIYRD
                     SLYPLSK"
     gene            210..1430
                     /gene="adhA"
                     /label="adhA"
     CDS             1560..2207
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2291..3148)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3149..3240)
                     /label="AmpR promoter"
     rep_origin      3840..4385
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4856..5857
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgttgtggga atcccaaatc cccataaacc
      241 aaatttttga gctccgctgc aggactattg actattttgg tgttggtgca attaacaagt
      301 tctatgacat agccaaagac cttaaggaaa accgcggcat aagtagagtt atcctcgtca
      361 ctggaaaaag ctcatacaag aagtgtggcg cttgggatgt tgttaaacca gccctcgagg
      421 aaaacggcat tgagtatgtt cactacgaca aagttggtcc aaacccaaca gttgacatgg
      481 tcgatgaagc cgcacaaatg ggaaaggaat tcggagcaca ggctgttata ggaatcggtg
      541 ggggaagtcc aatagacact gccaagagcg ttgctatttt gcttgagtat accgataaaa
      601 ccgcaagaga gctctacaag ctccagttct ccccaagaaa agcaaagcca attattgcca
      661 taaacaccac tcatggtact ggaactgagg ttaacagatt tgcagttgct tcaatccttg
      721 aggaggagta caaaccagcc atagcttacg actgtatcta cccgctctac tccatcgatg
      781 accccacact tatgacaaag cttccagctg atcagacccg ctatgtgaca attgatgccc
      841 tcaaccatgt caacgaggcc gctacaacaa aggttgcctc accatattca atacttcttg
      901 caaaggaaac tgccaggctt atctttgact atcttccaga ggccttaatt cacccagaca
      961 accttcaagc aaagtactac ctcctctacg cttcagcaat agctggaatt agcttcgata
     1021 atggtctgct ccacttcacc cacgctctcg agcacccgct cagcgctgta aagccagacc
     1081 ttccacatgg tcttggtctt gcaattctct taccagcagt tatcaagcac atttacccag
     1141 ccactgcaag gatacttgct gagatataca ggccactcgt tccagaagtt aaaggagttc
     1201 cgggagaggc agagctcgta gccaagaagg ttgaagagtg gctgtttgct atcggcatta
     1261 cccagaaact cactgacgtt ggattctctg aggatgacgt gaaaaggctt acagaactcg
     1321 caatgacaac accaagcctc aacctgctcc tgtcccttgc accagttgag gctacaaaag
     1381 agaccattga agcaatttat cgcgactcac tttatccgct tagcaagtga ttaatagaaa
     1441 taattttgtt taactttaca aacgggattg acttttaaaa aaggattgat tctaatgaag
     1501 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     1561 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     1621 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     1681 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     1741 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     1801 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     1861 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     1921 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     1981 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     2041 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     2101 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     2161 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     2221 tgccgactgt actttttaca gtcggttttc taatgtcact agggctcgcc tttgggaagt
     2281 ttgaaggtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2581 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2881 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     2941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3241 agagtttgag gaaaacttaa gaaaacggga aactatttcc tgttgagaat gtgaaattaa
     3301 atgataaaaa gtgatataat agggtcaggc gtacagaagc ctgacctttt tgcaaaattg
     3361 ttttattggc gcggcgtata tatacagcat atatacaatg taaatttata caatcaaatt
     3421 gttgatacaa tcgaattatg cggctaaaaa tttgctcgcg attgctgaat aaaagatacg
     3481 agagacctct cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg
     3541 aaagacaata aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga
     3601 caaaataaaa attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa
     3661 tactacctgt cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag
     3721 gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag
     3781 gttttatggt tttggtcatg agcctagaaa tattttatct gattaataag atgatcttct
     3841 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
     3901 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
     3961 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
     4021 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
     4081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     4141 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     4201 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     4261 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     4381 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     4441 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     4501 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     4561 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     4621 acatagtaag ccagtataca aatgagtgct ttttttgcgt tttgagcgta gcgaaaaacg
     4681 agttctttct attcttgata catatagaaa taacgtcatt tttattttag ttgctgaaag
     4741 gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta aaattggttg
     4801 cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta aatagatggg
     4861 ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa aatcaagggt
     4921 tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga aaatcgctaa
     4981 tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga
     5041 ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg
     5101 agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag caatgaaaca
     5161 tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa cagttcgttg
     5221 gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa ataagagttt
     5281 gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct
     5341 tgttggtttt atgcgtgcaa cggaagtgac aataaataat aaagataatt cttataatca
     5401 gcacatgcat gtattggtat gtgtggaacc aacttatttt aagaatacag aaaactacgt
     5461 gaatcaaaaa caatggattc aattttggaa aaaggcaatg aaattagact atgatccaaa
     5521 tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa tcggatatac aatcggcaat
     5581 tgacgaaact gcaaaatatc ctgtaaagga tacggatttt atgaccgatg atgaagaaaa
     5641 gaatttgaaa cgtttgtctg atttggagga aggtttacac cgtaaaaggt taatctccta
     5701 tggtggtttg ttaaaagaaa tacataaaaa attaaacctt gatgacacag aagaaggcga
     5761 tttgattcat acagatgatg acgaaaaagc cgatgaagat ggattttcta ttattgcaat
     5821 gtggaattgg gaacggaaaa attattttat taaagagtag ttcaacccgg ggatcctcta
     5881 gagtcgacct gcaggcatgc aagct
//
