LOCUS       V008692                 5884 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008692
VERSION     V008692
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5884)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1409
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AUE21780.1"
                     /translation="MWETKVNPSKIFELRCKNTTYFGVGSIHKIKDILENLKINGINNV
                     IFITGKGSYKTSGAWDVVRPVLEELDLKYSLYDKVGPNPTVDMIDEAAKIGRESGAKAV
                     IGIGGGSPIDTAKSVAVLLKYTDKNARELYKQKFIPDDAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMFAIDDPSLMTKLDKKQTIAVTVDALNHITEAATTLVAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNIVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEVLADVYSPIVPGLKGLPVEAEYVAEKVQ
                     EWLFSVGCIQKLSDFGFTKDDIPNLVKLAKTTPSLDGLLSIAPVEATESVIEKIYLKSL
                     "
     gene            210..1409
                     /gene="adhA"
                     /label="adhA"
     CDS             1539..2186
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2270..3127)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3128..3219)
                     /label="AmpR promoter"
     rep_origin      3819..4364
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4835..5836
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgtgggaaac aaaagtaaat cctagcaaaa
      241 tttttgaact tcgctgtaaa aatacgactt attttggtgt tggcagtata cataagataa
      301 aggatatatt agaaaatcta aaaattaatg gcattaataa tgttattttt ataacaggca
      361 aaggttcgta taagactagc ggagcttggg atgttgtaag gcctgtattg gaagagcttg
      421 atttaaaata ttcactttac gataaagttg gtccaaatcc taccgttgat atgatagacg
      481 aagcggcaaa aattggacga gaatctggag cgaaagccgt aattggaatt ggcggtggta
      541 gccctataga tactgcaaaa agtgtagctg tgctattaaa gtatactgat aaaaatgcac
      601 gagagcttta taagcaaaaa tttattcctg atgatgctgt tcctataata gctataaatc
      661 ttacacatgg tacaggaaca gaggtagata gatttgcagt ggctactata cctgagaaaa
      721 attataaacc agctatagct tatgattgct tatatcctat gtttgctata gatgaccctt
      781 cgcttatgac aaagcttgat aaaaaacaga ctatagccgt tactgttgat gctttaaatc
      841 acattacaga agctgctacg acacttgttg catctccata tagtattctt acggctaagg
      901 aaacagtaag attgattgtt cgatatcttc ccgctgctgt aaatgatcct ttaaatatag
      961 tggcaagata ttaccttctg tatgcttctg cattagctgg catatctttt gacaatggtc
     1021 ttttgcatct tactcatgct ttagagcatc ctttaagtgc tgtaaaacct gaaatagctc
     1081 atggtttagg gcttggtgca atattgccag cagttataaa agctatttat ccagctacag
     1141 cagaagtatt ggctgatgta tatagtccta tagttcctgg tttaaaagga ctgcctgttg
     1201 aggcggagta tgtagcagaa aaagttcaag aatggctttt tagtgtgggg tgcattcaaa
     1261 agttatctga ttttgggttt acgaaagatg atattcctaa tcttgtaaaa cttgctaaaa
     1321 caactccttc ccttgatggg ttgttatcta ttgcacctgt tgaagctact gaatctgtca
     1381 ttgaaaagat ttatttaaaa tctctttaat taatagaaat aattttgttt aactttacaa
     1441 acgggattga cttttaaaaa aggattgatt ctaatgaaga aagcagacaa gtaagcctcc
     1501 taaattcact ttagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt
     1561 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
     1621 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
     1681 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
     1741 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
     1801 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
     1861 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
     1921 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
     1981 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
     2041 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
     2101 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
     2161 cagataggcc taatgactgg cttttataat atgagataat gccgactgta ctttttacag
     2221 tcggttttct aatgtcacta gggctcgcct ttgggaagtt tgaaggttac caatgcttaa
     2281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2581 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2881 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3181 gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgagg aaaacttaag
     3241 aaaacgggaa actatttcct gttgagaatg tgaaattaaa tgataaaaag tgatataata
     3301 gggtcaggcg tacagaagcc tgaccttttt gcaaaattgt tttattggcg cggcgtatat
     3361 atacagcata tatacaatgt aaatttatac aatcaaattg ttgatacaat cgaattatgc
     3421 ggctaaaaat ttgctcgcga ttgctgaata aaagatacga gagacctctc ttgtatcttt
     3481 tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa aaattttatt
     3541 cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa ttggcaaggg
     3601 tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc ccttgctgat
     3661 ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg caggtttttt
     3721 tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt ttggtcatga
     3781 gcctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     3841 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     3901 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     3961 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     4021 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     4081 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     4141 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     4201 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     4261 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     4321 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     4381 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     4441 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     4501 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     4561 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacaa
     4621 atgagtgctt tttttgcgtt ttgagcgtag cgaaaaacga gttctttcta ttcttgatac
     4681 atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg
     4741 tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt
     4801 aaagttataa gtgaccaaac aaataactaa atagatgggg gtttctttta atattatgtg
     4861 tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag
     4921 tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact ttgaacttct
     4981 gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta
     5041 taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag
     5101 gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt
     5161 tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt
     5221 taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt
     5281 tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac
     5341 ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg tattggtatg
     5401 tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca
     5461 attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg
     5521 accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc
     5581 tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga
     5641 tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat
     5701 acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga
     5761 cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa
     5821 ttattttatt aaagagtagt tcaacccggg gatcctctag agtcgacctg caggcatgca
     5881 agct
//
