LOCUS       V008693                 5896 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008693
VERSION     V008693
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5896)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1421
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Caldicellulosiruptor saccharolyticus"
                     /protein_id="AUE21776.1"
                     /translation="MWETKINPFKVFELRCKNTTYFGIGAINKIGDILEDLKKRGINKV
                     ILITGKSSYKISGAWDVVRPALEEKKVDFCIYDKVGPNPTVDMIEEAVEMAKSFGAQAV
                     IGIGGGSPIDTAKSVAVLLEYKDRNARELYEQKFAPEKAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCIYPLYSIDDPQLMTKLDKTQTVAVTVDALNHVTEASTTLVAS
                     PYSILTAQETVRLIAKYLPVAINEPQNLIARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPELPHGLGLGALLPAIVKTIYPYQAEVLASVYEPIVPNLKGVPSEAEFAAKKVE
                     EWLFEMGCDKKLSDFGFSQSDVDNLVDLALETPSLGLLLSMSPVKATKEVIKKIYEESL
                     YPMK"
     gene            210..1421
                     /gene="adhA"
                     /label="adhA"
     CDS             1551..2198
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2282..3139)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3140..3231)
                     /label="AmpR promoter"
     rep_origin      3831..4376
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4847..5848
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgtgggaaac taaaataaat cctttcaagg
      241 tatttgaact taggtgtaaa aacacaacat atttcggaat tggtgctatt aataaaatag
      301 gcgacatttt ggaggatttg aagaagaggg gaataaataa agttatactt ataactggca
      361 aaagctcgta caaaataagt ggagcttggg atgttgtaag gcctgcactg gaagaaaaga
      421 aagttgactt ttgtatttat gacaaagttg ggccaaatcc aactgttgat atgatcgaag
      481 aagcggttga aatggctaaa agttttggtg cacaagcagt tattggtatc ggtggaggaa
      541 gtccaattga tactgcaaaa agcgttgctg tacttcttga atacaaagac aggaatgcaa
      601 gagaacttta cgagcaaaag tttgctcctg aaaaagctgt tccaataata gctataaact
      661 tgacacatgg tacaggtaca gaagttgata gatttgctgt tgcaactata cctgagaaaa
      721 attacaagcc tgcaattgca tatgattgta tctatccatt atactcaata gatgatcctc
      781 agcttatgac aaaacttgat aaaacacaaa ctgttgcagt tacagtagac gcgctcaatc
      841 acgttacaga agcctcaaca acattggtag catccccata cagtatttta accgcacagg
      901 agactgtaag gcttatagca aaatatctac ctgttgcaat aaatgagcca caaaacctta
      961 ttgcaaggta ttaccttttg tatgcatcag cattagcagg tatctcgttt gataacggac
     1021 ttttacatct tacacatgct cttgaacacc cgctaagtgc tgtaaagcct gaacttccac
     1081 atggcttggg acttggagca cttcttcctg ctatagtcaa gacaatttat ccatatcagg
     1141 ctgaagtttt ggcatctgtt tatgagccaa ttgttcctaa cttaaaagga gtgccttcag
     1201 aggcagaatt tgcagctaag aaagtggaag agtggttgtt tgaaatgggg tgcgataaga
     1261 aactttctga ctttggattt agccagagcg acgttgataa tcttgtggac ttggcacttg
     1321 agacaccgtc tttagggtta ctcctttcaa tgtctccagt caaggcaacc aaagaagtaa
     1381 ttaagaagat ttacgaagaa tcattgtacc caatgaagtg attaatagaa ataattttgt
     1441 ttaactttac aaacgggatt gacttttaaa aaaggattga ttctaatgaa gaaagcagac
     1501 aagtaagcct cctaaattca ctttagataa aaatttagga ggcatatcaa atgaacttta
     1561 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
     1621 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
     1681 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
     1741 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
     1801 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
     1861 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
     1921 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
     1981 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
     2041 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
     2101 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
     2161 ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg
     2221 tactttttac agtcggtttt ctaatgtcac tagggctcgc ctttgggaag tttgaaggtt
     2281 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2341 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2401 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2461 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2521 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2581 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2641 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2701 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2761 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2821 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2881 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2941 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3001 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3061 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3121 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3181 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa aagagtttga
     3241 ggaaaactta agaaaacggg aaactatttc ctgttgagaa tgtgaaatta aatgataaaa
     3301 agtgatataa tagggtcagg cgtacagaag cctgaccttt ttgcaaaatt gttttattgg
     3361 cgcggcgtat atatacagca tatatacaat gtaaatttat acaatcaaat tgttgataca
     3421 atcgaattat gcggctaaaa atttgctcgc gattgctgaa taaaagatac gagagacctc
     3481 tcttgtatct tttttatttt gagtggtttt gtccgttaca ctagaaaacc gaaagacaat
     3541 aaaaatttta ttcttgctga gtctggcttt cggtaagcta gacaaaacgg acaaaataaa
     3601 aattggcaag ggtttaaagg tggagatttt ttgagtgatc ttctcaaaaa atactacctg
     3661 tcccttgctg atttttaaac gagcacgaga gcaaaacccc cctttgctga ggtggcagag
     3721 ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg
     3781 ttttggtcat gagcctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt
     3841 tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt
     3901 tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag
     3961 tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc
     4021 tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga
     4081 ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
     4141 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
     4201 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     4261 cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4321 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     4381 gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc
     4441 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     4501 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc
     4561 cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa
     4621 gccagtatac aaatgagtgc tttttttgcg ttttgagcgt agcgaaaaac gagttctttc
     4681 tattcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
     4741 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
     4801 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
     4861 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
     4921 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
     4981 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
     5041 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
     5101 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
     5161 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
     5221 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
     5281 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc ttgttggttt
     5341 tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc agcacatgca
     5401 tgtattggta tgtgtggaac caacttattt taagaataca gaaaactacg tgaatcaaaa
     5461 acaatggatt caattttgga aaaaggcaat gaaattagac tatgatccaa atgtaaaagt
     5521 tcaaatgatt cgaccgaaaa ataaatataa atcggatata caatcggcaa ttgacgaaac
     5581 tgcaaaatat cctgtaaagg atacggattt tatgaccgat gatgaagaaa agaatttgaa
     5641 acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct atggtggttt
     5701 gttaaaagaa atacataaaa aattaaacct tgatgacaca gaagaaggcg atttgattca
     5761 tacagatgat gacgaaaaag ccgatgaaga tggattttct attattgcaa tgtggaattg
     5821 ggaacggaaa aattatttta ttaaagagta gttcaacccg gggatcctct agagtcgacc
     5881 tgcaggcatg caagct
//
