LOCUS       V008694                 5893 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008694
VERSION     V008694
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1418
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Fervidobacterium nodosum"
                     /protein_id="AUE21773.1"
                     /translation="MWERKVNIYNVFELRCKTTCYFGVGAIEKIKDIAEWLSSSKKDKV
                     LIVSDPVAYKVTGAWDKVEKALKERNIGYVLYDKVTPNPTTDQIDEATKIGRDFGAEAV
                     IGIGGGSPIDTAKSVAVLLEHKDKNARELYEGKFIPERAKPIIAINTTHGTGTEVDRFA
                     VASILEKEYKPAIAYDVIYPTFAIDDPQLMVTLPYKQTLYTSIDALNHITEAATTLARN
                     PYSILLAKETVRLIATYLPQALSNPNDLNARYYLLYASAIAGISFDNGLLHFTHALEHP
                     LSAVKPELAHGLGLAMLLPAVVKEIYPSTAEILAEVYEPIVPGLKGNPAEAEKIAKGIE
                     DWLFAMGVTQKLEDEGFKDSDIEKLTNLALTTPSLDLLLSVAPIKAEREVIARIYKNSL
                     RRM"
     gene            210..1418
                     /gene="adhA"
                     /label="adhA"
     CDS             1548..2195
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2279..3136)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3137..3228)
                     /label="AmpR promoter"
     rep_origin      3828..4373
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4844..5845
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgtgggaaag aaaagtcaac atttacaatg
      241 tttttgagct taggtgtaag acaacgtgtt actttggtgt tggtgcgatc gagaaaataa
      301 aagatattgc cgagtggctt tcttctagta aaaaagataa agttttgata gtttctgacc
      361 ctgttgcgta taaggtcaca ggggcatggg acaaagtgga aaaggcgctt aaggaaagga
      421 acattggata tgttttatac gataaggtaa cgccaaatcc gacaacggat caaattgatg
      481 aagcaacgaa aattggaaga gattttggag cagaagcggt tatcggtata ggtgggggaa
      541 gtcctattga tacggcaaaa agtgtagctg tgttacttga gcataaagat aagaatgcgc
      601 gagaacttta cgaaggaaag tttataccag agagggcaaa acctataatt gccatcaata
      661 caactcatgg tactggtaca gaggttgata gattcgccgt tgcgtcgata ttggagaagg
      721 aatataaacc agctattgcg tacgatgtta tttatccaac attcgccata gacgacccac
      781 aacttatggt tactctaccg tataagcaaa cgctatatac gtctattgat gcacttaatc
      841 atataacaga ggcagcaaca acactggcaa ggaatccata ttcgatactc cttgcaaaag
      901 aaactgttag attgattgcg acatatctgc cacaagcttt gagtaatccg aacgatttaa
      961 atgcaagata ctatttactc tatgcttcgg cgatagcagg tatatctttt gataatggac
     1021 ttttgcattt cacacatgct ttagaacatc ctttaagtgc tgttaaacct gagcttgctc
     1081 acggtttggg acttgctatg cttttaccag ctgttgttaa ggaaatttac ccatcaactg
     1141 cagaaattct tgctgaagtg tacgaaccta ttgtccctgg cttaaaaggt aatcctgcgg
     1201 aagctgagaa aattgcaaaa ggtatagagg actggttatt tgccatggga gttacacaaa
     1261 aacttgagga tgaaggattt aaagactcag atattgaaaa gcttaccaat cttgcgctta
     1321 caactccttc tcttgattta ttgctcagcg tggcacctat aaaggctgaa agggaagtta
     1381 tagcaagaat atacaagaat tctttgagaa gaatgtgatt aatagaaata attttgttta
     1441 actttacaaa cgggattgac ttttaaaaaa ggattgattc taatgaagaa agcagacaag
     1501 taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg aactttaata
     1561 aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa
     1621 cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag
     1681 aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata
     1741 cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc
     1801 cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa
     1861 agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt
     1921 cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc
     1981 catggacttc atttactggg tttaacttaa atatcaataa taatagtaat taccttctac
     2041 ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt
     2101 tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc
     2161 aggaattgtc agataggcct aatgactggc ttttataata tgagataatg ccgactgtac
     2221 tttttacagt cggttttcta atgtcactag ggctcgcctt tgggaagttt gaaggttacc
     2281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2581 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2881 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaaag agtttgagga
     3241 aaacttaaga aaacgggaaa ctatttcctg ttgagaatgt gaaattaaat gataaaaagt
     3301 gatataatag ggtcaggcgt acagaagcct gacctttttg caaaattgtt ttattggcgc
     3361 ggcgtatata tacagcatat atacaatgta aatttataca atcaaattgt tgatacaatc
     3421 gaattatgcg gctaaaaatt tgctcgcgat tgctgaataa aagatacgag agacctctct
     3481 tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa
     3541 aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat
     3601 tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc
     3661 cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc
     3721 aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt
     3781 tggtcatgag cctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt
     3841 ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg
     3901 aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca
     3961 ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct
     4021 aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc
     4081 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca
     4141 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
     4201 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     4261 gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     4321 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     4381 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     4441 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     4501 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     4561 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     4621 agtatacaaa tgagtgcttt ttttgcgttt tgagcgtagc gaaaaacgag ttctttctat
     4681 tcttgataca tatagaaata acgtcatttt tattttagtt gctgaaaggt gcgttgaagt
     4741 gttggtatgt atgtgtttta aagtattgaa aacccttaaa attggttgca cagaaaaacc
     4801 ccatctgtta aagttataag tgaccaaaca aataactaaa tagatggggg tttcttttaa
     4861 tattatgtgt cctaatagta gcatttattc agatgaaaaa tcaagggttt tagtggacaa
     4921 gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa atcgctaatg ttgattactt
     4981 tgaacttctg catattcttg aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat
     5041 attagagtat aaacaaaatc gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg
     5101 taaatccagg ctttgtccaa tgtgcaactg gaggagagca atgaaacatg gcattcagtc
     5161 acaaaaggtt gttgctgaag ttattaaaca aaagccaaca gttcgttggt tgtttctcac
     5221 attaacagtt aaaaatgttt atgatggcga agaattaaat aagagtttgt cagatatggc
     5281 tcaaggattt cgccgaatga tgcaatataa aaaaattaat aaaaatcttg ttggttttat
     5341 gcgtgcaacg gaagtgacaa taaataataa agataattct tataatcagc acatgcatgt
     5401 attggtatgt gtggaaccaa cttattttaa gaatacagaa aactacgtga atcaaaaaca
     5461 atggattcaa ttttggaaaa aggcaatgaa attagactat gatccaaatg taaaagttca
     5521 aatgattcga ccgaaaaata aatataaatc ggatatacaa tcggcaattg acgaaactgc
     5581 aaaatatcct gtaaaggata cggattttat gaccgatgat gaagaaaaga atttgaaacg
     5641 tttgtctgat ttggaggaag gtttacaccg taaaaggtta atctcctatg gtggtttgtt
     5701 aaaagaaata cataaaaaat taaaccttga tgacacagaa gaaggcgatt tgattcatac
     5761 agatgatgac gaaaaagccg atgaagatgg attttctatt attgcaatgt ggaattggga
     5821 acggaaaaat tattttatta aagagtagtt caacccgggg atcctctaga gtcgacctgc
     5881 aggcatgcaa gct
//
