LOCUS       V008695                 5884 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008695
VERSION     V008695
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5884)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1409
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Thermoanaerobacterium
                     thermosaccharolyticum"
                     /protein_id="AUE21771.1"
                     /translation="MWETKINPSKVFELRCKNTTYFGIGSIQKIKDILEVLKNKGIDNV
                     IFITGKGSYKASGAWDVVKPILDELDLKYSLYDRVGPNPTVDMIDEATKIGRESGAKAV
                     VGIGGGSPIDTAKSVAVLLKYTDKNARELYEQKFIPEEAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMYAIDDPALMTKLDKRQTIAVTVDALNHITEAATTLAAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNLVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEILADVYSPMVPGLKGLPSEAEYVSQKVE
                     EWLFNVGCTQKLTDFGFTKEDIPTLVNLAKTTPSLDGLLSIAPIEATESVIEKIYVESL
                     "
     gene            210..1409
                     /gene="adhA"
                     /label="adhA"
     CDS             1539..2186
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2270..3127)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3128..3219)
                     /label="AmpR promoter"
     rep_origin      3819..4364
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4835..5836
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gataaacaaa ggacggttca gggcttctgc tcatcctact ctgcattgta aaaaggtagg
       61 atgaattttt atttttaatc ttattgaaaa aaatttttga aaatcggttt tattaaaaaa
      121 aagtgggtat atttataata gtcaattgat tggttaaaaa aatttaaata agcaaacaga
      181 ataataacaa aagtaaggag gaatttgtta tgtgggaaac aaaaataaac cctagtaaag
      241 tttttgagct acgctgtaaa aatacgactt attttggaat aggtagtata caaaaaataa
      301 aagatatttt agaggttctc aaaaataaag gtattgataa tgttattttt ataacaggaa
      361 agggttctta taaagccagc ggtgcttggg atgttgtgaa acctatactg gatgagcttg
      421 atttaaaata ttcactttat gatagggttg gtcctaatcc tactgttgac atgattgatg
      481 aggcaacaaa aattggacgc gaatctggag caaaagcagt tgtgggaatt ggtggtggca
      541 gtcctataga tactgctaaa agtgtagctg tacttttaaa gtatactgat aaaaatgcaa
      601 gagaacttta tgagcagaaa tttatacctg aagaggctgt tcctatcata gctataaatc
      661 ttactcacgg cactggcacg gaagtagaca gatttgcagt agccacaata ccagaaaaaa
      721 attataaacc ggctattgct tatgattgtc tgtatcctat gtatgcaata gatgatcctg
      781 cacttatgac taaacttgat aaaaggcaga caatagcagt aactgtagat gcattaaacc
      841 atattactga agctgctaca acacttgctg catccccgta tagcattctt actgcaaaag
      901 agacagtaag attgatagtt cgctatcttc ctgctgctgt aaatgatcct ttaaatcttg
      961 tagcaaggta ttatttgctg tatgcctctg ctttggctgg tatttctttt gacaatggtc
     1021 ttttgcacct tactcatgct ttggagcatc ctttgagtgc tgtaaaacct gagatagctc
     1081 atggtcttgg acttggagca atattacctg cagtgataaa agcaatttat cctgctacag
     1141 cagaaatttt ggctgatgta tatagcccta tggtgcctgg cttgaagggt ttgccttctg
     1201 aagcagaata tgtatctcaa aaagttgaag aatggctctt taacgttgga tgtactcaaa
     1261 agctaacaga ttttggattt acaaaagaag atattccgac tttagttaat cttgcaaaaa
     1321 caacaccttc tctagatgga ctgctatcta tagcaccgat agaggctaca gagtcagtta
     1381 ttgaaaagat ttatgtggaa tccctttaat taatagaaat aattttgttt aactttacaa
     1441 acgggattga cttttaaaaa aggattgatt ctaatgaaga aagcagacaa gtaagcctcc
     1501 taaattcact ttagataaaa atttaggagg catatcaaat gaactttaat aaaattgatt
     1561 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
     1621 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
     1681 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
     1741 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
     1801 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
     1861 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
     1921 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
     1981 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
     2041 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
     2101 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
     2161 cagataggcc taatgactgg cttttataat atgagataat gccgactgta ctttttacag
     2221 tcggttttct aatgtcacta gggctcgcct ttgggaagtt tgaaggttac caatgcttaa
     2281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2581 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2881 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3181 gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgagg aaaacttaag
     3241 aaaacgggaa actatttcct gttgagaatg tgaaattaaa tgataaaaag tgatataata
     3301 gggtcaggcg tacagaagcc tgaccttttt gcaaaattgt tttattggcg cggcgtatat
     3361 atacagcata tatacaatgt aaatttatac aatcaaattg ttgatacaat cgaattatgc
     3421 ggctaaaaat ttgctcgcga ttgctgaata aaagatacga gagacctctc ttgtatcttt
     3481 tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa aaattttatt
     3541 cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa ttggcaaggg
     3601 tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc ccttgctgat
     3661 ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg caggtttttt
     3721 tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt ttggtcatga
     3781 gcctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     3841 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     3901 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     3961 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     4021 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     4081 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     4141 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     4201 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     4261 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     4321 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     4381 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     4441 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     4501 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     4561 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacaa
     4621 atgagtgctt tttttgcgtt ttgagcgtag cgaaaaacga gttctttcta ttcttgatac
     4681 atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg
     4741 tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt
     4801 aaagttataa gtgaccaaac aaataactaa atagatgggg gtttctttta atattatgtg
     4861 tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag
     4921 tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact ttgaacttct
     4981 gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta
     5041 taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag
     5101 gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt
     5161 tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt
     5221 taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt
     5281 tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac
     5341 ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg tattggtatg
     5401 tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca
     5461 attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg
     5521 accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc
     5581 tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga
     5641 tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat
     5701 acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga
     5761 cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa
     5821 ttattttatt aaagagtagt tcaacccggg gatcctctag agtcgacctg caggcatgca
     5881 agct
//
