LOCUS       40924_9221        5234 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBR-Control, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5234)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..41
                     /label=multiple cloning region
                     /note="multiple cloning region"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             280..1905
                     /label=CBRluc
                     /note="red-emitting variant of click beetle luciferase"
     polyA_signal    complement(1951..2072)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2228..2419
                     /regulatory_class="enhancer"
     rep_origin      2734
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2737..3325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3499..4356)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4357..4461)
                     /label=AmpR promoter
     rep_origin      4488..4943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5074..5122
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5136..5227
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggtaaagcg tgagaaaaat
      301 gtcatctatg gccctgagcc tctccatcct ttggaggatt tgactgccgg cgaaatgctg
      361 tttcgtgctc tccgcaagca ctctcatttg cctcaagcct tggtcgatgt ggtcggcgat
      421 gaatctttga gctacaagga gttttttgag gcaaccgtct tgctggctca gtccctccac
      481 aattgtggct acaagatgaa cgacgtcgtt agtatctgtg ctgaaaacaa tacccgtttc
      541 ttcattccag tcatcgccgc atggtatatc ggtatgatcg tggctccagt caacgagagc
      601 tacattcccg acgaactgtg taaagtcatg ggtatctcta agccacagat tgtcttcacc
      661 actaagaata ttctgaacaa agtcctggaa gtccaaagcc gcaccaactt tattaagcgt
      721 atcatcatct tggacactgt ggagaatatt cacggttgcg aatctttgcc taatttcatc
      781 tctcgctatt cagacggcaa catcgcaaac tttaaaccac tccacttcga ccctgtggaa
      841 caagttgcag ccattctgtg tagcagcggt actactggac tcccaaaggg agtcatgcag
      901 acccatcaaa acatttgcgt gcgtctgatc catgctctcg atccacgcta cggcactcag
      961 ctgattcctg gtgtcaccgt cttggtctac ttgcctttct tccatgcttt cggctttcat
     1021 attactttgg gttactttat ggtcggtctc cgcgtgatta tgttccgccg ttttgatcag
     1081 gaggctttct tgaaagccat ccaagattat gaagtccgca gtgtcatcaa cgtgcctagc
     1141 gtgatcctgt ttttgtctaa gagcccactc gtggacaagt acgacttgtc ttcactgcgt
     1201 gaattgtgtt gcggtgccgc tccactggct aaggaggtcg ctgaagtggc cgccaaacgc
     1261 ttgaatcttc cagggattcg ttgtggcttc ggcctcaccg aatctaccag tgcgattatc
     1321 cagactctcg gggatgagtt taagagcggc tctttgggcc gtgtcactcc actcatggct
     1381 gctaagatcg ctgatcgcga aactggtaag gctttgggcc cgaaccaagt gggcgagctg
     1441 tgtatcaaag gccctatggt gagcaagggt tatgtcaata acgttgaagc taccaaggag
     1501 gccatcgacg acgacggctg gttgcattct ggtgattttg gatattacga cgaagatgag
     1561 catttttacg tcgtggatcg ttacaaggag ctgatcaaat acaagggtag ccaggttgct
     1621 ccagctgagt tggaggagat tctgttgaaa aatccatgca ttcgcgatgt cgctgtggtc
     1681 ggcattcctg atctggaggc cggcgaactg ccttctgctt tcgttgtcaa gcagcctggt
     1741 acagaaatta ccgccaaaga agtgtatgat tacctggctg aacgtgtgag ccatactaag
     1801 tacttgcgtg gcggcgtgcg ttttgttgac tccatccctc gtaacgtaac aggcaaaatt
     1861 acccgcaagg agctgttgaa acaattgttg gtgaaggccg gcggttagta atctagagtc
     1921 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     1981 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     2041 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     2101 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     2161 gtaaaatcga taaggatctg aacgatggag cggagaatgg gcggaactgg gcggagttag
     2221 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     2281 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     2341 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     2401 ctgacacaca ttccacagcg gatccgtcga ccgatgccct tgagagcctt caacccagtc
     2461 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     2521 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     2581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     4501 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4561 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4621 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4681 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4741 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4801 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4861 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4921 caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg
     4981 gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa
     5041 tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat
     5101 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     5161 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     5221 tttctctatc gata
//
