LOCUS       40924_9216        4796 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBR-Basic, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4796)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1713
                     /codon_start=1
                     /label=CBRluc
                     /note="red-emitting variant of click beetle luciferase"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RYGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGTEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLVKAGG"
     polyA_signal    complement(1759..1880)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2296
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2299..2887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3061..3918)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3919..4023)
                     /label=AmpR promoter
     rep_origin      4050..4505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4636..4684
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4698..4789
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gtaaagcgtg agaaaaatgt catctatggc
      121 cctgagcctc tccatccttt ggaggatttg actgccggcg aaatgctgtt tcgtgctctc
      181 cgcaagcact ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc
      241 tacaaggagt tttttgaggc aaccgtcttg ctggctcagt ccctccacaa ttgtggctac
      301 aagatgaacg acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc
      361 atcgccgcat ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac
      421 gaactgtgta aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt
      481 ctgaacaaag tcctggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg
      541 gacactgtgg agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca
      601 gacggcaaca tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc
      661 attctgtgta gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac
      721 atttgcgtgc gtctgatcca tgctctcgat ccacgctacg gcactcagct gattcctggt
      781 gtcaccgtct tggtctactt gcctttcttc catgctttcg gctttcatat tactttgggt
      841 tactttatgg tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg
      901 aaagccatcc aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcctgttt
      961 ttgtctaaga gcccactcgt ggacaagtac gacttgtctt cactgcgtga attgtgttgc
     1021 ggtgccgctc cactggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca
     1081 gggattcgtt gtggcttcgg cctcaccgaa tctaccagtg cgattatcca gactctcggg
     1141 gatgagttta agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct
     1201 gatcgcgaaa ctggtaaggc tttgggcccg aaccaagtgg gcgagctgtg tatcaaaggc
     1261 cctatggtga gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac
     1321 gacggctggt tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc
     1381 gtggatcgtt acaaggagct gatcaaatac aagggtagcc aggttgctcc agctgagttg
     1441 gaggagattc tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat
     1501 ctggaggccg gcgaactgcc ttctgctttc gttgtcaagc agcctggtac agaaattacc
     1561 gccaaagaag tgtatgatta cctggctgaa cgtgtgagcc atactaagta cttgcgtggc
     1621 ggcgtgcgtt ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag
     1681 ctgttgaaac aattgttggt gaaggccggc ggttagtaat ctagagtcgg ggcggccggc
     1741 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1801 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1861 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1921 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     1981 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     2041 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     2101 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4021 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     4081 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4141 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4201 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4261 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4321 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4381 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4441 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4501 aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4561 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt
     4621 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     4681 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     4741 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata
//
