LOCUS       .                       7826 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        396..681
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV
                     enhancer"
     promoter        683..960
                     /label="chicken β-actin promoter"
     CDS             1210..1230
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3553..3573
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3583..3717
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        3724..3964
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        4114..4417
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4418..4621
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             4656..5363
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     polyA_signal    5383..5607
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6029..6133
                     /label="AmpR promoter"
     CDS             6134..6991
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      7165..7753
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      841 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     1021 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     1081 tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg ttggtggggt
     1141 attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg ttggaccggt
     1201 gccaccatgc ccaagaagaa gcggaaggtg gcccaggtga gcaagcagac ctccaagaag
     1261 agggagctga gcatcgacga gtaccagggc gcccggaagt ggtgcttcac cattgccttc
     1321 aacaaggccc tggtgaaccg ggacaagaac gacggcctgt tcgtggaaag cctgctgaga
     1381 cacgagaagt acagcaagca cgactggtac gacgaagata cccgggccct gatcaagtgc
     1441 agcacccagg ccgccaacgc caaggctgaa gccctgcgga actacttcag tcactaccgg
     1501 catagccctg gctgcctgac cttcaccgcc gaggacgaac tgcggaccat catggagaga
     1561 gcctatgagc gggccatctt cgagtgcaga agaagagaga cagaggtgat catcgagttt
     1621 cccagcctgt tcgagggcga ccggatcacc accgccggcg tggtgttttt cgtgagcttt
     1681 ttcgtggaaa gaagagtgct ggatcggctg tatggagccg tgtccggcct gaagaagaat
     1741 gagggacagt acaagctgac ccggaaggcc ctgagcatgt actgcctgaa ggacagcaga
     1801 ttcaccaagg cctgggataa gcgggtgctg ctgttcagag acatcctggc ccagctggga
     1861 agaatccccg ccgaggccta cgagtactac cacggcgagc agggtgataa gaagagagct
     1921 aacgacaatg agggcacaaa tcccaagcgg cacaaggaca agttcatcga atttgcactg
     1981 cactacctgg aagcccagca cagcgagatc tgcttcggca gacgccacat cgtgcgggaa
     2041 gaggccggcg ccggcgatga gcacaagaag caccggacca agggaaaggt ggtggtggac
     2101 ttcagcaaga aggacgagga ccagagctac tatatctcca agaacaacgt gatcgtgcgg
     2161 atcgacaaga acgccggccc tagaagctac cggatgggcc tgaacgagct gaagtacctc
     2221 gtgctgctga gcctgcaggg gaagggcgac gatgccatcg ccaagctgta cagatacaga
     2281 cagcacgtgg agaacatcct ggatgtggtg aaggtgaccg ataaggataa ccacgtgttc
     2341 ctgccccgct tcgtgctgga gcagcacggc atcggcagaa aggccttcaa gcagcggatc
     2401 gatggacggg tgaagcacgt gcggggcgtg tgggagaaga agaaggccgc caccaatgaa
     2461 atgaccctgc acgagaaggc cagagacatc ctgcagtacg tgaacgaaaa ctgcacccgg
     2521 tccttcaacc ctggcgaata caacagactg ctggtgtgcc tggtgggcaa ggacgtggag
     2581 aactttcagg ccggcctgaa gcggctgcag ctggccgaaa ggatcgatgg ccgggtgtac
     2641 tccatcttcg cccagaccag caccatcaat gagatgcacc aggtggtgtg cgaccagatc
     2701 ctgaaccggc tgtgcagaat cggcgaccag aagctgtacg attacgtggg actgggcaag
     2761 aaggacgaaa tcgactacaa gcagaaggtg gcctggttca aggagcacat cagcatccgg
     2821 agaggattcc tgagaaagaa gttctggtac gatagcaaga agggattcgc aaagctggtg
     2881 gaggaacacc tggagtccgg cggcggccag cgcgacgtgg gcctggacaa gaagtactac
     2941 cacatcgacg ccatcggcag attcgagggc gccaaccccg ccctgtacga gaccctggcc
     3001 agagatcggc tgtgcctcat gatggcccag tacttcctgg gcagcgtgag aaaggaactg
     3061 ggcaacaaga ttgtgtggag caacgacagc atcgaactgc ctgtggaagg ctctgtggga
     3121 aatgagaaga gcatcgtgtt ctccgtgtct gactacggca agctgtacgt gctggacgat
     3181 gccgaattcc tgggccggat ctgcgaatac ttcatgcccc acgaaaaggg caagatccgg
     3241 taccacacag tgtacgaaaa gggctttaga gcatacaacg acctgcagaa gaagtgcgtg
     3301 gaggccgtgc tggctttcga agagaaggtg gtgaaggcca agaagatgag cgagaaggaa
     3361 ggcgcccact acatcgactt ccgggagatc ctggcccaga ccatgtgcaa ggaggccgaa
     3421 aagaccgcag tgaacaaggt gagacgcgcc ttcttccacc accacctgaa gttcgtgatt
     3481 gacgagttcg gcctgttcag cgacgtgatg aagaagtacg gcatcgagaa ggaatggaag
     3541 ttccctgtca agcccaagaa gaagcggaag gtgtagaagc ttaacttgtt tattgcagct
     3601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcact
     3721 agtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     3781 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     3841 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     3901 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3961 ggacgaaaca ccggggtctt cgatattcaa gcgtcggaag acctgctgga gcagcccccg
     4021 atttgtgggg tgattacagc tttttttacg cgttagttat taatagtaat caattacggg
     4081 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4141 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4261 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4321 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4381 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     4441 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4501 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4561 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     4621 tggtttagtg aaccgtcaga tccgctagcg ccaccatggt gagcaagggc gaggaggata
     4681 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     4741 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     4801 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     4861 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     4921 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgat gacggcggcg
     4981 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5041 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaaaacc atgggctggg
     5101 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5161 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     5221 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     5281 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     5341 gcggcatgga cgagctgtac aagtaactct tggcccggat ccctgtgcct tctagttgcc
     5401 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5461 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5521 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaaaac aatagcaggc
     5581 atgctgggga tgcggtgggc tctatgggtc gacggcgcgc cctagacgaa gcagctccag
     5641 cctacacaat cgctcaagac gtgtaatgct tttattatat attagtcacg atatctataa
     5701 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     5761 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     5821 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     5881 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     5941 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     6001 aaggatccgc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7801 ttttgctcac atgttctttc ctgcgt
//
