LOCUS       40924_9206        5231 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBG99-Control, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5231)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..41
                     /label=multiple cloning region
                     /note="multiple cloning region"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             280..1905
                     /label=CBG99luc
                     /note="green-emitting variant of click beetle luciferase"
     polyA_signal    complement(1948..2069)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2225..2416
                     /regulatory_class="enhancer"
     rep_origin      2731
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2734..3322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3496..4353)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4354..4458)
                     /label=AmpR promoter
     rep_origin      4485..4940
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5071..5119
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5133..5224
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggtgaagcg tgagaaaaat
      301 gtcatctatg gccctgagcc tctccatcct ttggaggatt tgactgccgg cgaaatgctg
      361 tttcgtgctc tccgcaagca ctctcatttg cctcaagcct tggtcgatgt ggtcggcgat
      421 gaatctttga gctacaagga gttttttgag gcaaccgtct tgctggctca gtccctccac
      481 aattgtggct acaagatgaa cgacgtcgtt agtatctgtg ctgaaaacaa tacccgtttc
      541 ttcattccag tcatcgccgc atggtatatc ggtatgatcg tggctccagt caacgagagc
      601 tacattcccg acgaactgtg taaagtcatg ggtatctcta agccacagat tgtcttcacc
      661 actaagaata ttctgaacaa agtcctggaa gtccaaagcc gcaccaactt tattaagcgt
      721 atcatcatct tggacactgt ggagaatatt cacggttgcg aatctttgcc taatttcatc
      781 tctcgctatt cagacggcaa catcgcaaac tttaaaccac tccacttcga ccctgtggaa
      841 caagttgcag ccattctgtg tagcagcggt actactggac tcccaaaggg agtcatgcag
      901 acccatcaaa acatttgcgt gcgtctgatc catgctctcg atccacgcgt gggcactcag
      961 ctgattcctg gtgtcaccgt cttggtctac ttgcctttct tccatgcttt cggctttagc
     1021 attactttgg gttactttat ggtcggtctc cgcgtgatta tgttccgccg ttttgatcag
     1081 gaggctttct tgaaagccat ccaagattat gaagtccgca gtgtcatcaa cgtgcctagc
     1141 gtgatcctgt ttttgtctaa gagcccactc gtggacaagt acgacttgtc ttcactgcgt
     1201 gaattgtgtt gcggtgccgc tccactggct aaggaggtcg ctgaagtggc cgccaaacgc
     1261 ttgaatcttc cagggattcg ttgtggcttc ggcctcaccg aatctaccag cgctaacatt
     1321 cactctctcg gggatgagtt taagagcggc tctttgggcc gtgtcactcc actcatggct
     1381 gctaagatcg ctgatcgcga aactggtaag gctttgggcc cgaaccaagt gggcgagctg
     1441 tgtatcaaag gccctatggt gagcaagggt tatgtcaata acgttgaagc taccaaggag
     1501 gccatcgacg acgacggctg gttgcattct ggtgattttg gatattacga cgaagatgag
     1561 catttttacg tcgtggatcg ttacaaggag ctgatcaaat acaagggtag ccaggttgct
     1621 ccagctgagt tggaggagat tctgttgaaa aatccatgca ttcgcgatgt cgctgtggtc
     1681 ggcattcctg atctggaggc cggcgaactg ccttctgctt tcgttgtcaa gcagcctggt
     1741 aaagaaatta ccgccaaaga agtgtatgat tacctggctg aacgtgtgag ccatactaag
     1801 tacttgcgtg gcggcgtgcg ttttgttgac tccatccctc gtaacgtaac aggcaaaatt
     1861 acccgcaagg agctgttgaa acaattgttg gagaaggccg gcggttagtc tagagtcggg
     1921 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     1981 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2041 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2101 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     2161 aaatcgataa ggatctgaac gatggagcgg agaatgggcg gaactgggcg gagttagggg
     2221 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     2281 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     2341 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     2401 acacacattc cacagcggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     2461 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     2521 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     2581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     4501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4921 aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4981 atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat
     5041 taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc attacatctg
     5101 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     5161 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     5221 ctctatcgat a
//
