LOCUS       40924_9201        4793 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBG99-Basic, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1713
                     /codon_start=1
                     /label=CBG99luc
                     /note="green-emitting variant of click beetle luciferase"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     polyA_signal    complement(1756..1877)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2293
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2296..2884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3058..3915)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3916..4020)
                     /label=AmpR promoter
     rep_origin      4047..4502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4633..4681
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4695..4786
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gtgaagcgtg agaaaaatgt catctatggc
      121 cctgagcctc tccatccttt ggaggatttg actgccggcg aaatgctgtt tcgtgctctc
      181 cgcaagcact ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc
      241 tacaaggagt tttttgaggc aaccgtcttg ctggctcagt ccctccacaa ttgtggctac
      301 aagatgaacg acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc
      361 atcgccgcat ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac
      421 gaactgtgta aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt
      481 ctgaacaaag tcctggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg
      541 gacactgtgg agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca
      601 gacggcaaca tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc
      661 attctgtgta gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac
      721 atttgcgtgc gtctgatcca tgctctcgat ccacgcgtgg gcactcagct gattcctggt
      781 gtcaccgtct tggtctactt gcctttcttc catgctttcg gctttagcat tactttgggt
      841 tactttatgg tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg
      901 aaagccatcc aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcctgttt
      961 ttgtctaaga gcccactcgt ggacaagtac gacttgtctt cactgcgtga attgtgttgc
     1021 ggtgccgctc cactggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca
     1081 gggattcgtt gtggcttcgg cctcaccgaa tctaccagcg ctaacattca ctctctcggg
     1141 gatgagttta agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct
     1201 gatcgcgaaa ctggtaaggc tttgggcccg aaccaagtgg gcgagctgtg tatcaaaggc
     1261 cctatggtga gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac
     1321 gacggctggt tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc
     1381 gtggatcgtt acaaggagct gatcaaatac aagggtagcc aggttgctcc agctgagttg
     1441 gaggagattc tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat
     1501 ctggaggccg gcgaactgcc ttctgctttc gttgtcaagc agcctggtaa agaaattacc
     1561 gccaaagaag tgtatgatta cctggctgaa cgtgtgagcc atactaagta cttgcgtggc
     1621 ggcgtgcgtt ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag
     1681 ctgttgaaac aattgttgga gaaggccggc ggttagtcta gagtcggggc ggccggccgc
     1741 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1801 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1861 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1921 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     1981 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     2041 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     2101 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2281 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2461 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2701 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4021 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     4081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4501 ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4561 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
     4621 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     4681 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     4741 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
