LOCUS       40924_9196        5248 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBG68-Control, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5248)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..41
                     /label=multiple cloning region
                     /note="multiple cloning region"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             280..1905
                     /label=CBG68luc
                     /note="green-emitting variant of click beetle luciferase"
     polyA_signal    complement(1965..2086)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2242..2433
                     /regulatory_class="enhancer"
     rep_origin      2748
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2751..3339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3513..4370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4371..4475)
                     /label=AmpR promoter
     rep_origin      4502..4957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5088..5136
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5150..5241
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggtgaaacg cgaaaagaac
      301 gtgatctacg gcccagaacc actgcatcca ctggaagacc tcaccgctgg tgagatgctc
      361 ttccgagcac tgcgtaaaca tagtcacctc cctcaagcac tcgtggacgt cgtgggagac
      421 gagagcctct cctacaaaga atttttcgaa gctactgtgc tgttggccca aagcctccat
      481 aattgtgggt acaaaatgaa cgatgtggtg agcatttgtg ctgagaataa cactcgcttc
      541 tttattcctg taatcgctgc ttggtacatc ggcatgattg tcgcccctgt gaatgaatct
      601 tacatcccag atgagctgtg taaggttatg ggtattagca aacctcaaat cgtctttact
      661 accaaaaaca tcttgaataa ggtcttggaa gtccagtctc gtactaactt catcaaacgc
      721 atcattattc tggataccgt cgaaaacatc cacggctgtg agagcctccc taacttcatc
      781 tctcgttaca gcgatggtaa tatcgctaat ttcaagccct tgcattttga tccagtcgag
      841 caagtggccg ctattttgtg ctcctccggc accactggtt tgcctaaagg tgtcatgcag
      901 actcaccaga atatctgtgt gcgtttgatc cacgctctcg accctcgtgt gggtactcaa
      961 ttgatccctg gcgtgactgt gctggtgtat ctgcctttct ttcacgcctt tggtttctct
     1021 attaccctgg gctatttcat ggtcggcttg cgtgtcatca tgtttcgtcg cttcgaccaa
     1081 gaagccttct tgaaggctat tcaagactac gaggtgcgtt ccgtgatcaa cgtcccttca
     1141 gtcattttgt tcctgagcaa atctcctttg gttgacaagt atgatctgag cagcttgcgt
     1201 gagctgtgct gtggcgctgc tcctttggcc aaagaagtgg ccgaggtcgc tgctaagcgt
     1261 ctgaacctcc ctggtatccg ctgcggtttt ggtttgactg agagcacttc tgctaacatc
     1321 catagcttgc gagacgagtt taagtctggt agcctgggtc gcgtgactcc tcttatggct
     1381 gcaaagatcg ccgaccgtga gaccggcaaa gcactgggcc caaatcaagt cggtgaattg
     1441 tgtattaagg gccctatggt ctctaaaggc tacgtgaaca atgtggaggc cactaaagaa
     1501 gccattgatg atgatggctg gctccatagc ggcgacttcg gttactatga tgaggacgaa
     1561 cacttctatg tggtcgatcg ctacaaagaa ttgattaagt acaaaggctc tcaagtcgca
     1621 ccagccgaac tggaagaaat tttgctgaag aacccttgta tccgcgacgt ggccgtcgtg
     1681 ggtatcccag acttggaagc tggcgagttg cctagcgcct ttgtggtgaa acaacccggc
     1741 aaggagatca ctgctaagga ggtctacgac tatttggccg agcgcgtgtc tcacaccaaa
     1801 tatctgcgtg gcggcgtccg cttcgtcgat tctattccac gcaacgttac cggtaagatc
     1861 actcgtaaag agttgctgaa gcaactcctc gaaaaagctg gcggctagta aagtcttcat
     1921 gattatctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2161 cctctacaaa tgtggtaaaa tcgataagga tctgaacgat ggagcggaga atgggcggaa
     2221 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     2281 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     2341 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2401 tttccacacc ctaactgaca cacattccac agcggatccg tcgaccgatg cccttgagag
     2461 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2521 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2821 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2881 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2941 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3001 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3061 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3361 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4501 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4861 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4921 aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc
     4981 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     5041 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     5101 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     5161 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     5221 caggtgccag aacatttctc tatcgata
//
