LOCUS       40924_9191        4810 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Luciferase reporter vector pCBG68-Basic, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Chroma-Luc? Series Reporter Vectors Technical Manual #TM061
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Almond B, Chauvin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4810)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4810)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1713
                     /codon_start=1
                     /label=CBG68luc
                     /note="green-emitting variant of click beetle luciferase"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLRDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     polyA_signal    complement(1773..1894)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2310
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2313..2901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3075..3932)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3933..4037)
                     /label=AmpR promoter
     rep_origin      4064..4519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4650..4698
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4712..4803
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gtgaaacgcg aaaagaacgt gatctacggc
      121 ccagaaccac tgcatccact ggaagacctc accgctggtg agatgctctt ccgagcactg
      181 cgtaaacata gtcacctccc tcaagcactc gtggacgtcg tgggagacga gagcctctcc
      241 tacaaagaat ttttcgaagc tactgtgctg ttggcccaaa gcctccataa ttgtgggtac
      301 aaaatgaacg atgtggtgag catttgtgct gagaataaca ctcgcttctt tattcctgta
      361 atcgctgctt ggtacatcgg catgattgtc gcccctgtga atgaatctta catcccagat
      421 gagctgtgta aggttatggg tattagcaaa cctcaaatcg tctttactac caaaaacatc
      481 ttgaataagg tcttggaagt ccagtctcgt actaacttca tcaaacgcat cattattctg
      541 gataccgtcg aaaacatcca cggctgtgag agcctcccta acttcatctc tcgttacagc
      601 gatggtaata tcgctaattt caagcccttg cattttgatc cagtcgagca agtggccgct
      661 attttgtgct cctccggcac cactggtttg cctaaaggtg tcatgcagac tcaccagaat
      721 atctgtgtgc gtttgatcca cgctctcgac cctcgtgtgg gtactcaatt gatccctggc
      781 gtgactgtgc tggtgtatct gcctttcttt cacgcctttg gtttctctat taccctgggc
      841 tatttcatgg tcggcttgcg tgtcatcatg tttcgtcgct tcgaccaaga agccttcttg
      901 aaggctattc aagactacga ggtgcgttcc gtgatcaacg tcccttcagt cattttgttc
      961 ctgagcaaat ctcctttggt tgacaagtat gatctgagca gcttgcgtga gctgtgctgt
     1021 ggcgctgctc ctttggccaa agaagtggcc gaggtcgctg ctaagcgtct gaacctccct
     1081 ggtatccgct gcggttttgg tttgactgag agcacttctg ctaacatcca tagcttgcga
     1141 gacgagttta agtctggtag cctgggtcgc gtgactcctc ttatggctgc aaagatcgcc
     1201 gaccgtgaga ccggcaaagc actgggccca aatcaagtcg gtgaattgtg tattaagggc
     1261 cctatggtct ctaaaggcta cgtgaacaat gtggaggcca ctaaagaagc cattgatgat
     1321 gatggctggc tccatagcgg cgacttcggt tactatgatg aggacgaaca cttctatgtg
     1381 gtcgatcgct acaaagaatt gattaagtac aaaggctctc aagtcgcacc agccgaactg
     1441 gaagaaattt tgctgaagaa cccttgtatc cgcgacgtgg ccgtcgtggg tatcccagac
     1501 ttggaagctg gcgagttgcc tagcgccttt gtggtgaaac aacccggcaa ggagatcact
     1561 gctaaggagg tctacgacta tttggccgag cgcgtgtctc acaccaaata tctgcgtggc
     1621 ggcgtccgct tcgtcgattc tattccacgc aacgttaccg gtaagatcac tcgtaaagag
     1681 ttgctgaagc aactcctcga aaaagctggc ggctagtaaa gtcttcatga ttatctagag
     1741 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     1801 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1861 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1921 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1981 tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     2041 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     2101 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     2161 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2221 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2281 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2341 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2401 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2461 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2521 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2581 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2641 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2701 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2761 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2821 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2881 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2941 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3001 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3061 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     4081 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4141 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4201 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4261 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4321 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4381 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4441 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4501 atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     4561 tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt
     4621 aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca ttacatctgt
     4681 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     4741 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     4801 tctatcgata
//
