LOCUS       Exported               11446 bp DNA     circular SYN 13-SEP-2024
DEFINITION  Exported.
ACCESSION   V017331
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11446)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          279..563
                     /mol_type="other DNA"
                     /label=broad host range vector
                     /note="broad host range vector"
                     /db_xref="taxon:1265608"
                     /organism="Shuttle vector pKV12"
     source          join(564..11446,1..278)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          899..1228
                     /mol_type="other DNA"
                     /label=broad host range vector
                     /note="broad host range vector"
                     /db_xref="taxon:1265608"
                     /organism="Shuttle vector pKV12"
     gene            279..563
                     /gene="copS"
                     /label=copS
     CDS             279..563
                     /codon_start=1
                     /transl_table=11
                     /gene="copS"
                     /product="copy number repressor"
                     /label=copS
                     /protein_id="AFY24252.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTIK
                     TLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            899..1228
                     /gene="repD"
                     /label=repD
     CDS             899..1228
                     /codon_start=1
                     /transl_table=11
                     /gene="repD"
                     /product="replication protein RepD"
                     /label=repD
                     /protein_id="AFY24251.1"
                     /translation="MYSFIEGGVCMNHDECKTYIKNSLLEIRKLANIYTLETFKKELEK
                     RNIYLETKSDKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEK
                     ANQVN"
     CDS             1271..2761
                     /codon_start=1
                     /label=RepR
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGLTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     misc_RNA        complement(3487..3565)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             3861..7964
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     rep_origin      8509..9097
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9355..9373
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9942..9977
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes
                     CRISPR/Cas system"
     CDS             10662..11396
                     /gene="ermBP"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 ggaatgtaga taaattatta ggtatactac tgacagcttc caaggagcta aagaggtccc
       61 tagcgcttag aatcgcttta ggaaacacga tccagtccaa taatcgtcga taaaaacttt
      121 tgaaaaaggt tggtgaaatt acctactttt ggaataatca caaatcacaa gtgattaatc
      181 acaaatcaca agtgattaat cacttgttta ttaagatatt aaaagctata atttaaataa
      241 agcgtgaatt ttattacaca aaaagagggg ggagaaactt ggaactagca tttagagaaa
      301 gcttaaaaaa gatgagaggt accaaatcaa aagaaaaatt ctcccaagaa ttagaaatga
      361 gtagatcaaa ttattcacga atagaatcag gaaaatcaga tccaaccata aaaacactag
      421 aacaaattgc aaagttaact aactcaacgc tagtagtgga tttaatccca aatgagccaa
      481 cagaaccaga accagaaaca gaatcagaac aagtaacatt ggatttagaa atggaagaag
      541 aaaaaagcaa tgacttcgtg tgaataatgc acgaaatcgt tgcttatttt ttttaaaagc
      601 ggtatactag atataacgaa acaacgaact gaatagaaac gaaaaaagag ccatgacaca
      661 tttataaaat gtttgacgac attttataaa tgcatagccc gataagattg ccaaaccaac
      721 gcttatcagt tagtcagatg aactcttccc tcgtaagaag ttatttaatt aactttgttt
      781 gaagacggta tataaccgta ctatcattat atagggaaat cagagagttt tcaagtatct
      841 aagctactga atttaagaat tgttaagcaa tcaatcggaa atcgtttgat tgcttttttt
      901 gtattcattt atagaaggtg gagtttgtat gaatcatgat gaatgtaaaa cttatataaa
      961 aaatagttta ttggagataa gaaaattagc aaatatctat acactagaaa cgtttaagaa
     1021 agagttagaa aagagaaata tctacttaga aacaaaatca gataagtatt tttcttcgga
     1081 gggggaagat tatatatata agttaataga aaataacaaa ataatttatt cgattagtgg
     1141 aaaaaaattg acttataaag gaaaaaaatc tttttcaaaa catgcaatat tgaaacagtt
     1201 gaatgaaaaa gcaaaccaag ttaattaaac aacctatttt ataggattta taggaaagga
     1261 gaacagctga atgaatatcc cttttgttgt agaaactgtg cttcatgacg gcttgttaaa
     1321 gtacaaattt aaaaatagta aaattcgctc aatcactacc aagccaggta aaagcaaagg
     1381 ggccattttt gcgtatcgct caaaatcaag catgattggc ggtcgtggtg ttgttctgac
     1441 ttccgaggaa gcgattcaag aaaatcaaga tacatttaca cattggacac ccaacgttta
     1501 tcgttatgga acgtatgcag acgaaaaccg ttcatacacg aaaggacatt ctgaaaacaa
     1561 tttaagacaa atcaatacct tctttattga ttttgatatt cacacggcaa aagaaactat
     1621 ttcagcaagc gatattttaa caaccgctat tgatttaggt tttatgccta ctatgattat
     1681 caaatctgat aaaggttatc aagcatattt tgttttagaa acgccagtct atgtgacttc
     1741 aaaatcagaa tttaaatctg tcaaagcagc caaaataatt tcgcaaaata tccgagaata
     1801 ttttggaaag tctttgccag ttgatctaac gtgtaatcat tttggtattg ctcgcatacc
     1861 aagaacggac aatgtagaat tttttgatcc taattaccgt tattctttca aagaatggca
     1921 agattggtct ttcaaacaaa cagataataa gggccttact cgttcaagtc taacggtttt
     1981 aagcggtaca gaaggcaaaa aacaagtaga tgaaccctgg tttaatctct tattgcacga
     2041 aacgaaattt tcaggagaaa agggtttaat agggcgtaat aacgtcatgt ttaccctctc
     2101 tttagcctac tttagttcag gctattcaat cgaaacgtgc gaatataata tgtttgagtt
     2161 taataatcga ttagatcaac ccttagaaga aaaagaagta atcaaaattg ttagaagtgc
     2221 ctattcagaa aactatcaag gggctaatag ggaatacatt accattcttt gcaaagcttg
     2281 ggtatcaagt gatttaacca gtaaagattt atttgtccgt caagggtggt ttaaattcaa
     2341 gaaaaaaaga agcgaacgtc aacgtgttca tttgtcagaa tggaaagaag atttaatggc
     2401 ttatattagc gaaaaaagcg atgtatacaa gccttattta gtgacgacca aaaaagagat
     2461 tagagaagtg ctaggcattc ctgaacggac attagataaa ttgctgaagg tactgaaggc
     2521 gaatcaggaa attttcttta agatcaaacc aggaagaaat ggtggcattc aacttgctag
     2581 tgttaaatca ttgttgctat cgatcattaa agtaaaaaaa gaagaaaaag aaagctatat
     2641 aaaggcgctg acaaattctt ttgacttaga gcatacattc attcaagaga ctttaaacaa
     2701 gctagcagaa cgccctaaaa cggacacaca actcgatttg tttagctatg atacaggctg
     2761 aaaataaaac ccgcactatg ccattacatt tatatctatg atacgtgttt gttttttctt
     2821 tgctgtttag cgaatgatta gcagaaatat acagagtaag attttaatta attattaggg
     2881 ggagaaggag agagtagccc ggaaactttt agttggcttg gactgaacga agtgagggaa
     2941 aggctactaa aacgtcgagg ggcagtgaga gcgaagcgaa cacttgattt tttaattttc
     3001 tatcttttat aggtcattag agtatactta tttgtcctat aaactattta gcagcataat
     3061 agatttattg aataggtcat ttaagttgag catattagag gaggaaaatc ttggagaaat
     3121 atttgaagaa cccgattaca tggattggat tagttcttgt ggttacgtgg tttttaacta
     3181 aaagtagtga atttttgatt tttggtgtgt gtgtcttgtt gttagtattt gctagtcaaa
     3241 gtgattaaat agaattctca tgtttgacag cttatcatcg gagctctgat gataagcggt
     3301 ccaaacatga gaattcctta cgaaatcatc ctgtggagct tagtaggttt agcaagatgg
     3361 cagcgcctaa atgtagaatg ataaaaggat taagagatta atttccctaa aaatgataaa
     3421 acaagcgttt tgaaagcgct tgtttttttg gtttgcagtc agagtagaat agaagtatca
     3481 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
     3541 ttgctatgct gttttgaatg gttccaacaa gattatttta taacttttat aacaaataat
     3601 caaggagaaa ttcaaagaaa tttatcagcc ataaaacaat acttaatact atagaatgat
     3661 aacaaaataa actacttttt aaaagaattt tgtgttataa tctatttatt attaagtatt
     3721 gggtaatatt ttttgaagag atattttgaa aaagaaaaat taaagcatat taaactaatt
     3781 tcggaggtca ttaaaactat tattgaaatc atcaaactca ttatggattt aatttaaact
     3841 ttttatttta ggaggcaaaa atggataaga aatactcaat aggcttagat atcggcacaa
     3901 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
     3961 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
     4021 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
     4081 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
     4141 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
     4201 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
     4261 ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa
     4321 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt
     4381 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc
     4441 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg
     4501 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga
     4561 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat
     4621 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg
     4681 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta
     4741 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta
     4801 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc
     4861 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc
     4921 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata
     4981 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa
     5041 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa
     5101 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa
     5161 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc
     5221 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta
     5281 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac
     5341 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc
     5401 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa
     5461 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca
     5521 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat
     5581 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct
     5641 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag
     5701 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg
     5761 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc
     5821 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc
     5881 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta
     5941 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt
     6001 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta
     6061 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc
     6121 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc
     6181 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc
     6241 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct
     6301 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa
     6361 gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca
     6421 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag
     6481 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc
     6541 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag
     6601 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa
     6661 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta
     6721 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata
     6781 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg
     6841 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata
     6901 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg
     6961 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa
     7021 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct
     7081 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata
     7141 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa
     7201 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt
     7261 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat
     7321 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct
     7381 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact
     7441 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc
     7501 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga
     7561 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac
     7621 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca
     7681 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat
     7741 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta
     7801 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta
     7861 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg
     7921 gtctttatga aacacgtatt gatttgagtc agctaggagg tgactgaaaa aggtggggca
     7981 gaagttatcc taaaaataaa aaagtaattt agtaatctct aaggattact ttttttgttt
     8041 cggcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8101 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8161 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8221 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8281 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8701 agcaccgcct acatacctcg ctttgctaat cctgttacca gtggctgctg ccagtggcga
     8761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9181 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9241 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9301 tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg aaattaatac
     9361 gactcactat agggagaccg gcctcgagaa gctcgcgtta tcggtccttt aattggtgga
     9421 caaatttaca ttagtctcgg acattctgct cccgccctta tgggatttat cttccttatt
     9481 ctcgctttga ttgttctatc gaaagcgaaa tcaaacgatt aatttataaa taaaaaaacc
     9541 acctctaaaa ggtgattttt atttataaat tacaattttt cagcaatatc agtaattgct
     9601 ttatcaactg ctgctttttg gctatcaatc aaagcaacac gtgctgtaat ttgtttaatt
     9661 gccatttcaa ttgaacgttt caagccttgg ttttctaatt ttggttcaaa gaaagcttga
     9721 gctctcgagt caggggtacc gatcaggaaa ttaaaattgg tctcatataa ctgaattatt
     9781 ttcggaaaat aaagggaatc tgtttacaaa cattaccagt atcgatataa tacttaagga
     9841 ttcttcgaaa attgactact ttgtcttttc cagaagatgt agtataataa cacttagaaa
     9901 tgcattgatg cgaaattgat gtaattcttg aaaacatgta cgttttagag ctatgctgtt
     9961 ttgaatggtc ccaaaacttc gatcgacgaa cgtgtcacta aagccttgtt ttagagctat
    10021 gctgttttga atggtcccaa aacatgcggc cgcgccacac cgcaaacccc gcccctgaca
    10081 gggcggggtt tcgccgcgat caactcattg cagacatttg cagtacccat ggtgagtgcc
    10141 tccttataat ttattttgta gttccttcga acgaaatcat tgtatctaac aaacttcaga
    10201 atttaatcag agccgtttat tatgctcgcg ttatcgacaa taatattatt accaatactt
    10261 tctcaagata gaattaagac tgttttagat ttgttaatgt ttctattgtc agtatagtta
    10321 taagactaga tctgatccgt agcggttttc aaaatttgca accaggaatg aattactatc
    10381 ccttttatca agaagcgcac aaaaagaaaa acgaaatgat acaccaatca gtgcaaaaaa
    10441 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat
    10501 cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac ttagaagcaa
    10561 acttaagagt gtgttgatag tgcagtatct taaaattttg tataatagga attgaagtta
    10621 aattagatgc taaaaatttg taattaagaa ggagtgatta catgaacaaa aatataaaat
    10681 attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt
    10741 taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac
    10801 tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat
    10861 cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt
    10921 ttcaattccc taacaaacag aggtataaaa ttgttgggag tattccttac catttaagca
    10981 cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat ctgattgttg
    11041 aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca
    11101 ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa
    11161 aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt
    11221 ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta
    11281 ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta
    11341 cttatgagca agtattgtct atttttaata gttatctatt atttaacggg aggaaataat
    11401 tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaag
//
