LOCUS       40924_9101        7758 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCB112-HPV11-L1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson AL.
  TITLE     Evaluation of recombinant BCG expressing rotavirus VP6 as an 
            anti-rotavirus vaccine
  JOURNAL   Vaccine 25 (18), 3646-3657 (2007)
  PUBMED    17339069
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Pupa D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 7758)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine"; 
            date: "2007"; volume: "25"; issue: "18"; pages: "3646-3657"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-AUG-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      163..168
                     /label=BCG hsp60 promoter
                     /note="BCG hsp60 promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      186..191
                     /label=BCG hsp60 promoter
                     /note="BCG hsp60 promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    198
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      364..370
                     /regulatory_class="ribosome_binding_site"
     CDS             382..1749
                     /codon_start=1
                     /product="BCG hsp60-lysA chimaera"
                     /label=BCG hsp60-lysA chimaera
                     /protein_id="ABB00292.1"
                     /translation="MAKTIADPDVNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQ
                     EYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTG
                     GELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGI
                     VQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSH
                     IGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPI
                     AELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRR
                     YVSVDGGMSDNIRTGLYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPTI
                     FGPAIWLRLPPPALLLFAVESLQHGRPSRCGSGARGNARLVLRRETVDDLLSLEVR"
     CDS             1877..2689
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3771..5666
                     /note="Ori M region: mycobacterial origin of replication
                     derived from pAL5000 presented in Genbank Accession Number 
                     M60875"
     regulatory      5873..5878
                     /label=Mycobacterium leprae 18 kDa promoter
                     /note="Mycobacterium leprae 18 kDa promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      5896..5901
                     /label=Mycobacterium leprae 18 kDa promoter
                     /note="Mycobacterium leprae 18 kDa promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    5910
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      5960..5966
                     /regulatory_class="ribosome_binding_site"
     CDS             5975..7615
                     /codon_start=1
                     /product="BCG alpha antigen-HPV L1 chimaera"
                     /label=BCG alpha antigen-HPV L1 chimaera
                     /protein_id="ABB00294.1"
                     /translation="MATDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFGGP
                     MWRPSDSTVYVPPPNPVSKVVATDAYVKRTNIFYHASSSRLLAVGHPYYSIKKVNKTVV
                     PKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHP
                     LLNKYDDVENSGGYGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGTQCSNTSV
                     QNGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPLDICGTVCKYPDYLQMAA
                     DPYGDRLFFYLRKEQMFARHFFNRAGTVGEPVPDDLLVKGGNNRSSVASSIYVHTPSGS
                     LVSSEAQLFNKPYWLQKAQGHNNGICWGNHLFVTVVDTTRSTNMTLCASVSKSATYTNS
                     DYKEYMRHVEEFDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDT
                     YRYVQSQAITCQKPTPEKEKQDPYKDMSFWEVNLKEKFSSELDQFPLGRKFLLQSGYRG
                     RTSARTGIKRPAVSKPSTAPKRKRTKTKK"
     terminator      7676..7722
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccaag acaattgcgg atccagatgt gaacgagctg
      421 ctgcacttag cgccgaatgt gtggccgcgc aatactactc gcgatgaagt cggtgtggtc
      481 tgcatcgcag gaattccact gacgcagctc gcccaggagt acgggacccc gctgttcgtc
      541 atcgacgagg acgactttcg ctcgcgctgc cgagaaaccg ccgcggcctt tggaagtggg
      601 gcgaacgtgc actatgccgc caaggcgttc ctgtgcagcg aagtagcccg gtggatcagc
      661 gaagaagggc tctgtctgga cgtttgcacc ggtggggagt tggcggtcgc gctgcacgct
      721 agctttccgc ccgagcgaat taccttgcac ggcaacaaca aatcggtctc agagttgacc
      781 gctgcggtca aagccggagt cggccatatt gtcgtcgatt cgatgaccga gatcgagcgc
      841 ctcgacgcca tcgcgggcga ggccggaatc gtccaggatg tcctggtgcg tctcaccgtc
      901 ggtgtcgagg cgcacaccca cgagttcatc tccaccgcgc acgaggacca gaaattcggg
      961 ttatcggtgg ccagcggcgc ggccatggca gcggtgcggc gcgttttcgc cactgatcac
     1021 ctgcgcctgg ttgggctaca cagccacatc ggttcgcaga tcttcgacgt ggacggcttc
     1081 gaactcgccg cgcaccgtgt catcggcctg ctacgcgacg tcgtcggcga gttcggtccc
     1141 gaaaagacgg cacagatcgc gaccgtcgat ctcggtggcg gcttgggcat ctcgtatttg
     1201 ccgtccgacg acccaccgcc gatagccgag ctcgcggcca agctgggtac catcgtgagc
     1261 gacgagtcaa cggccgtggg gctgccgacg cccaagctcg ttgtggagcc cggacgcgcc
     1321 atcgccggac cgggcaccat cacgttgtat gaggtcggca ccgttaagga cgtcgatgtc
     1381 agcgccacag cgcatcgacg ttacgtcagt gtcgacggcg gcatgagcga caacatccgc
     1441 accggcctct acggcgcgca gtatgacgtc cggctggtgt ctcgagtcag cgacgccccg
     1501 ccggtaccgg cccgtctggt cggaaagcac tgcgaaagtg gcgatatcat cgtgcgggac
     1561 acctgggtgc cgacgatatt cggcccggcg atctggttgc ggttgccgcc accggcctta
     1621 ctgctattcg ctgtcgagtc gttacaacat ggtcggccgt cccgctgtgg tagcggtgca
     1681 cgcggcaacg ctcgcctggt cctgcgtcgg gagacggtcg acgatttgct gagtttggaa
     1741 gtgaggtgaa ctagtctcta gccaacaaag cgacgttgtg tctcaaaatc tctgatgtta
     1801 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1861 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1981 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa
     2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2161 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2401 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac
     2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2521 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2581 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2641 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2701 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     2761 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     2821 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     2881 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     2941 gagtcagcaa caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac
     3781 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc
     3841 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc
     3901 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc
     3961 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg
     4021 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt
     4081 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac
     4141 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc
     4201 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac
     4261 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc
     4321 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc
     4381 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc
     4441 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac
     4501 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac
     4561 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac
     4621 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg
     4681 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc
     4741 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt
     4801 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc
     4861 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg
     4921 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg
     4981 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg
     5041 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg
     5101 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg
     5161 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg
     5221 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
     5281 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
     5341 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
     5401 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
     5461 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa
     5521 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
     5581 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
     5641 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagaata
     5701 gcccgcagcg ttcatagagc cgcagcgacg gcaccgggaa cgccggaaag ttcggccgct
     5761 accgatgatg tcgtatacgc tgcgttgcag tgccgacgta cccgtgccgg cactacaatc
     5821 ggtcatgagc aatctcctca gctgttcaga cagaaaactt gtctatcaca acttgcatca
     5881 atatatcgac cagtgctata tcaaatctat gtagtcagga acagctatat agttatagtt
     5941 tgtcacaaca gattggagtg cgaggtgacc acatatggcc acagacgtga gccgaaagat
     6001 tcgagcttgg ggacgccgat tgatgatcgg cacggcagcg gctgtagtcc ttccgggcct
     6061 ggtggggctt gccggcggag cggcaaccgc gggcgcgttc ggtgggccca tgtggcggcc
     6121 tagtgacagc acagtatatg tgcctcctcc caaccctgta tccaaggttg ttgccacgga
     6181 tgcgtatgtt aaacgcacca acatatttta tcatgccagc agttctagac tccttgctgt
     6241 gggacatcca tattactcta tcaaaaaagt taacaaaaca gttgtaccaa aggtgtctgg
     6301 atatcaatat agagtgttta aggtagtgtt gccagatcct aacaagtttg cattacctga
     6361 ttcatctctg tttgacccca ctacacagcg tttagtatgg gcgtgcacag ggttggaggt
     6421 aggcaggggt caacctttag gcgttggtgt tagtgggcat ccattgctaa acaaatatga
     6481 tgatgtagaa aatagtggtg ggtatggtgg taatcctggt caggataata gggttaatgt
     6541 aggtatggat tataaacaaa cccagctatg tatggtgggc tgtgctccac cgttaggtga
     6601 acattggggt aagggtacac aatgttcaaa tacctctgta caaaatggtg actgcccccc
     6661 gttggaactt attaccagtg ttatacagga tggggacatg gttgatacag gctttggtgc
     6721 tatgaatttt gcagacttac aaaccaataa atcggatgtt ccccttgata tttgtggaac
     6781 tgtctgcaaa tatcctgatt atttgcaaat ggctgcagac ccttatggtg ataggttgtt
     6841 tttttatttg cgaaaggaac aaatgtttgc tagacacttt tttaataggg ccggtactgt
     6901 gggggaacct gtgcctgatg acctgttggt aaaagggggt aataacagat catctgtagc
     6961 tagtagtatt tatgtacata cacctagtgg ctcattggtg tcttcagagg ctcaattatt
     7021 taataaacca tattggcttc aaaaggctca gggacataac aatggtattt gctggggaaa
     7081 ccacttgttt gttactgtgg tagataccac acgcagtaca aatatgacac tatgtgcatc
     7141 tgtgtctaaa tctgctacat acactaattc agattataag gaatacatgc gccatgtgga
     7201 ggagtttgat ttacagttta tttttcaatt gtgtagcatt acattatctg cagaagtcat
     7261 ggcctatata cacacaatga atccttctgt tttggaggac tggaactttg gtttatcgcc
     7321 tccaccaaat ggtacactgg aggatactta tagatatgta cagtcacagg ccattacctg
     7381 tcagaaaccc acacctgaaa aagaaaaaca ggatccctat aaggatatga gtttttggga
     7441 ggttaactta aaagaaaagt tttcaagtga attagatcag tttccccttg gacgtaagtt
     7501 tttattgcaa agtggatatc gaggacggac gtctgctcgt acaggtataa agcgcccagc
     7561 tgtgtctaag ccctctacag cccccaaacg aaaacgtacc aaaaccaaaa agtaatatat
     7621 gtgtgtcaga tcgatgtcga cgtagttaac tagcgtacga tcgactgcca ggcatcaaat
     7681 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtccggcca
     7741 tcatggccgc ggtgatca
//
