LOCUS       40924_9096        7432 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCB112-Edim6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Dennehy M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2005) Virology, University of Cape Town, Anzio 
            Road Observatory, Cape Town, WP 7925, South Africa
REFERENCE   3  (bases 1 to 7432)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7432)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2005) Virology, University of Cape Town, Anzio Road 
            Observatory, Cape Town, WP 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      163..168
                     /label=derived from hsp60 promoter
                     /note="derived from hsp60 promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      186..191
                     /label=derived from hsp60 promoter
                     /note="derived from hsp60 promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    198
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      364..370
                     /regulatory_class="ribosome_binding_site"
     CDS             382..1749
                     /codon_start=1
                     /product="BCG hsp60-lysA chimera"
                     /label=BCG hsp60-lysA chimera
                     /protein_id="AAY46598.1"
                     /translation="MAKTIADPDVNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQ
                     EYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTG
                     GELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGI
                     VQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSH
                     IGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPI
                     AELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRR
                     YVSVDGGMSDNIRTGLYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPTI
                     FGPAIWLRLPPPALLLFAVESLQHGRPSRCGSGARGNARLVLRRETVDDLLSLEVR"
     CDS             1877..2689
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3771..5666
                     /note="OriM; mycobacterial origin of replication region;
                     similar to pAL5000 in GenBank Accession Number M60875"
     regulatory      5873..5878
                     /label=derived from Mycobacterium leprae 18 kDa promoter
                     /note="derived from Mycobacterium leprae 18 kDa promoter"
                     /regulatory_class="minus_35_signal"
     regulatory      5896..5901
                     /label=derived from Mycobacterium leprae 18 kDa promoter
                     /note="derived from Mycobacterium leprae 18 kDa promoter"
                     /regulatory_class="minus_10_signal"
     misc_feature    5910
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      5960..5966
                     /regulatory_class="ribosome_binding_site"
     CDS             5975..7303
                     /codon_start=1
                     /product="alpha antigen-rotavirus EDIM VP6 chimera"
                     /label=alpha antigen-rotavirus EDIM VP6 chimera
                     /protein_id="AAY46600.1"
                     /translation="MATDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFGGP
                     MDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNW
                     NFDFGLLGTTLLNLDANYVESARTTIDYFVDFIDNVCMDEMMRESQRNGIAPQSDALRK
                     LSGVKFRRINFNNSSEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLM
                     GTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPR
                     VINSADGATTWYFNPVILRPNNVEVEFLLNGQVINTYQARFGTIVARNFDTIRLSFQLM
                     RPPNMTPAVTALFPNAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYA
                     IPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVASIRSMLVK"
     terminator      7350..7396
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccaag acaattgcgg atccagatgt gaacgagctg
      421 ctgcacttag cgccgaatgt gtggccgcgc aatactactc gcgatgaagt cggtgtggtc
      481 tgcatcgcag gaattccact gacgcagctc gcccaggagt acgggacccc gctgttcgtc
      541 atcgacgagg acgactttcg ctcgcgctgc cgagaaaccg ccgcggcctt tggaagtggg
      601 gcgaacgtgc actatgccgc caaggcgttc ctgtgcagcg aagtagcccg gtggatcagc
      661 gaagaagggc tctgtctgga cgtttgcacc ggtggggagt tggcggtcgc gctgcacgct
      721 agctttccgc ccgagcgaat taccttgcac ggcaacaaca aatcggtctc agagttgacc
      781 gctgcggtca aagccggagt cggccatatt gtcgtcgatt cgatgaccga gatcgagcgc
      841 ctcgacgcca tcgcgggcga ggccggaatc gtccaggatg tcctggtgcg tctcaccgtc
      901 ggtgtcgagg cgcacaccca cgagttcatc tccaccgcgc acgaggacca gaaattcggg
      961 ttatcggtgg ccagcggcgc ggccatggca gcggtgcggc gcgttttcgc cactgatcac
     1021 ctgcgcctgg ttgggctaca cagccacatc ggttcgcaga tcttcgacgt ggacggcttc
     1081 gaactcgccg cgcaccgtgt catcggcctg ctacgcgacg tcgtcggcga gttcggtccc
     1141 gaaaagacgg cacagatcgc gaccgtcgat ctcggtggcg gcttgggcat ctcgtatttg
     1201 ccgtccgacg acccaccgcc gatagccgag ctcgcggcca agctgggtac catcgtgagc
     1261 gacgagtcaa cggccgtggg gctgccgacg cccaagctcg ttgtggagcc cggacgcgcc
     1321 atcgccggac cgggcaccat cacgttgtat gaggtcggca ccgttaagga cgtcgatgtc
     1381 agcgccacag cgcatcgacg ttacgtcagt gtcgacggcg gcatgagcga caacatccgc
     1441 accggcctct acggcgcgca gtatgacgtc cggctggtgt ctcgagtcag cgacgccccg
     1501 ccggtaccgg cccgtctggt cggaaagcac tgcgaaagtg gcgatatcat cgtgcgggac
     1561 acctgggtgc cgacgatatt cggcccggcg atctggttgc ggttgccgcc accggcctta
     1621 ctgctattcg ctgtcgagtc gttacaacat ggtcggccgt cccgctgtgg tagcggtgca
     1681 cgcggcaacg ctcgcctggt cctgcgtcgg gagacggtcg acgatttgct gagtttggaa
     1741 gtgaggtgaa ctagtctcta gccaacaaag cgacgttgtg tctcaaaatc tctgatgtta
     1801 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1861 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1981 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa
     2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2161 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2401 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac
     2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2521 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2581 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2641 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2701 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     2761 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     2821 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     2881 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     2941 gagtcagcaa caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac
     3781 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc
     3841 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc
     3901 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc
     3961 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg
     4021 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt
     4081 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac
     4141 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc
     4201 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac
     4261 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc
     4321 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc
     4381 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc
     4441 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac
     4501 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac
     4561 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac
     4621 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg
     4681 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc
     4741 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt
     4801 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc
     4861 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg
     4921 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg
     4981 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg
     5041 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg
     5101 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg
     5161 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg
     5221 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
     5281 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
     5341 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
     5401 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
     5461 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa
     5521 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
     5581 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
     5641 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagaata
     5701 gcccgcagcg ttcatagagc cgcagcgacg gcaccgggaa cgccggaaag ttcggccgct
     5761 accgatgatg tcgtatacgc tgcgttgcag tgccgacgta cccgtgccgg cactacaatc
     5821 ggtcatgagc aatctcctca gctgttcaga cagaaaactt gtctatcaca acttgcatca
     5881 atatatcgac cagtgctata tcaaatctat gtagtcagga acagctatat agttatagtt
     5941 tgtcacaaca gattggagtg cgaggtgacc acatatggcc acagacgtga gccgaaagat
     6001 tcgagcttgg ggacgccgat tgatgatcgg cacggcagcg gctgtagtcc ttccgggcct
     6061 ggtggggctt gccggcggag cggcaaccgc gggcgcgttc ggtgggccca tggatgtgct
     6121 gtactctatc tcacgtacac tgaaagatgc tagggacaaa attgttgagg gtacgctgta
     6181 ctctaacgtt agtgatctta ttcaacaatt taatcaaatg ctggtcacaa tgaatggaaa
     6241 tgaatttcaa acggggggaa ttggtaactt accacttcgt aattggaatt tcgattttgg
     6301 tttattgggt actacattat taaacctgga cgcaaactat gttgaatcag ccaggaccac
     6361 aattgactac ttcgttgact tcatagataa tgtgtgcatg gatgaaatga tgcgagagtc
     6421 acaaagaaat ggaatagcgc cacaatcaga tgctctgcgg aagctgtcag gagttaagtt
     6481 cagacgaata aactttaaca actcgtcaga gtatatcgag aactggaacc ttcagaaccg
     6541 caggcaaagg acaggattca catttcacaa accgaacata tttccatact cagcatcatt
     6601 cacactaaat agatcgcagc cgcagcatga caacttaatg ggtacaatgt ggttgaatgc
     6661 tgggtcagaa atacaggttg caggattcga ctactcgtgc gcaattaatg ctccagctaa
     6721 cattcaacaa ttcgaacaca tagtgcaatt gcggagggta ttgactactg caaccattac
     6781 gctacttcca gatgcagaga ggtttagctt tccaagagtt attaactcag cagacggagc
     6841 aactacatgg tacttcaacc cagtgatatt gcgcccaaat aatgtagaag ttgagttcct
     6901 attgaacggt caggtcatca atacttacca ggccagattc ggcaccatcg ttgcaagaaa
     6961 ttttgacacc atacgccttt catttcaatt gatgagaccg ccaaacatga caccagcagt
     7021 gactgcactt ttcccaaatg ctcagccgtt cgaacaccat gcaacagtcg gactgacact
     7081 tagaattgac tcagcaattt gtgaatcagt tctcgcggat gcaagtgaaa ctatgttagc
     7141 caatgtgacg tcagtcagac aagaatacgc gataccagta ggaccagtgt ttccaccagg
     7201 aatgaattgg accgacctca ttaccaacta ctcaccatca agagaagaca acctacaacg
     7261 cgtatttaca gtagcttcca ttagaagcat gctggtaaag tgaatcgatg tcgacgtagt
     7321 taactagcgt acgatcgact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     7381 gggcctttcg ttttatctgt tgtttgtccg gccatcatgg ccgcggtgat ca
//
