LOCUS       62056_4845        2717 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pCAo-2, complete sequence.
ACCESSION   MN436805
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    44..50
                     /label=SapI
                     /note="SapI"
     misc_feature    52..54
                     /label=Beta
                     /note="Beta"
     misc_feature    56..59
                     /label=A
                     /note="A"
     misc_feature    61..66
                     /label=BsaI
                     /note="BsaI"
     protein_bind    131..152
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        167..197
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(258..314)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(666..671)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    673..676
                     /label=F
                     /note="F"
     misc_feature    678..680
                     /label=Gamma
                     /note="Gamma"
     misc_feature    complement(682..688)
                     /label=SapI
                     /note="SapI"
     misc_feature    692..731
                     /label=UNSX
                     /note="UNSX"
     rep_origin      complement(822..1410)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1501..2313)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(2314..2503)
                     /label=CAJAPHA3 plasmid promoter
                     /note="CAJAPHA3 plasmid promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgagctcttc agcaaggagt
       61 gagaccattg acagcgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      301 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gacactagtg
      661 agcacggtct cacgctatac tgaagagcac cccaggatac atagattacc acaactccga
      721 gcccttccac ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      841 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      901 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      961 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1021 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1081 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1141 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1201 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1261 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1321 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1381 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1441 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta
     1501 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     1561 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     1621 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     1681 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     1741 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     1801 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     1861 ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     1921 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     1981 ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc
     2041 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     2101 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     2161 ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt
     2221 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     2281 tgagcaagac gtttcccgtt gaatatggct cataacagaa cttattattt ccttcctctt
     2341 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     2401 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     2461 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
     2521 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     2581 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     2641 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     2701 tgattttgtg ccgagct
//
