LOCUS       62056_4405        2686 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pCAe-1, complete sequence.
ACCESSION   MN436800
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(44..49)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    51..54
                     /label=A
                     /note="A"
     misc_feature    56..58
                     /label=Alpha
                     /note="Alpha"
     misc_feature    complement(60..66)
                     /label=SapI
                     /note="SapI"
     protein_bind    130..151
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        166..196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(257..313)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(314..330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    665..671
                     /label=SapI
                     /note="SapI"
     misc_feature    673..675
                     /label=Omega
                     /note="Omega"
     misc_feature    677..680
                     /label=B
                     /note="B"
     misc_feature    682..687
                     /label=BsaI
                     /note="BsaI"
     misc_feature    691..730
                     /label=UNSX
                     /note="UNSX"
     rep_origin      complement(821..1409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1503..2291)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      complement(2292..2439)
                     /label=specR promoter
                     /note="specR promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgaggtctca ggagaatgtg
       61 aagagcttga cagcgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      241 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
      301 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      481 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      541 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      601 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acactagtga
      661 gcacgctctt caggtatact tgagaccacc ccaggataca tagattacca caactccgag
      721 cccttccacc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1261 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtgattat
     1501 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     1561 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     1621 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     1681 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     1741 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     1801 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     1861 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     1921 ggctcgaaga taccagcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     1981 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     2041 cggagaatct cactctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     2101 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     2161 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     2221 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     2281 gcttccctca tgcgaaacga tcctcatcct gtctcttgat cagatattga tcccctgcgc
     2341 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     2401 ccagagggcg ccccagctgg caattccggt tcgcttgcta agcttgcatg cctgcaagtc
     2461 gactctagag gagcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     2521 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     2581 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     2641 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagct
//
