LOCUS       V000850                 6816 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000850
VERSION     V000850
KEYWORDS    pCAX APP Swe/Ind
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
   PUBMED   18160654
REFERENCE   2  (bases 1 to 6816)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2007 Dec 26. 27(52):14459-69."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..636
                     /label="chicken beta-actin promoter"
                     /note="contains a 21-bp deletion relative to the standard
                     chicken beta-actin promoter"
     intron          637..1653
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     primer_bind     1661..1680
                     /label="pCAG-F"
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             complement(2053..2064)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing
                     et al., 2004)"
                     /label="TEE"
                     /translation="NHKV"
     CDS             2544..2564
                     /label="7xHis"
                     /note="6xHis affinity tag"
     CDS             3514..3633
                     /gene="APP"
                     /label="Amyloid-beta precursor protein"
                     /note="Amyloid-beta precursor protein from Felis catus.
                     Accession#: P86906"
     primer_bind     complement(3837..3856)
                     /label="Bglob-pA-R"
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3902..3957
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3956..3975)
                     /label="rbglobpA-R"
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(4318..4334)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4342..4358)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4366..4396)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4411..4432)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4490..4686
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4692..4826
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4894..4911)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5065..5653)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5827..6684)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6685..6789)
                     /label="AmpR promoter"
     promoter        6816
                     /label="CAG"
                     /note="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg gggcggggcg gggcggggcg grgrggggcg
      541 gcggcrgcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      601 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      661 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      721 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      781 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      841 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      901 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      961 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1021 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg kgggggggtg agcagggggt
     1081 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1141 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1201 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1261 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1321 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1381 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1441 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1501 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1561 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1621 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1681 ctgtctcatc attttggcaa agaattcagg taccgatagc ttgccatgct gcccggtttg
     1741 gcactgctcc tgctggccgc ctggacggct cgggcgctgg aggtacccac tgatggtaat
     1801 gctggcctgc tggctgaacc ccagattgcc atgttctgtg gcagactgaa catgcacatg
     1861 aatgtccaga atgggaagtg ggattcagat ccatcaggga ccaaaacctg cattgatacc
     1921 aaggaaggca tcctgcagta ttgccaagaa gtctaccctg aactgcagat caccaatgtg
     1981 gtagaagcca accaaccagt gaccatccag aactggtgca agcggggccg caagcagtgc
     2041 aagacccatc cccactttgt gattccctac cgctgcttag ttggtgagtt tgtaagtgat
     2101 gcccttctcg ttcctgacaa gtgcaaattc ttacaccagg agaggatgga tgtttgcgaa
     2161 actcatcttc actggcacac cgtcgccaaa gagacatgca gtgagaagag taccaacttg
     2221 catgactacg gcatgttgct gccctgcgga attgacaagt tccgaggggt agagtttgtg
     2281 tgttgcccac tggctgaaga aagtgacaat gtggattctg ctgatgcgga ggaggatgac
     2341 tcggatgtct ggtggggcgg agcagacaca gactatgcag atgggagtga agacaaagta
     2401 gtagaagtag cagaggagga agaagtggct gaggtggaag aagaagaagc cgatgatgac
     2461 gaggacgatg aggatggtga tgaggtagag gaagaggctg aggaacccta cgaagaagcc
     2521 acagagagaa ccaccagcat tgccaccacc accaccacca ccacagagtc tgtggaagag
     2581 gtggttcgag ttcctacaac agcagccagt acccctgatg ccgttgacaa gtatctcgag
     2641 acacctgggg atgagaatga acatgcccat ttccagaaag ccaaagagag gcttgaggcc
     2701 aagcaccgag agagaatgtc ccaggtcatg agagaatggg aagaggcaga acgtcaagca
     2761 aagaacttgc ctaaagctga taagaaggca gttatccagc atttccagga gaaagtggaa
     2821 tctttggaac aggaagcagc caacgagaga cagcagctgg tggagacaca catggccaga
     2881 gtggaagcca tgctcaatga ccgccgccgc ctggccctgg agaactacat caccgctctg
     2941 caggctgttc ctcctcggcc tcgtcacgtg ttcaatatgc taaagaagta tgtccgcgca
     3001 gaacagaagg acagacagca caccctaaag catttcgagc atgtgcgcat ggtggatccc
     3061 aagaaagccg ctcagatccg gtcccaggtt atgacacacc tccgtgtgat ttatgagcgc
     3121 atgaatcagt ctctctccct gctctacaac gtgcctgcag tggccgagga gattcaggat
     3181 gaagttgatg agctgcttca gaaagagcaa aactattcag atgacgtctt ggccaacatg
     3241 attagtgaac caaggatcag ttacggaaac gatgctctca tgccatcttt gaccgaaacg
     3301 aaaaccaccg tggagctcct tcccgtgaat ggagagttca gcctggacga tctccagccg
     3361 tggcattctt ttggggctga ctctgtgcca gccaacacag aaaacgaagt tgagcctgtt
     3421 gatgcccgcc ctgctgccga ccgaggactg accactcgac caggttctgg gttgacaaat
     3481 atcaagacgg aggagatctc tgaagtgaat ctggatgcag aattccgaca tgactcagga
     3541 tatgaagttc atcatcaaaa attggtgttc tttgcagaag atgtgggttc aaacaaaggt
     3601 gcaatcattg gactcatggt gggcggtgtt gtcatagcga cagtgatctt catcaccttg
     3661 gtgatgctga agaagaaaca gtacacatcc attcatcatg gtgtggtgga ggttgacgcc
     3721 gctgtcaccc cagaggagcg ccacctgtcc aagatgcagc agaacggcta cgaaaatcca
     3781 acctacaagt tctttgagca gatgcagaac taggatatcg cggccgcctc gagagatctt
     3841 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     3901 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     3961 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     4021 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     4081 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     4141 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     4201 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     4261 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     4321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     4501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     4861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6781 ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
