LOCUS       40924_9076        7141 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-Mgen-recA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     RecA mediates MgpB and MgpC phase and antigenic variation in 
            Mycoplasma genitalium, but plays a minor role in DNA repair
  JOURNAL   Mol. Microbiol. 85 (4), 669-683 (2012)
  PUBMED    22686427
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2012"; volume: "85"; issue: "4"; pages: 
            "669-683"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2012) Synthetic Biology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3637..4737
                     /mol_type="other DNA"
                     /label=includes MG_338 5' end
                     /note="includes MG_338 5' end"
                     /db_xref="taxon:2097"
                     /organism="Mycoplasma genitalium"
     source          5761..6805
                     /mol_type="other DNA"
                     /label=includes MG_340 3' end
                     /note="includes MG_340 3' end"
                     /db_xref="taxon:2097"
                     /organism="Mycoplasma genitalium"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1822..2613)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4786..5442)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    6811..6871
                     /label=derived from Invitrogen TA Cloning Vector pCR-2.1
                     /note="derived from Invitrogen TA Cloning Vector pCR-2.1"
     promoter        complement(6885..6903)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6921..6937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6945..6961)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6969..6999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7014..7035)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     3601 gccgccagtg tgatggatat ctgcagaatt cgcccttagc agctgaactt tgtgatgaac
     3661 cactagaatt agcagtttgt aattgtttat caaactcttc aaataatttt gcagagtttg
     3721 tttgaccacc actttcacta ggatcttgaa aagcaggaaa gttataagaa agatttagat
     3781 tggaagctcc aattgcattt tcatcataaa tactgccaag tccagcaatt ggagattggt
     3841 aattgaaaga agcttgattt aataaaaatg ggttttcttc tattaatcac tgtgaaaata
     3901 aataagcttg aagattagta aaaaaatcac catttacatc atttaaattg ctgttaataa
     3961 acttaagttt attttctgtt ttttcaatgt tttctttagt tgttgcaacg cttgtatcac
     4021 ttgaattcac taaactaaca tttgcactaa tgttattgaa tattaaagat tgaaaatcag
     4081 taattaatct atcatagagt ttaagttttt ttcaactttc ttgattacca ccgaactgat
     4141 catattcttt ttgtacttca ataggtcact ttactctatc agcacgtttc ttttcatcta
     4201 tcaagttatc atgttcatct tcaacatttt cttcaaatat ctttaatcga ttagagatgt
     4261 ttctatctgc atttgcttgg tatcattttt gtaatgctga tccaaaacta acacgtaaga
     4321 tagaatcaac accactacca tcatttaaag cagactttaa aactacatct gggtttatga
     4381 tattttgatc actaaaacca attagttttt gggatcgatt gagagtagga aaaagattag
     4441 cagtgtttat tgtggaacag gctgtaatta ttgctgttgc acccactcca ctaaatgcta
     4501 aagcaattaa tgatcagcgc ttaaaacgat gagccatctt atactagcag tttgataatt
     4561 atcacattta aaaatctagc taatataatt gcaaatctag attattaaaa aatgtatatt
     4621 aattataaat aagttaattt ttctaattta agcattaaaa aagcttaaat ctctctaatt
     4681 aaaatgcttt accagaaaat caaaaaatta gtttttagct agctgtttgt tgaaatgttt
     4741 aatttagatc ttttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     4801 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     4861 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     4921 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     4981 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     5041 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     5101 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     5161 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     5221 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     5281 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     5341 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     5401 ggatatatca acggtggtat atccagtgat ttttttctcc atttaagatc ttatatttcc
     5461 tttctctatt aagtagtgtt tttattaaaa gcaaatgtaa ttaaaattac attgaatata
     5521 taagagaaaa ataacattaa taataattat taattgcttc taaccctact ttatacaatt
     5581 tatatctttc tttttttctt tcccttatag tagcaattgt acataatttt ttaaaaaaaa
     5641 caataaatat ttcttaaaaa aacaaaaaaa gattttccaa ataaattgct taatcttttt
     5701 ttgttttttc gtcttctttt ggttcagaaa ctggtgcttc actgttttct tgttcactaa
     5761 cttttaattc taatactctt cagcaactgt tttgctacct aagaaagtta actcttcatc
     5821 agttaaaaat cctgttcctg ctggaatttt tccaccaata ataacattct cttttaaacc
     5881 taaaagatag tctacttggt tcttaacagc agcatcagtt aggatttttt tggtatcttg
     5941 gaaggatgca gcacttaaaa aggagttaga tttacttgcc gcttcatcaa ttccaaacac
     6001 ctgattaatt gcaataacag gcatctttcc agctaaaagt aagctcttat ttagttcatt
     6061 gagataatgg ctatgcacta actgaccaac aaataaattg ctgttacctg catctgttac
     6121 ttgcaacaaa ttagttagtt gtctgataat aatttctaca tacttatcag caatatctat
     6181 cccttgaatc ctatacactt tttggatctc tacaatcatg tattgtctta ccctttggat
     6241 acctgcaatt cgcaaaagtt gtttaatatc aacagaacct tctgtaatct tagaacctgg
     6301 tgaaacttga tcaccaacat gaacacgtat ttgagcactg aaagggatag tataaatcct
     6361 ctcatcaacg tttgatttaa tcactacttc ctgagcgttt tgaacagtag taattgattt
     6421 aactgttcct ttcacttcag agataactgc tttttcaaaa tctttaggag taactacttc
     6481 aaagatctgt tttaaacgtt caaagccttg tgctaagttg ttttcagttg aaactccccc
     6541 agtatggaaa gtacgcattg tcaattgggt cccaggttca ccaattgatt gagcagcaat
     6601 caccccaaca gcagttccca attccaccaa cttaccagtt gacaagtcaa tgccaaagca
     6661 gtattgacag ataccatttt ccctttcaca atagataaca gatctaacta aaacttcttt
     6721 aatagatgtt gcacaaatct gtttagctaa ttgcgttgta ataagactgt tagcttctac
     6781 aatagttttt tgtgtttcag gatcaaaggg cgaattccag cacactggcg gccgttacta
     6841 gtggatccga gctcggtacc aagcttgatg catagcttga gtattctata gtgtcaccta
     6901 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7141 t
//
