LOCUS       40924_9071        4454 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-3', complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Sarafanov AG, Kondratov RV, Zakhar'ev VM, Prasolov VS, Kozlov,Iu.V.
  TITLE     [Gene for the human tissue activator plasminogen: regulation of 
            expression by signals, located in the 3'-untranslated region]
  JOURNAL   Mol. Biol. (Mosk.) 33 (3), 413-422 (1999)
  PUBMED    10519114
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2002) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   3  (bases 1 to 4454)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4454)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            (Mosk.)"; date: "1999"; volume: "33"; issue: "3"; pages: "413-422"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2002) Biochemistry, American Red Cross, Holland Laboratory, 
            15601 Crabbs Branch Way, Rockville, MD 20855, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     363..379
                     /label=pUC/M13 forward primer
                     /note="pUC/M13 forward primer"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        412..608
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             646..1302
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     intron          1471..1536
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1666..1686
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     primer_bind     1724..1740
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1768..1784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1811..1945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1947..2183
                     /label=SV40 enhancer
                     /note="SV40 enhancer"
                     /regulatory_class="enhancer"
     primer_bind     complement(2235..2251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2259..2275)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2283..2313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2328..2349)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2637..3223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3397..4254)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4255..4359)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccagatcgat ctgcatctca
      421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
      541 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
      601 tttgcaaaaa gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      661 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      721 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      781 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      841 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      901 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      961 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1021 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     1081 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1141 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1201 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     1261 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1321 tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
     1381 gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     1441 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     1501 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     1561 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     1621 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     1681 aaggtagaag accccaagcg agctccaccg cggtggcggc cgctctagaa ctagtggatc
     1741 ccccgggacg cgtgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
     1801 cgtgatcagc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1921 caatgtatct tatcatgtct ggatctgaac catggagcgg agaatgggcg gaactgggcg
     1981 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     2041 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     2101 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     2161 accctaactg acacacattc cacagcagat cctctagagt cgacctgcag gcatgcaagc
     2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2821 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3001 aggattagca gagcgaggta tgtaggcggt gctaagagtt cttgaagtgg tggcctaact
     3061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3121 gaaaaagagt tggtagctct tgatccggca aacaaccacc gctggtagcg gtggtttttt
     3181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4381 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4441 gaggcccttt cgtc
//
