LOCUS       Exported                4653 bp DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(2754..4653,1..2753)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    208..229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        244..274
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    282..298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     306..322
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             369..419
                     /codon_start=1
                     /label=signal peptide
                     /translation="MKKLLFAIPLVVPFYAA"
     CDS             596..634
                     /codon_start=1
                     /label=E tag
                     /translation="GAPVPYPDPLEPR"
     CDS             644..1873
                     /codon_start=1
                     /label=pIII phage surface protein
                     /translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
                     DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
                     YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
                     SGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADEN
                     ALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPL
                     MNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFST
                     FANILRNKES"
     primer_bind     complement(1883..1899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2112..2567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2849..2953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2954..3814
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3985..4573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
       61 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      121 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      181 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      241 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      301 tcacacagga aacagctatg accatgatta cgccaagctt tggagccttt tttttggaga
      361 ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc tatgcggccc
      421 agccggccat gaccaggtga agctgcagca gtcaggacct ggcctggtgg cgccctcaca
      481 gagcctgtcc atcacatgca ccgtctcagg gttttcatta accagctatg gtgtacactg
      541 ggttcgccag cctccaggaa agggtctgga gtggctggga gtaatagcgg ccgcaggtgc
      601 gccggtgccg tatccggatc cgctggaacc gcgtgccgca tagactgttg aaagttgttt
      661 agcaaaacct catacagaaa attcatttac taacgtctgg aaagacgaca aaactttaga
      721 tcgttacgct aactatgagg gctgtctgtg gaatgctaca ggcgttgtgg tttgtactgg
      781 tgacgaaact cagtgttacg gtacatgggt tcctattggg cttgctatcc ctgaaaatga
      841 gggtggtggc tctgagggtg gcggttctga gggtggcggt tctgagggtg gcggtactaa
      901 acctcctgag tacggtgata cacctattcc gggctatact tatatcaacc ctctcgacgg
      961 cacttatccg cctggtactg agcaaaaccc cgctaatcct aatccttctc ttgaggagtc
     1021 tcagcctctt aatactttca tgtttcagaa taataggttc cgaaataggc agggtgcatt
     1081 aactgtttat acgggcactg ttactcaagg cactgacccc gttaaaactt attaccagta
     1141 cactcctgta tcatcaaaag ccatgtatga cgcttactgg aacggtaaat tcagagactg
     1201 cgctttccat tctggcttta atgaggatcc attcgtttgt gaatatcaag gccaatcgtc
     1261 tgacctgcct caacctcctg tcaatgctgg cggcggctct ggtggtggtt ctggtggcgg
     1321 ctctgagggt ggcggctctg agggtggcgg ctctgagggt ggcggttctg agggtggcgg
     1381 ctctgagggt ggcggttccg gtggcggctc cggttccggt gattttgatt atgaaaaaat
     1441 ggcaaacgct aataaggggg ctatgaccga aaatgccgat gaaaacgcgc tacagtctga
     1501 cgctaaaggc aaacttgatt ctgtcgctac tgattacggt gctgctatcg atggtttcat
     1561 tggtgacgtt tccggccttg ctaatggtaa tggtgctact ggtgattttg ctggctctaa
     1621 ttcccaaatg gctcaagtcg gtgacggtga taattcacct ttaatgaata atttccgtca
     1681 atatttacct tctttgcctc agtcggttga atgtcgccct tatgtctttg gcgctggtaa
     1741 accatatgaa ttttctattg attgtgacaa aataaactta ttccgtggtg tctttgcgtt
     1801 tcttttatat gttgccacct ttatgtatgt attttcgacg tttgctaaca tactgcgtaa
     1861 taaggagtct taataagaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1921 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1981 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2041 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     2101 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2161 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     2221 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2281 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     2341 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2401 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     2461 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     2521 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcagtct
     2581 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2641 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2701 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     2761 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2821 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3061 tcagttgggt gctcgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcat
     4321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4621 ttttgctcac atgttctttc ctgcgttatc ccc
//
