LOCUS       40924_8951        6213 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAMFhGNTIf1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    149..204
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(565..581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(589..605)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(613..643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(657..678)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        736..932
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    938..1072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1310..1898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2072..2929)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2930..3034)
                     /label=AmpR promoter
     enhancer        3065..3444
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3446..3723
                     /label=chicken beta-actin promoter
     misc_feature    3817..4696
                     /label=intron
                     /note="intron"
     CDS             4707..4961
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     CDS             4962..6176
                     /codon_start=1
                     /note="unnamed protein product; hGNTI"
                     /protein_id="SJL88472.1"
                     /translation="ALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRV
                     PTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGH
                     EETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPA
                     AVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDF
                     FPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHG
                     QFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKEL
                     GEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPLTWEGY
                     DPSWN"
ORIGIN
        1 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
       61 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
      121 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
      181 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
      241 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
      301 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
      361 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
      421 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
      481 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
      541 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
      661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      721 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      841 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
      901 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
      961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     1081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1201 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
     1261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1741 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3061 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3121 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3181 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3241 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3301 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3361 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3421 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3481 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     3541 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
     3601 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     3661 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     3721 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
     3781 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     3841 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     3901 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     3961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     4021 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     4081 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     4141 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     4201 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     4261 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     4321 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
     4381 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     4441 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     4501 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     4561 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     4621 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4681 tgtgaccggc ggctctagag tcgagaatga gatttccttc aatttttact gcagttttat
     4741 tcgcagcatc ctccgcatta gctgctccag tcaacactac aacagaagat gaaacggcac
     4801 aaattccggc tgaagctgtc atcggttact cagatttaga aggggatttc gatgttgctg
     4861 ttttgccatt ttccaacagc acaaataacg ggttattgtt tataaatact actattgcca
     4921 gcattgctgc taaagaagaa ggggtatctt tggataaaag ggctctcgat ggcgaccccg
     4981 ccagcctcac ccgggaagtg attcgcctgg cccaagacgc cgaggtggag ctggagcggc
     5041 agcgtgggct gctgcagcag atcggggatg ccctgtcgag ccagcggggg agggtgccca
     5101 ccgcggcccc tcccgcccag ccgcgtgtgc ctgtgacccc cgcgccggcg gtgattccca
     5161 tcctggtcat cgcctgtgac cgcagcactg ttcggcgctg cctggacaag ctgctgcatt
     5221 atcggccctc ggctgagctc ttccccatca tcgttagcca ggactgcggg cacgaggaga
     5281 cggcccaggc catcgcctcc tacggcagcg cggtcacgca catccggcag cccgacctga
     5341 gcagcattgc ggtgccgccg gaccaccgca agttccaggg ctactacaag atcgcgcgcc
     5401 actaccgctg ggcgctgggc caggtcttcc ggcagtttcg cttccccgcg gccgtggtgg
     5461 tggaggatga cctggaggtg gccccggact tcttcgagta ctttcgggcc acctatccgc
     5521 tgctgaaggc cgacccctcc ctgtggtgcg tctcggcctg gaatgacaac ggcaaggagc
     5581 agatggtgga cgccagcagg cctgagctgc tctaccgcac cgactttttc cctggcctgg
     5641 gctggctgct gttggccgag ctctgggctg agctggagcc caagtggcca aaggccttct
     5701 gggacgactg gatgcggcgg ccggagcagc ggcaggggcg ggcctgcata cgccctgaga
     5761 tctcaagaac gatgaccttt ggccgcaagg gtgtgagcca cgggcagttc tttgaccagc
     5821 acctcaagtt tatcaagctg aaccagcagt ttgtgcactt cacccagctg gacctgtctt
     5881 acctgcagcg ggaggcctat gaccgagatt tcctcgcccg cgtctacggt gctccccagc
     5941 tgcaggtgga gaaagtgagg accaatgacc ggaaggagct gggggaggtg cgggtgcagt
     6001 atacgggcag ggacagcttc aaggctttcg ccaaggctct gggtgtcatg gatgacctta
     6061 agtcgggggt tccgagagct ggctaccggg gtattgtcac cttccagttc cggggccgcc
     6121 gtgtccacct ggcgccccca ctgacgtggg agggctatga tcctagctgg aattagcacc
     6181 tgtcgactgg agacctgcag gcatgcaagc tcg
//
