LOCUS       .                      10915 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        920..1300
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             1419..2135
                     /label="ECFP"
                     /note="enhanced CFP"
     terminator      2155..2407
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(2472..2502)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2517..2538)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2729..3074
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3147..4169
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4213..4387
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(4465..4489)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4914..5705
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5795..6383
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6569..6709)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7053..7247)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7316..8386)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8818..9444)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(10745..10769)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
       61 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      121 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc acggtatcga
      181 taagctcgcg gatccctgaa agcgacgttg gatgttaaca tctacaaatt gccttttctt
      241 atcgaccatg tacgtaagcg cttacgtttt tggtggaccc ttgaggaaac tggtagctgt
      301 tgtgggcctg tggtctcaag atggatcatt aatttccacc ttcacctacg atggggggca
      361 tcgcaccggt gagtaatatt gtacggctaa gagcgaattt ggcctgtagg atccctgaaa
      421 gcgacgttgg atgttaacat ctacaaattg ccttttctta tcgaccatgt acgtaagcgc
      481 ttacgttttt ggtggaccct tgaggaaact ggtagctgtt gtgggcctgt ggtctcaaga
      541 tggatcatta atttccacct tcacctacga tggggggcat cgcaccggtg agtaatattg
      601 tacggctaag agcgaatttg gcctgtagga tccctgaaag cgacgttgga tgttaacatc
      661 tacaaattgc cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt
      721 gaggaaactg gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt
      781 cacctacgat ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg
      841 cctgtaggat ccgcgagctg gtcaatccca ttgcttttga agcagctcaa cattgatctc
      901 tttctcgatc gagggagatt tttcaaatca gtgcgcaaga cgtgacgtaa gtatccgagt
      961 cagtttttat ttttctacta atttggtcgt ttatttcggc gtgtaggaca tggcaaccgg
     1021 gcctgaattt cgcgggtatt ctgtttctat tccaactttt tcttgatccg cagccattaa
     1081 cgacttttga atagatacgc tgacacgcca agcctcgcta gtcaaaagtg taccaaacaa
     1141 cgctttacag caagaacgga atgcgcgtga cgctcgcggt gacgccattt cgccttttca
     1201 gaaatggata aatagccttg cttcctatta tatcttccca aattaccaat acattacact
     1261 agcatctgaa tttcataacc aatctcgata caccaaatcg actctagaaa gcttctgcag
     1321 gggcccgggg tcgacattta aatactagtg gatccggtac cgccaccatg aagcggccag
     1381 cggcgacgaa gaaggcgggg caggcgaaga agaagaagat ggtgagcaag ggcgaggagc
     1441 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1501 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1561 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1621 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1681 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1741 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1801 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1861 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1921 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1981 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2041 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2101 ccgggatcac tctcggcatg gacgagctgt acaagtagga gctcgaattt ccccgatcgt
     2161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2401 ctagatcggg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2461 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2521 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2581 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc
     2641 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
     2701 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
     2761 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
     2821 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
     2881 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     2941 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     3001 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     3061 atttggagag gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct
     3121 ttcgcagatc cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg
     3181 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     3241 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     3301 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     3361 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct
     3421 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     3481 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     3541 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     3601 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     3661 cgtccgtcgc gcaggctctc gatgatctga tgctttgggc cgaggactgc cccgaagtcc
     3721 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     3781 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     3841 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     3901 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg
     3961 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     4021 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     4081 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     4141 gccccagcac tcgtccgagg gcaaagaaat agagtagatg ccgaccggga tctgtcgatc
     4201 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
     4261 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     4321 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
     4381 ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     4441 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     4501 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     4561 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     4621 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     4681 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa
     4741 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     4801 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     4861 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     4921 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     4981 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5041 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     5101 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     5161 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     5221 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     5281 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     5341 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     5401 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     5461 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     5521 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     5581 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     5641 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     5701 tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5761 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5821 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5881 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5941 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6001 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6061 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6121 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6181 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6241 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6301 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6361 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6421 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6481 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6541 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     6601 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6661 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     6721 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6781 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6841 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     6901 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     6961 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     7021 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     7081 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     7141 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     7201 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     7261 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     7321 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     7381 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     7441 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     7501 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     7561 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     7621 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     7681 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     7741 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     7801 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     7861 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     7921 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     7981 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     8041 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     8101 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     8161 gctcgtcgcc cttgggcggc ttcataatcg acggcgcacc ggctgccggc ggttgccggg
     8221 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     8281 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     8341 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
     8401 cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca
     8461 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
     8521 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
     8581 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
     8641 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
     8701 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
     8761 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
     8821 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
     8881 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
     8941 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
     9001 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
     9061 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
     9121 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
     9181 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
     9241 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
     9301 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
     9361 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
     9421 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
     9481 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
     9541 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
     9601 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
     9661 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
     9721 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
     9781 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
     9841 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
     9901 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
     9961 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    10021 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    10081 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    10141 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    10201 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    10261 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    10321 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    10381 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    10441 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    10501 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    10561 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    10621 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
    10681 cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc
    10741 ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa
    10801 cgacaatctg atccaagctc aagctgctct agcattcgcc attcaggctg cgcaactgtt
    10861 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa ggggg
//
