LOCUS       .                      10858 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        920..1300
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             1362..2078
                     /label="EYFP"
                     /note="enhanced YFP"
     terminator      2098..2350
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(2415..2445)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2460..2481)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2672..3017
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3090..4112
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4156..4330
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(4408..4432)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4857..5648
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5738..6326
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6512..6652)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6996..7190)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7259..8329)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8761..9387)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(10688..10712)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
       61 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      121 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc acggtatcga
      181 taagctcgcg gatccctgaa agcgacgttg gatgttaaca tctacaaatt gccttttctt
      241 atcgaccatg tacgtaagcg cttacgtttt tggtggaccc ttgaggaaac tggtagctgt
      301 tgtgggcctg tggtctcaag atggatcatt aatttccacc ttcacctacg atggggggca
      361 tcgcaccggt gagtaatatt gtacggctaa gagcgaattt ggcctgtagg atccctgaaa
      421 gcgacgttgg atgttaacat ctacaaattg ccttttctta tcgaccatgt acgtaagcgc
      481 ttacgttttt ggtggaccct tgaggaaact ggtagctgtt gtgggcctgt ggtctcaaga
      541 tggatcatta atttccacct tcacctacga tggggggcat cgcaccggtg agtaatattg
      601 tacggctaag agcgaatttg gcctgtagga tccctgaaag cgacgttgga tgttaacatc
      661 tacaaattgc cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt
      721 gaggaaactg gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt
      781 cacctacgat ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg
      841 cctgtaggat ccgcgagctg gtcaatccca ttgcttttga agcagctcaa cattgatctc
      901 tttctcgatc gagggagatt tttcaaatca gtgcgcaaga cgtgacgtaa gtatccgagt
      961 cagtttttat ttttctacta atttggtcgt ttatttcggc gtgtaggaca tggcaaccgg
     1021 gcctgaattt cgcgggtatt ctgtttctat tccaactttt tcttgatccg cagccattaa
     1081 cgacttttga atagatacgc tgacacgcca agcctcgcta gtcaaaagtg taccaaacaa
     1141 cgctttacag caagaacgga atgcgcgtga cgctcgcggt gacgccattt cgccttttca
     1201 gaaatggata aatagccttg cttcctatta tatcttccca aattaccaat acattacact
     1261 agcatctgaa tttcataacc aatctcgata caccaaatcg actctagaaa gcttctgcag
     1321 gggcccgggg tcgacattta aatactagtg gatccggtac catggtgagc aagggcgagg
     1381 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1441 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1501 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     1561 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1621 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1681 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1741 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1801 acagccagaa cgtctatctg atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1861 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1921 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     1981 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2041 ccgccgggat cactctcggc atggacgagc tgtacaagta ggagctcgaa tttccccgat
     2101 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2161 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2221 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2281 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2341 ttactagatc gggaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     2401 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2461 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2581 ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca
     2641 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
     2701 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     2761 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     2821 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     2881 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     2941 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     3001 ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
     3061 gctttcgcag atccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3121 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3181 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3241 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3301 cgctcccgat tccggaagtg cttgacattg gggagtttag cgagagcctg acctattgca
     3361 tctcccgccg ttcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3421 ttctacaacc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt agccagacga
     3481 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3541 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3601 gtgcgtccgt cgcgcaggct ctcgatgatc tgatgctttg ggccgaggac tgccccgaag
     3661 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3721 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3781 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3841 ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc
     3901 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     3961 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4021 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4081 gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg ggatctgtcg
     4141 atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
     4201 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     4261 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     4321 aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     4381 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     4441 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     4501 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     4561 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     4621 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc
     4681 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     4741 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     4801 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     4861 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     4921 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     4981 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     5041 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     5101 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     5161 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     5221 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     5281 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     5341 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     5401 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     5461 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     5521 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     5581 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     5641 aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact
     5701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5881 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6361 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6421 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6481 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     6541 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     6601 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     6661 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6721 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6781 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     6841 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     6901 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     6961 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     7021 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     7081 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     7141 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     7201 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     7261 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     7321 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     7381 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     7441 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     7501 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     7561 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     7621 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     7681 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     7741 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     7801 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     7861 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     7921 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     7981 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     8041 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     8101 gttgctcgtc gcccttgggc ggcttcataa tcgacggcgc accggctgcc ggcggttgcc
     8161 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     8221 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     8281 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
     8341 gggcttgggg gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg
     8401 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
     8461 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
     8521 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
     8581 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
     8641 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
     8701 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
     8761 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
     8821 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
     8881 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
     8941 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
     9001 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
     9061 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
     9121 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
     9181 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
     9241 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
     9301 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
     9361 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
     9421 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
     9481 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
     9541 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
     9601 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
     9661 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
     9721 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
     9781 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
     9841 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
     9901 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
     9961 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    10021 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    10081 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    10141 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    10201 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    10261 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    10321 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    10381 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    10441 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    10501 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    10561 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    10621 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
    10681 ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg
    10741 aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg ctgcgcaact
    10801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaaggggg
//
