LOCUS       .                      10899 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        920..1300
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             1362..2078
                     /label="EYFP"
                     /note="enhanced YFP"
     CDS             2094..2114
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     terminator      2139..2391
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(2456..2486)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2501..2522)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2713..3058
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3131..4153
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4197..4371
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(4449..4473)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4898..5689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5779..6367
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6553..6693)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7037..7231)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7300..8370)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8802..9428)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(10729..10753)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
       61 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      121 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc acggtatcga
      181 taagctcgcg gatccctgaa agcgacgttg gatgttaaca tctacaaatt gccttttctt
      241 atcgaccatg tacgtaagcg cttacgtttt tggtggaccc ttgaggaaac tggtagctgt
      301 tgtgggcctg tggtctcaag atggatcatt aatttccacc ttcacctacg atggggggca
      361 tcgcaccggt gagtaatatt gtacggctaa gagcgaattt ggcctgtagg atccctgaaa
      421 gcgacgttgg atgttaacat ctacaaattg ccttttctta tcgaccatgt acgtaagcgc
      481 ttacgttttt ggtggaccct tgaggaaact ggtagctgtt gtgggcctgt ggtctcaaga
      541 tggatcatta atttccacct tcacctacga tggggggcat cgcaccggtg agtaatattg
      601 tacggctaag agcgaatttg gcctgtagga tccctgaaag cgacgttgga tgttaacatc
      661 tacaaattgc cttttcttat cgaccatgta cgtaagcgct tacgtttttg gtggaccctt
      721 gaggaaactg gtagctgttg tgggcctgtg gtctcaagat ggatcattaa tttccacctt
      781 cacctacgat ggggggcatc gcaccggtga gtaatattgt acggctaaga gcgaatttgg
      841 cctgtaggat ccgcgagctg gtcaatccca ttgcttttga agcagctcaa cattgatctc
      901 tttctcgatc gagggagatt tttcaaatca gtgcgcaaga cgtgacgtaa gtatccgagt
      961 cagtttttat ttttctacta atttggtcgt ttatttcggc gtgtaggaca tggcaaccgg
     1021 gcctgaattt cgcgggtatt ctgtttctat tccaactttt tcttgatccg cagccattaa
     1081 cgacttttga atagatacgc tgacacgcca agcctcgcta gtcaaaagtg taccaaacaa
     1141 cgctttacag caagaacgga atgcgcgtga cgctcgcggt gacgccattt cgccttttca
     1201 gaaatggata aatagccttg cttcctatta tatcttccca aattaccaat acattacact
     1261 agcatctgaa tttcataacc aatctcgata caccaaatcg actctagaaa gcttctgcag
     1321 gggcccgggg tcgacattta aatactagtg gatccggtac catggtgagc aagggcgagg
     1381 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1441 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1501 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     1561 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1621 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1681 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1741 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1801 acagccagaa cgtctatctg atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1861 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1921 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     1981 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2041 ccgccgggat cactctcggc atggacgagc tgtacaaggt acccgggatc cagcctaaga
     2101 agaagagaaa ggttggagga tagagctcga atttccccga tcgttcaaac atttggcaat
     2161 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     2221 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     2281 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     2341 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattcg
     2401 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa
     2641 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     2701 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     2761 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     2821 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     2881 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     2941 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     3001 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg
     3061 ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca gatccggggg
     3121 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     3181 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     3241 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     3301 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     3361 gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc gttcacaggg
     3421 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga
     3481 ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     3541 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     3601 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     3661 tctcgatgat ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     3721 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     3781 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     3841 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     3901 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     3961 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     4021 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     4081 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     4141 gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag ctcgagtttc
     4201 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     4261 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     4321 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     4381 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     4441 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     4501 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     4561 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     4621 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     4681 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
     4741 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     4801 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     4861 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     4921 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     4981 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     5041 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     5101 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     5161 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     5221 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     5281 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     5341 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     5401 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     5461 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     5521 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     5581 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     5641 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     5701 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5881 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6421 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6481 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6541 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6601 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     6661 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     6721 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     6781 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     6841 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     6901 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     6961 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     7021 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     7081 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     7141 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     7201 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     7261 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     7321 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     7381 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     7441 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     7501 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     7561 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     7621 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     7681 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     7741 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     7801 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     7861 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     7921 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     7981 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     8041 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     8101 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     8161 cggcttcata atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     8221 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     8281 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     8341 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     8401 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     8461 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     8521 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     8581 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     8641 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     8701 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     8761 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     8821 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     8881 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     8941 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     9001 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     9061 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     9121 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     9181 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     9241 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     9301 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     9361 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     9421 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     9481 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     9541 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     9601 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
     9661 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
     9721 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
     9781 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
     9841 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
     9901 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
     9961 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    10021 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    10081 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    10141 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    10201 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    10261 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    10321 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    10381 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    10441 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    10501 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    10561 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    10621 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    10681 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    10741 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa
    10801 gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10861 gcgggcctct tcgctattac gccagctggc gaaaggggg
//
