LOCUS       40924_8936        9753 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA_ASX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Lukan T, Machens F, Coll A, Baebler S, Messerschmidt K, Gruden K.
  TITLE     Plant X-tender: An extension of the AssemblX system for the assembly
            and expression of multigene constructs in plants
  JOURNAL   PLoS ONE 13 (1), e0190526 (2018)
  PUBMED    29300787
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Lukan T, Machens F, Coll A, Baebler S, Messerschmidt K, Gruden K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Department of Biotechnology and Systems 
            Biology, National Institute of Biology, Vecna pot 111, Ljubljana 
            1000, Slovenia
REFERENCE   3  (bases 1 to 9753)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2018"; volume: "13"; issue: "1"; pages: "e0190526"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-NOV-2017) Department of Biotechnology and Systems Biology, 
            National Institute of Biology, Vecna pot 111, Ljubljana 1000, 
            Slovenia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             350..652
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    690..739
                     /label=homology region B0
                     /note="homology region B0"
     misc_feature    complement(740..757)
                     /label=I-SceI restriction site
                     /note="I-SceI restriction site"
     primer_bind     complement(762..778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    981..1005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2305..2931
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3363..4433
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4502..4696
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5040..5180
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5366..5954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6044..6835)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7260..7284
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7362..7536)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7579..8601)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8669..9346)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9537..9558
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9573..9603
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9611..9627
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9635..9651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9682..9699
                     /label=I-SceI restriction site
                     /note="I-SceI restriction site"
ORIGIN
        1 gctacgctgt ttataagctt ggcttactaa aagccagata acagtatgcg tatttgcgcg
       61 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
      121 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
      181 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
      241 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
      301 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
      361 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
      421 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
      481 taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
      541 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
      601 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
      661 aggctccctt atacacagcc agtaagcttt aaggtttcag tcacactacg gatactttta
      721 caacgggagc agttattcaa ttaccctgtt atccctacgg cactggccgt cgttttacaa
      781 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      841 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      901 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
      961 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     1021 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     1081 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     1141 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     1201 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     1261 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     1321 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     1381 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     1441 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     1501 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     1561 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     1621 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1681 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1741 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1801 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1861 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1921 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1981 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     2041 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     2101 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     2161 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     2221 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     2281 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     2341 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     2401 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     2461 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     2521 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     2581 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     2641 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2701 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2761 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2821 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2881 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2941 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     3001 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     3061 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     3121 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     3181 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     3241 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     3301 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     3361 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     3421 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     3481 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     3541 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     3601 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     3661 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     3721 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     3781 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     3841 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     3901 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     3961 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     4021 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     4081 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     4141 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     4201 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     4261 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     4321 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     4381 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     4441 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     4501 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     4561 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     4621 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     4681 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     4741 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     4801 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     4861 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     4921 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4981 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     5041 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     5101 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     5161 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5221 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5281 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5701 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5761 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5821 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5881 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5941 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6001 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
     6061 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     6121 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     6181 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     6241 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     6301 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
     6361 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     6421 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     6481 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     6541 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     6601 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     6661 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     6721 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
     6781 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
     6841 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
     6901 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
     6961 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
     7021 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
     7081 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
     7141 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     7201 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     7261 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     7321 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg
     7381 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
     7441 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc
     7501 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc
     7561 cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
     7621 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
     7681 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
     7741 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
     7801 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
     7861 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
     7921 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     7981 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     8041 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     8101 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     8161 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     8221 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
     8281 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     8341 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     8401 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     8461 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     8521 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     8581 ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag ctcgagagag
     8641 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
     8701 cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
     8761 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     8821 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     8881 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     8941 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     9001 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     9061 ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga
     9121 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     9181 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     9241 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     9301 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     9361 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     9421 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9541 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9661 gaattcgagc tcggtacccg gtagggataa cagggtaata tccctattgt cccattaaaa
     9721 taggaaagtc tggtaactca ggacactgta tct
//
