LOCUS       62056_4820       13812 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(202..226)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        1292..1637
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1673..1797
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1971..2073
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2074..2751
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3074..3376
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3420..3544)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3568..5373
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             5380..5406
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     terminator      5424..5676
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(5741..5771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5786..5807)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5998..6343
                     /label=CaMV35S(short)
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     CDS             6416..7438
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    7482..7656
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(7734..7758)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8183..8974
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9064..9652
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9838..9978)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10322..10516)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10585..11655)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIMKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12087..12713)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
       61 gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc
      121 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
      181 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
      241 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
      301 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      361 ggcgaaaggg ggagctgctc tagcattcgc cattcaggct gcgcaactgt tgggaagggc
      421 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
      481 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      541 ccacggtatc gataagctcg cggatccctg aaagcgacgt tggatgttaa catctacaaa
      601 ttgccttttc ttatcgacca tgtacgtaag cgcttacgtt tttggtggac ccttgaggaa
      661 actggtagct gttgtgggcc tgtggtctca agatggatca ttaatttcca ccttcaccta
      721 cgatgggggg catcgcaccg gtgagtaata ttgtacggct aagagcgaat ttggcctgta
      781 ggatccctga aagcgacgtt ggatgttaac atctacaaat tgccttttct tatcgaccat
      841 gtacgtaagc gcttacgttt ttggtggacc cttgaggaaa ctggtagctg ttgtgggcct
      901 gtggtctcaa gatggatcat taatttccac cttcacctac gatggggggc atcgcaccgg
      961 tgagtaatat tgtacggcta agagcgaatt tggcctgtag gatccctgaa agcgacgttg
     1021 gatgttaaca tctacaaatt gccttttctt atcgaccatg tacgtaagcg cttacgtttt
     1081 tggtggaccc ttgaggaaac tggtagctgt tgtgggcctg tggtctcaag atggatcatt
     1141 aatttccacc ttcacctacg atggggggca tcgcaccggt gagtaatatt gtacggctaa
     1201 gagcgaattt ggcctgtagg atccgcgagc tggtcaatcc cattgctttt gaagcagctc
     1261 aacattgatc tctttctcga tcgagggaga ttgagacttt tcaacaaagg gtaatatcgg
     1321 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     1381 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     1441 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1501 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1561 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1621 ttcatttgga gaggacaact ctagaaagct tctgcagggg cccggggtcg acacaagttt
     1681 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     1741 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
     1801 gccgcgggtg atgctgccaa cttagcggcc gctaagttgg cagcatcacc cgacgcactt
     1861 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     1921 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     1981 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     2041 tcatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2101 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2161 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2221 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2281 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2341 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2401 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2461 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2521 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2581 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2641 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2701 cagtactgcg atgagtggca ggcggggcgt aatctagagg atccggctta ctaaaagcca
     2761 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
     2821 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga
     2881 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
     2941 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
     3001 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
     3061 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     3121 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     3181 ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat
     3241 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     3301 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     3361 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     3421 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     3481 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     3541 tggggtacca tggtagatct gactagttta cgtcctgtag aaaccccaac ccgtgaaatc
     3601 aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag
     3661 cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac
     3721 gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa
     3781 gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact
     3841 cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg
     3901 ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt
     3961 tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa
     4021 aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc
     4081 agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat
     4141 gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc
     4201 agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg
     4261 actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg
     4321 tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg
     4381 tcagtggcag tgaagggcca acagttcctg attaaccaca aaccgttcta ctttactggc
     4441 tttggtcgtc atgaagatgc ggacttacgt ggcaaaggat tcgataacgt gctgatggtg
     4501 cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct
     4561 tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact
     4621 gctgctgtcg gctttcagct gtctttaggc attggtttcg aagcgggcaa caagccgaaa
     4681 gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt
     4741 aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac
     4801 gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg
     4861 cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac
     4921 accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat
     4981 gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg
     5041 caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg
     5101 ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg
     5161 tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc
     5221 gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact
     5281 cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac
     5341 ttcggtgaaa aaccgcagca gggaggcaaa caagctagct acccatacga tgttccagat
     5401 tacgcttgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     5461 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     5521 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     5581 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     5641 aattatcgcg cgcggtgtca tctatgttac tagatcggga attcgtaatc atgtcatagc
     5701 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5761 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5821 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5881 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
     5941 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     6001 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     6061 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     6121 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     6181 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     6241 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     6301 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     6361 tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat gagatatgaa
     6421 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     6481 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6541 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6601 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6661 gtttagcgag agcctgacct attgcatctc ccgccgttca cagggtgtca cgttgcaaga
     6721 cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta tggatgcgat
     6781 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6841 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6901 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6961 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     7021 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     7081 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     7141 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccacgact
     7201 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     7261 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     7321 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     7381 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaagaaata
     7441 gagtagatgc cgaccgggat ctgtcgatcg acaagctcga gtttctccat aataatgtgt
     7501 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     7561 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     7621 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
     7681 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     7741 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     7801 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     7861 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     7921 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     7981 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     8041 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     8101 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     8161 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     8221 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     8281 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     8341 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     8401 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     8461 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     8521 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     8581 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     8641 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     8701 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     8761 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     8821 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     8881 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     8941 aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat
     9001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9181 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9421 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9481 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9541 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9601 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9661 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9721 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9781 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     9841 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     9901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     9961 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    10021 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    10081 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
    10141 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
    10201 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
    10261 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
    10321 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
    10381 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
    10441 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
    10501 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
    10561 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
    10621 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
    10681 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
    10741 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
    10801 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
    10861 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
    10921 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
    10981 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
    11041 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
    11101 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
    11161 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    11221 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    11281 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    11341 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    11401 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcataatcga
    11461 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    11521 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    11581 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    11641 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    11701 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    11761 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    11821 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    11881 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    11941 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    12001 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    12061 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    12121 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    12181 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    12241 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    12301 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    12361 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    12421 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    12481 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    12541 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    12601 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    12661 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    12721 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    12781 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    12841 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    12901 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    12961 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    13021 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    13081 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    13141 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    13201 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    13261 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    13321 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    13381 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    13441 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    13501 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    13561 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    13621 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    13681 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    13741 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    13801 aacttaggac tt
//
