LOCUS       62056_4815       10549 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        238..583
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             609..1325
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="LTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1332..1349
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1381..1633
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1655..1679
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2979..3605
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4042..5106
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5175..5369
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5713..5853
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6039..6627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6717..7508)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7933..7957
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8035..8209)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8252..9274)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9342..10019)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10210..10231
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10246..10276
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10284..10300
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10308..10324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10334..10390
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta gcttcatgga
       61 gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg aacagttcat
      121 acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
      181 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      241 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      301 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      361 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      421 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      481 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      541 cccttcctct atataaggaa gttcatttca tttggagaga acacggggga ctcttgacca
      601 tggtagatct gactagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      661 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      721 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      781 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      841 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      901 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      961 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1021 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1081 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1141 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1201 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1261 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1321 acaaggctag ccaccaccac caccaccacg tgtgaattgg tgaccagctc gaatttcccc
     1381 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     1441 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     1501 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     1561 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     1621 atgttactag atcgggaatt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     1681 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
     1741 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     1801 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     1861 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     1921 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     1981 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     2041 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     2101 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     2161 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     2221 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     2281 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     2341 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     2401 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     2461 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     2521 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     2581 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     2641 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     2701 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     2761 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     2821 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     2881 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     2941 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     3001 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     3061 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     3121 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     3181 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     3241 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     3301 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     3361 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     3421 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     3481 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     3541 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     3601 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     3661 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     3721 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     3781 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     3841 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     3901 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     3961 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     4021 agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     4081 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     4141 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     4201 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     4261 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     4321 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     4381 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     4441 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     4501 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     4561 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     4621 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     4681 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     4741 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     4801 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     4861 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     4921 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     4981 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     5041 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     5101 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     5161 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     5221 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     5281 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     5341 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     5401 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     5461 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     5521 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     5581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5701 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     5761 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     5821 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     5881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
     6721 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     6781 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     6841 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     6901 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     6961 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     7021 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     7081 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     7141 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
     7201 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
     7261 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
     7321 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
     7381 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
     7441 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
     7501 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     7561 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     7621 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     7681 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     7741 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     7801 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     7861 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     7921 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
     7981 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg
     8041 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     8101 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     8161 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     8221 cgatcgacag atccggtcgg catctactct atttctttgc cctcggacga gtgctggggc
     8281 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     8341 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     8401 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     8461 caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc
     8521 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     8581 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     8641 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     8701 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     8761 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     8821 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     8881 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta
     8941 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     9001 agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga
     9061 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     9121 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     9181 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     9241 cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg ggatctgcga
     9301 aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
     9361 tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat
     9421 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     9481 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     9541 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     9601 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     9661 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     9721 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca
     9781 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     9841 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     9901 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     9961 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    10021 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    10081 gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg
    10141 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10201 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10261 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10321 tgaccatgat tacgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc
    10381 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    10441 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    10501 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatg
//
