LOCUS       62056_4810        9847 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        234..579
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             599..688
                     /codon_start=1
                     /label=3xMyc
                     /note="3 tandem Myc epitope tags"
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     misc_feature    733..789
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             803..868
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      894..1146
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1168..1192
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2492..3118
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3555..4619
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4688..4882
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5226..5366
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5552..6140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6230..7021)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7446..7470
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7548..7722)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7765..8787)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8855..9532)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9723..9744
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9759..9789
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9797..9813
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9821..9837
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttagctt catggagtca
       61 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
      121 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
      181 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
      241 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
      301 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
      361 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
      421 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      481 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      541 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct tgaccatgga
      601 acaaaaacta atatcagagg aagatttgga acagaaacta atcagtgaag aagatttaga
      661 gcaaaagtta atttccgagg aggacttaga gcagaagttg atttcagaag aagatctggg
      721 gcccataggc ctgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
      781 tgcaagctta ctagtcacgc gtgactacaa agaccatgac ggtgattata aagatcatga
      841 catcgattac aaggatgacg atgacaagta aggtgaccag ctcgaatttc cccgatcgtt
      901 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
      961 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     1021 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     1081 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     1141 tagatcggga attaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     1201 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
     1261 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     1321 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     1381 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     1441 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     1501 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     1561 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     1621 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     1681 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     1741 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     1801 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     1861 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     1921 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     1981 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     2041 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     2101 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     2161 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     2221 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     2281 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     2341 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     2401 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     2461 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     2521 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     2581 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     2641 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     2701 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     2761 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     2821 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     2881 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     2941 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     3001 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     3061 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     3121 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     3181 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     3241 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     3301 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     3361 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     3421 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     3481 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     3541 ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     3601 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     3661 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     3721 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     3781 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     3841 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     3901 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     3961 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     4021 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     4081 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     4141 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     4201 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     4261 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     4321 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     4381 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     4441 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     4501 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     4561 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     4621 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
     4681 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     4741 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     4801 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     4861 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     4921 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     4981 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     5041 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     5101 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5161 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5221 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     5281 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5341 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     5401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6181 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     6241 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     6301 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     6361 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     6421 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     6481 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     6541 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     6601 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     6661 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     6721 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     6781 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     6841 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     6901 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     6961 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     7021 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     7081 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     7141 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     7201 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     7261 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     7321 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     7381 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     7441 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     7501 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta
     7561 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
     7621 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta
     7681 attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga
     7741 cagatccggt cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt
     7801 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     7861 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     7921 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     7981 aatgcggagc atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc
     8041 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     8101 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     8161 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
     8221 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     8281 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     8341 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     8401 ctggctaaga tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc
     8461 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
     8521 ataggtcagg ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc
     8581 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     8641 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     8701 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     8761 cgtcgcggtg agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg
     8821 agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg
     8881 aacttcctta tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     8941 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     9001 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     9061 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     9121 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     9181 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     9241 attcttggag tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt
     9301 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     9361 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     9421 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     9481 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     9541 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     9601 catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9661 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9721 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9781 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9841 gattacg
//
