LOCUS       Exported               11319 bp DNA     circular SYN 03-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             184..1986
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="RPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAV
                     PGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVME
                     HQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFF
                     NYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVV
                     ATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLI
                     NHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWAD
                     EHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKN
                     HPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLF
                     DVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTD
                     MWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPK
                     SAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             1993..2010
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2045..2297
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2319..2343
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3644..4273
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4702..5775
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5841..6035
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6379..6519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7380..8174)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8599..8623
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8701..8875
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8882..9433)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9478..10154)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10345..10366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10381..10411
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10419..10435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10443..10459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10460..10687
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    10468..10524
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10528..10544)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10921..11266
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          join(11298..11319,1..168)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 tcattttctt ggttaggacc cttttctctt tttatttttt tgagctttga tctttcttta
       61 aactgatcta ttttttaatt gattggttat ggtgtaaata ttacatagct ttaactgata
      121 atctgattac tttatttcgt gtgtctatga tgatgatgat agttacagaa ccgacgactc
      181 gtccgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc
      241 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa
      301 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt
      361 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc
      421 cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat
      481 caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat
      541 gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag
      601 actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc
      661 catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac
      721 acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct
      781 gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa
      841 caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc
      901 tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag
      961 tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc
     1021 ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg
     1081 cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt
     1141 ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca
     1201 gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta
     1261 ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac
     1321 ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac
     1381 cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca
     1441 cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc
     1501 acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat
     1561 gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca
     1621 gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc
     1681 atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg
     1741 agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc
     1801 gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg
     1861 cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct
     1921 tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc
     1981 aaacaagcta gccaccacca ccaccaccac gtgtgaatta caggtgacca gctcgaattt
     2041 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     2101 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     2161 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     2221 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     2281 atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta
     2341 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     2401 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     2461 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     2521 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     2581 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     2641 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     2701 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     2761 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     2821 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     2881 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     2941 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     3001 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     3061 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     3121 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     3181 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     3241 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     3301 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     3361 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     3421 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     3481 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     3541 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     3601 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     3661 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     3721 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     3781 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     3841 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     3901 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     3961 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     4021 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     4081 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     4141 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     4201 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     4261 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     4321 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     4381 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     4441 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     4501 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     4561 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     4621 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     4681 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     4741 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     4801 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     4861 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     4921 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     4981 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     5041 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     5101 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     5161 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     5221 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     5281 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     5341 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     5401 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     5461 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     5521 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     5581 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     5641 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     5701 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     5761 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     5821 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     5881 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     5941 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     6001 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     6061 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     6121 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     6181 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     6241 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6301 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6361 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     6421 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
     7381 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     7441 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     7501 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     7561 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     7621 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     7681 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     7741 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     7801 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     7861 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     7921 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     7981 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     8041 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     8101 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     8161 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     8221 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     8281 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     8341 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     8401 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     8461 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     8521 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     8581 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     8641 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     8701 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     8761 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     8821 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     8881 gtcaaatctc ggtgacgggc aggaccggac ggggcggtac cggcaggctg aagtccagct
     8941 gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc
     9001 ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga
     9061 cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg gtgtagagcg
     9121 tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtcgac tcggccgtcc
     9181 agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc gatgccggcg acctcgccgt
     9241 ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg tccgtccact
     9301 cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg tagtggttga
     9361 cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc
     9421 gttctgggct catggtagac tcgagagaga tagatttgta gagagagact ggtgatttca
     9481 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     9541 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     9601 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     9661 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     9721 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     9781 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     9841 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     9901 gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     9961 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    10021 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    10081 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    10141 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    10201 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
    10261 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    10321 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10381 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10441 cacaggaaac agctatgaca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
    10501 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    10561 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    10621 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    10681 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
    10741 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
    10801 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
    10861 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
    10921 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
    10981 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
    11041 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
    11101 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
    11161 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
    11221 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
    11281 ccatggtaga tctgagggta aatttctagt ttttctcct
//
