LOCUS       Exported                8428 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            bialophos/phosphinothricin resistance and kanamycin resistance 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Cambia
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8428)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..76)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    279..303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1604..2230
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2662..3732
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3801..3995
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4339..4479
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4665..5253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5343..6134)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6559..6583
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6661..6835)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6845..7393)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7438..8114)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8305..8326
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8341..8371
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8379..8395
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8403..8419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8428
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
       61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
      241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
      301 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
      361 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      421 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
      481 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
      541 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
      601 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
      661 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
      721 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
      781 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
      841 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
      901 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
      961 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     1021 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     1081 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     1141 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     1201 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     1261 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     1321 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     1381 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     1441 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     1501 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     1561 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     1621 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     1681 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     1741 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     1801 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     1861 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     1921 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     1981 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     2041 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     2101 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     2161 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     2221 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     2281 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     2341 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     2401 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     2461 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     2521 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     2581 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     2641 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     2701 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     2761 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     2821 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     2881 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     2941 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     3001 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     3061 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     3121 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     3181 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     3241 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     3301 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     3361 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     3421 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     3481 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     3541 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     3601 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     3661 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     3721 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     3781 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     3841 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     3901 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     3961 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     4021 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     4081 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     4141 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     4201 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4261 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4321 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     4381 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     4441 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     4501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5101 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac
     5341 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     5401 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     5461 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     5521 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     5581 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     5641 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     5701 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     5761 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     5821 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     5881 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     5941 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     6001 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     6061 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     6121 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     6181 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     6241 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     6301 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     6361 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     6421 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     6481 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     6541 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     6601 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     6661 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     6721 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     6781 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     6841 gtcaaatctc ggtgacgggc aggaccggac ggggcggtac cggcaggctg aagtccagct
     6901 gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc
     6961 ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga
     7021 cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg gtgtagagcg
     7081 tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtcgac tcggccgtcc
     7141 agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc gatgccggcg acctcgccgt
     7201 ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg tccgtccact
     7261 cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg tagtggttga
     7321 cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc
     7381 gttctgggct catggtagac tcgagagaga tagatttgta gagagagact ggtgatttca
     7441 gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     7501 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     7561 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     7621 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     7681 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     7741 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     7801 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     7861 gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     7921 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     7981 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     8041 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     8101 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     8161 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
     8221 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8281 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8341 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8401 cacaggaaac agctatgaca tgattacg
//
