LOCUS       62056_4800        9764 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..350
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             396..1112
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      1134..1386
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1395..1411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1614..1638
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2938..3564
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3996..5066
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5135..5329
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5673..5813
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5999..6587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6677..7468)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7893..7917
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7995..8169)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8179..8727)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8772..9449)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9640..9661
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9676..9706
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9714..9730
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9738..9754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aatttgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
       61 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
      121 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
      181 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      241 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      301 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc
      361 tagaggatcc gaattcgagc tccgtcgaca agcttatggt gagcaagggc gaggagctgt
      421 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      481 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      541 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg
      601 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      661 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      721 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      781 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      841 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      901 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      961 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1021 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1081 ggatcactca cggcatggac gagctgtaca agtaaagcgg cccgaatttc cccgatcgtt
     1141 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     1201 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     1261 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     1321 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     1381 tagatcagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc
     1561 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     1621 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa
     1681 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     1741 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     1801 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     1861 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     1921 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     1981 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     2041 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     2101 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     2161 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     2221 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     2281 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     2341 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     2401 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     2461 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     2521 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     2581 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     2641 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     2701 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     2761 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     2821 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     2881 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     2941 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     3001 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     3061 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     3121 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     3181 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     3241 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     3301 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     3361 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     3421 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     3481 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     3541 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     3601 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     3661 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     3721 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     3781 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     3841 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     3901 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     3961 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
     4021 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
     4081 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
     4141 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
     4201 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
     4261 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
     4321 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
     4381 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
     4441 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
     4501 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
     4561 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
     4621 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
     4681 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
     4741 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
     4801 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
     4861 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
     4921 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
     4981 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
     5041 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
     5101 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
     5161 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
     5221 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
     5281 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
     5341 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
     5401 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
     5461 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
     5521 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5581 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5641 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     5701 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     5761 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     5821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6421 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6661 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     6721 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     6781 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     6841 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     6901 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     6961 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     7021 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     7081 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     7141 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     7201 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     7261 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     7321 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     7381 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     7441 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     7501 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     7561 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     7621 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
     7681 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     7741 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     7801 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     7861 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     7921 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
     7981 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     8041 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     8101 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     8161 atggagaaac tcgagtcaaa tctcggtgac gggcaggacc ggacggggcg gtaccggcag
     8221 gctgaagtcc agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc
     8281 ccgcagcatg ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt
     8341 gggcagcccg atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag
     8401 gtgggtgtag agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt
     8461 cgactcggcc gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc
     8521 ggcgacctcg ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag
     8581 gtcgtccgtc cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc
     8641 gatgtagtgg ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat
     8701 gtcggccggg cgtcgttctg ggctcatggt agactcgaga gagatagatt tgtagagaga
     8761 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt
     8821 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     8881 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     8941 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     9001 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     9061 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     9121 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     9181 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     9241 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     9301 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     9361 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     9421 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     9481 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
     9541 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9601 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9661 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9721 cggataacaa tttcacacag gaaacagcta tgaccatgat tacg
//
