LOCUS       .                       9733 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..350
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             396..1070
                     /label="DsRed2"
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     terminator      1103..1355
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1364..1380)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1583..1607
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2907..3533
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3965..5035
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5104..5298
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5642..5782
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5968..6556)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6646..7437)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7862..7886
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7964..8138)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8148..8696)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(8741..9418)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9609..9630
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9645..9675
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9683..9699
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     9707..9723
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aatttgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
       61 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
      121 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
      181 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      241 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      301 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc
      361 tagaggatcc gaattcgagc tccgtcgaca agcttatggc ctcctccgag aacgtcatca
      421 ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga
      481 tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga
      541 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
      601 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
      661 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc
      721 aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact
      781 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc
      841 gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg aagctgaagg
      901 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc
      961 tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca
     1021 ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagtgtacaa
     1081 gtaaagcggc ccgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     1141 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     1201 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     1261 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     1321 attatcgcgc gcggtgtcat ctatgttact agatcagctt ggcactggcc gtcgttttac
     1381 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1441 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1501 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
     1561 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     1621 gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     1681 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     1741 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     1801 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     1861 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     1921 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     1981 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     2041 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     2101 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     2161 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     2221 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     2281 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     2341 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     2401 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     2461 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     2521 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     2581 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     2641 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     2701 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     2761 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     2821 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     2881 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     2941 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     3001 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     3061 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     3121 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     3181 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     3241 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     3301 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     3361 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     3421 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     3481 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     3541 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     3601 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     3661 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     3721 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     3781 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     3841 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     3901 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     3961 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     4021 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     4081 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     4141 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     4201 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     4261 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     4321 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     4381 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     4441 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     4501 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     4561 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     4621 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     4681 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     4741 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     4801 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     4861 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     4921 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     4981 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     5041 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     5101 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     5161 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     5221 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     5281 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     5341 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     5401 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     5461 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     5521 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5581 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5641 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     5701 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5761 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5821 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5881 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5941 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6001 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6061 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6301 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6361 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6481 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6541 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6601 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     6661 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     6721 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     6781 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     6841 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     6901 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     6961 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     7021 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     7081 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     7141 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     7201 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     7261 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     7321 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     7381 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     7441 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
     7501 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
     7561 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
     7621 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
     7681 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
     7741 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
     7801 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
     7861 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
     7921 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
     7981 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     8041 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
     8101 ccttatctgg gaactactca cacattatta tggagaaact cgagtcaaat ctcggtgacg
     8161 ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca
     8221 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc
     8281 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg
     8341 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc
     8401 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt
     8461 gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc
     8521 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc
     8581 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc
     8641 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatggta
     8701 gactcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
     8761 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
     8821 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     8881 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     8941 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     9001 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     9061 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     9121 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
     9181 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     9241 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     9301 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     9361 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     9421 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     9481 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg
     9541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9601 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9661 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9721 gaccatgatt acg
//
