LOCUS       .                      10194 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1219..1845
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2277..3347
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3416..3610
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3954..4094
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4280..4868)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4958..5749)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6174..6198
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6276..6450)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6460..7242)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(7511..8189)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8380..8401
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8416..8446
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        8510..8855
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8870..9586
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     terminator      9608..9860
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10089..10113
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
     3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4921 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
     4981 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     5041 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     5101 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     5161 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     5221 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
     5281 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
     5341 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     5401 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
     5461 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     5521 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     5581 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
     5641 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
     5701 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
     5761 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
     5821 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
     5881 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
     5941 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
     6001 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
     6061 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
     6121 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     6181 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
     6241 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
     6301 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
     6361 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct
     6421 gggaactact cacacattat tatggagaaa ctcgagtcac ttgccaacga ccttagtaat
     6481 ctcgcccttg acgtaatgaa cgaactcctc cagctgatca gcccgggacg cgagcctgtc
     6541 ctcctcctgg cccaggtaag cctgcctagc ctcgaggatc acaggctggt actgagctgg
     6601 cagcctctcc atcgcccaat cagcagccac gtcctttgga gcaatcttcc ccgtgacagc
     6661 agagtaccag atgcgcgaca gggtgaggac cacattcctc tcatcgccag cccagtcggg
     6721 cggagaattc cacagcgtga gggtctcgtt cagggcctcg aagagatcct gctcagggac
     6781 tgggtcgaag agctcctcag cagcagggcc aaccagagcg acggagtgct ccctagcctt
     6841 agtcaggagg atagccagat caatgtcgat tgtcgctggc tcgaaaatcc cggccaggat
     6901 gtcattgcgc tgccactcgc cgaactggag ctccctctta gctgggtacc tccatggaat
     6961 gatatcgtca tgaacgacaa tcgtcacctc aaccgccctg aggatctcgg actcgcctgg
     7021 cgaagcgctg gtctccagga gatcgttaat gagagccctc ctcgtggtct cgtccagccg
     7081 gacagtcact gtaaccagga gatcgatgtc ggagtggggc ttcaggccac catcaaccgc
     7141 tgacccgtag agatggacag ccaggagggt tggctccagg tgcctctcga tcacgcccac
     7201 aacctcgctg agctgagtgg agacctcagc gatcacggcc tcagcctcag ctgtagcgac
     7261 ggaagctgag atgcggaagc tggacttgcg gaccacccgg agcaccgagt ccttcttgaa
     7321 aacccccagt gacttggcag agatctgtgt cagcttcttg ctgccgaaga agagagagtt
     7381 cgacacgagc ggccccttct gcgtcttgga gagattagag gagttgagcg acagcgtctg
     7441 aatgccctga gccatcgacc tgatctgagc cattcgagag agatagattt gtagagagag
     7501 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     7561 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     7621 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     7681 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     7741 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     7801 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     7861 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     7921 cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     7981 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     8041 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     8101 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     8161 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     8221 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
     8281 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8341 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8401 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8461 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattct gagacttttc
     8521 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
     8581 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
     8641 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     8701 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     8761 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     8821 ctatataagg aagttcattt catttggaga gaacacgggg gacgagctca tggtgagcaa
     8881 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     8941 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     9001 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     9061 cttcacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     9121 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     9181 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     9241 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     9301 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     9361 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     9421 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     9481 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     9541 cgtgaccgcc gccgggatca ctcacggcat ggacgagctg gacaagtagg tcgacctgca
     9601 ggcatgcgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     9661 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     9721 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     9781 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     9841 gtcatctatg ttactagatc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     9901 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9961 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    10021 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
    10081 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
    10141 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtg
//
