LOCUS       V014977                 6312 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014977
VERSION     V014977
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(979..1026)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1261..2052)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2087..2444)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2446..2550)
                     /label="AmpR promoter"
     polyA_signal    complement(2579..2700)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2826..3542)
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        complement(3610..3813)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4473..5490
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             5594..6100
                     /gene="Cdkn2a"
                     /label="Tumor suppressor ARF"
                     /note="Tumor suppressor ARF from Mus musculus. Accession#:
                     Q64364"
     polyA_signal    6170..6225
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
ORIGIN
        1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt
      841 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
      901 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
      961 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta
     1021 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc
     1081 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     1141 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     1201 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     1261 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     1321 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     1381 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     1441 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     1501 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag
     1561 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     1621 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     1681 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     1741 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     1801 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     1861 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     1921 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     1981 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     2041 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta
     2101 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     2161 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     2221 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     2281 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     2341 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     2401 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
     2461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgctta
     2581 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2641 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2701 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2761 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg
     2821 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     2881 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     2941 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     3001 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     3061 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     3121 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     3181 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     3241 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     3301 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     3361 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     3421 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     3481 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     3541 atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actgcaggga tctgacggtt
     3601 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     3661 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     3721 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     3781 acgcccattg atgtactgcc aaaaccgcat cacgacattg attattgact agttattaat
     3841 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3901 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3961 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     4021 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     4081 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     4141 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     4201 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     4261 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc
     4321 ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg
     4381 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     4441 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg
     4501 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     4561 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     4621 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     4681 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     4741 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     4801 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     4861 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     4921 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     4981 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     5041 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     5101 gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     5161 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     5221 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     5281 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     5341 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     5401 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     5461 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     5521 tctcatcatt ttggcaaaga attcctcgag gaattcactc ctcgctagca ggtgcaggct
     5581 gaagcttgcc accatgggtc gcaggttctt ggtcactgtg aggattcagc gcgcgggccg
     5641 cccactccaa gagagggttt tcttggtgaa gttcgtgcga tcccggagac ccaggacagc
     5701 gagctgcgct ctggctttcg tgaacatgtt gttgaggcta gagaggatct tgagaagagg
     5761 gccgcaccgg aatcctggac caggtgatga tgatgggcaa cgttcacgta gcagctcttc
     5821 tgctcaacta cggtgcagat tcgaactgcg aggaccccac taccttctcc cgcccggtgc
     5881 acgacgcagc gcgggaaggc ttcctggaca cgctggtggt gctgcacggg tcaggggctc
     5941 ggctggatgt gcgcgatgcc tggggtcgcc tgccgctcga cttggcccaa gagcggggac
     6001 atcaagacat cgtgcgatat ttgcgttccg ctgggtgctc tttgtgttcc gctgggtggt
     6061 ctttgtgtac cgctgggaac gtcgcccaga ccgacgggca tagatctttt tccctctgcc
     6121 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     6181 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     6241 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     6301 gcccatatgc tg
//
