LOCUS       62056_4745       12969 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..1819
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2744..2761
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2793..3045
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3067..3091
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4391..5017
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5454..6518
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6587..6781
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7125..7265
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7451..8039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8129..8920)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9345..9369
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9447..9621)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9947..10969)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10992..11351)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(11446..11547)
                     /label=TRP1 promoter
     intron          12841..12956
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
       61 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
      121 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
      181 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
      241 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
      301 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
      361 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
      421 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
      481 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
      541 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
      601 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
      661 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
      721 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
      781 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
      841 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
      901 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
      961 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
     1021 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
     1081 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
     1141 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
     1201 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
     1261 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
     1321 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
     1381 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
     1441 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
     1501 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
     1561 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
     1621 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
     1681 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
     1741 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
     1801 gcagcaggga ggcaaacaag ctggtggagg aggttccggc ggtggcggtt ccggtggcgg
     1861 aggttccatg cctcctatca tccattgcgt ccgccacgcc caggcaatcc acaatctctc
     1921 tgtcgcaaac cacgttatcc ccgatcccat cctcacagat ctgggcaacg aacaatgccg
     1981 caagctccgt gagaagtttc cttaccattc cgacgtggaa ttggtcgtct cctcgcccct
     2041 gcgtcgcacg atcgccacaa gcctccaggg cttcgagccc gtcttccagt cgcgggaggg
     2101 gctgaagttg atcgttcatc cggatctcca ggagacgagc gatgttccct gtgatacggg
     2161 gagtaatccg gaggttttga gggaggagat tgagaagggt gggcttccgg ttgatttggg
     2221 gttgctgttt gatgggtgga acagtaagaa aggaccgtat gcgcctacca acaaggagat
     2281 caagaatcga gcccgtgctg cccgtcggtg gctgaaggca cggccggaaa aggtgattgt
     2341 tgtcgttacc catggtggat tcttgcacta tttcaccgag gactgggagg atagcagtga
     2401 ataccagggg accggctggg ccaataccga attccgcacg ttcgagtttg ccgatgtcga
     2461 gcataaagat gatctggaag gctacgggtt ggacggtgac aacgctacgt tgatcgagac
     2521 ggttgaatct cgccgacgtc gtgggaagga tggcacaaca cccagccgtg agcagcagaa
     2581 ggttctgtac aagcttgggg ttcagggctg ggataaccag ggcctcgcgc ttagtgtagc
     2641 tgagagagag aagaccaagg tgcctcaagg cgaggaggca caaagcaagc agtcagcaat
     2701 gcgtaatctc gatgctggat tctccattga aaaagaagct agccaccacc accaccacca
     2761 cgtgtgaatt ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     2821 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2881 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2941 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3001 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
     3061 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     3121 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     3181 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     3241 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     3301 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     3361 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     3421 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3481 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3541 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3601 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3661 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3721 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3781 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3841 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3901 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3961 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     4021 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     4081 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     4141 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     4201 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     4261 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     4321 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     4381 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     4441 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4501 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4561 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4621 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4681 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4741 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4801 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4861 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4921 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4981 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     5041 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     5101 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     5161 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     5221 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     5281 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     5341 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     5401 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     5461 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     5521 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     5581 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     5641 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     5701 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     5761 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     5821 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     5881 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     5941 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     6001 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     6061 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     6121 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     6181 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     6241 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     6301 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     6361 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     6421 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     6481 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     6541 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     6601 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     6661 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     6721 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     6781 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     6841 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     6901 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     6961 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7021 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7081 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7141 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7201 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7261 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7861 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8101 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
     8161 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     8221 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     8281 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     8341 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     8401 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     8461 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     8521 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     8581 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     8641 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     8701 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     8761 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     8821 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     8881 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     8941 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     9001 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     9061 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
     9121 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
     9181 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
     9241 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
     9301 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
     9361 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
     9421 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg
     9481 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     9541 atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac
     9601 tcacacatta ttatggagaa actcgaggca ttgcagatga gctgtatctg gaagaggtaa
     9661 acccgaaacg cgttttattc ttgttgacat ggagctatta aatcactaga aggcactctt
     9721 tgctgcttgg acaaatgaac gtatcttatc gagatcctga acaccatttg tctcaactcc
     9781 ggagctgaca tcgacaccaa cgatcttata tccagattcg tcaagctgtt tgatgatttc
     9841 agtaacgtta agtggatcat gataatcatc gcaagaccgg caacaggatt caatcttaag
     9901 aaactttatt gccaaatgtt tgaacgatct gcaggtcgac ggactatttc tttgccctcg
     9961 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
    10021 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
    10081 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
    10141 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
    10201 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
    10261 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
    10321 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
    10381 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
    10441 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
    10501 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
    10561 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
    10621 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
    10681 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
    10741 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
    10801 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    10861 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    10921 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gttacttcct
    10981 aatcgaagct ttgcttgggt agaataggta agtcagattg aatctgaaat aaagggagga
    11041 agggcgaact taagaaggta tgaccgggtc gttcacttac cttgcttgac aaacgcacca
    11101 agttatcgtg caccaagcag cagatgataa taatgtcctc gttcctgtct gctaataaga
    11161 gtcacacttc gagcgccgcc gctactgcta caagtggggc tgatctgacc agttgcctaa
    11221 atgaaccatc ttgtcaaacg acacaaattt tgtgctcacc gcctggacga ctaaaccaaa
    11281 ataggcattc attgttgacc tccactagct ccagccaagc ccaaaaagtg ctccttcaat
    11341 atcatcttct gtcgagtcta gagcacattc tgcggcctct gtgctctgca agccgcaaac
    11401 tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt
    11461 tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc
    11521 ctctcctttt cttttttcga ccgaattgag ctcggtacca aacgttgcta gcagttccag
    11581 gtggaatgtt atgatgagca ttgtattaaa tcaggagata tagcatgatc tctagttagc
    11641 tcaccacaaa agtcagacgg cgtaaccaaa agtcacacaa cacaagctgt aaggatttcg
    11701 gcacggctac ggaagacgga gaagccacct tcagtggact cgagtaccat ttaattctat
    11761 ttgtgtttga tcgagaccta atacagcccc tacaacgacc atcaaagtcg tatagctacc
    11821 agtgaggaag tggactcaaa tcgacttcag caacatctcc tggataaact ttaagcctaa
    11881 actatacaga ataagatagg tggagagctt ataccgagct cccaaatctg tccagatcat
    11941 ggttgaccgg tgcctggatc ttcctataga atcatcctta ttcgttgacc tagctgattc
    12001 tggagtgacc cagagggtca tgacttgagc ctaaaatccg ccgcctccac catttgtaga
    12061 aaaatgtgac gaactcgtga gctctgtaca gtgaccggtg actctttctg gcatgcggag
    12121 agacggacgg acgcagagag aagggctgag taataagcca ctggccagac agctctggcg
    12181 gctctgaggt gcagtggatg attattaatc cgggaccggc cgcccctccg ccccgaagtg
    12241 gaaaggctgg tgtgcccctc gttgaccaag aatctattgc atcatcggag aatatggagc
    12301 ttcatcgaat caccggcagt aagcgaagga gaatgtgaag ccaggggtgt atagccgtcg
    12361 gcgaaatagc atgccattaa cctaggtaca gaagtccaat tgcttccgat ctggtaaaag
    12421 attcacgaga tagtaccttc tccgaagtag gtagagcgag tacccggcgc gtaagctccc
    12481 taattggccc atccggcatc tgtagggcgt ccaaatatcg tgcctctcct gctttgcccg
    12541 gtgtatgaaa ccggaaaggc cgctcaggag ctggccagcg gcgcagaccg ggaacacaag
    12601 ctggcagtcg acccatccgg tgctctgcac tcgacctgct gaggtccctc agtccctggt
    12661 aggcagcttt gccccgtctg tccgcccggt gtgtcggcgg ggttgacaag gtcgttgcgt
    12721 cagtccaaca tttgttgcca tattttcctg ctctccccac cagctgctct tttcttttct
    12781 ctttcttttc ccatcttcag tatattcatc ttcccatcca agaaccttta tttcccctaa
    12841 gtaagtactt tgctacatcc atactccatc cttcccatcc cttattcctt tgaacctttc
    12901 agttcgagct ttcccacttc atcgcagctt gactaacagc taccccgctt gagcagacat
    12961 caccatggt
//
