LOCUS       62056_4740       10959 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..727
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             734..751
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      783..1035
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1057..1081
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2381..3007
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3444..4508
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4577..4771
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5115..5255
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5441..6029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6119..6910)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7335..7359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7437..7611)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7937..8959)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8982..9341)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(9436..9537)
                     /label=TRP1 promoter
     intron          10831..10946
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
     3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     4021 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     4081 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     4141 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     4201 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     4261 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     4321 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     4381 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     4441 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     4501 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     4561 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     4621 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     4681 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     4741 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     4801 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     4861 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     4921 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     4981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     5161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     5221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     6121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     6181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     6241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     6301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     6361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     6421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     6481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     6541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     6601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     6661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     6721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     6781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     6841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     6901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     6961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     7021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     7081 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     7141 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     7201 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     7261 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     7321 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     7381 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
     7441 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     7501 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     7561 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgaggca
     7621 ttgcagatga gctgtatctg gaagaggtaa acccgaaacg cgttttattc ttgttgacat
     7681 ggagctatta aatcactaga aggcactctt tgctgcttgg acaaatgaac gtatcttatc
     7741 gagatcctga acaccatttg tctcaactcc ggagctgaca tcgacaccaa cgatcttata
     7801 tccagattcg tcaagctgtt tgatgatttc agtaacgtta agtggatcat gataatcatc
     7861 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     7921 gcaggtcgac ggactatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     7981 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     8041 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     8101 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     8161 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     8221 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     8281 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     8341 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     8401 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     8461 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     8521 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     8581 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
     8641 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     8701 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     8761 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     8821 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     8881 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     8941 tgagttcagg ctttttcatg gttacttcct aatcgaagct ttgcttgggt agaataggta
     9001 agtcagattg aatctgaaat aaagggagga agggcgaact taagaaggta tgaccgggtc
     9061 gttcacttac cttgcttgac aaacgcacca agttatcgtg caccaagcag cagatgataa
     9121 taatgtcctc gttcctgtct gctaataaga gtcacacttc gagcgccgcc gctactgcta
     9181 caagtggggc tgatctgacc agttgcctaa atgaaccatc ttgtcaaacg acacaaattt
     9241 tgtgctcacc gcctggacga ctaaaccaaa ataggcattc attgttgacc tccactagct
     9301 ccagccaagc ccaaaaagtg ctccttcaat atcatcttct gtcgagtcta gagcacattc
     9361 tgcggcctct gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa
     9421 attaataaca gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct
     9481 caatagtcac caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaattgag
     9541 ctcggtacca aacgttgcta gcagttccag gtggaatgtt atgatgagca ttgtattaaa
     9601 tcaggagata tagcatgatc tctagttagc tcaccacaaa agtcagacgg cgtaaccaaa
     9661 agtcacacaa cacaagctgt aaggatttcg gcacggctac ggaagacgga gaagccacct
     9721 tcagtggact cgagtaccat ttaattctat ttgtgtttga tcgagaccta atacagcccc
     9781 tacaacgacc atcaaagtcg tatagctacc agtgaggaag tggactcaaa tcgacttcag
     9841 caacatctcc tggataaact ttaagcctaa actatacaga ataagatagg tggagagctt
     9901 ataccgagct cccaaatctg tccagatcat ggttgaccgg tgcctggatc ttcctataga
     9961 atcatcctta ttcgttgacc tagctgattc tggagtgacc cagagggtca tgacttgagc
    10021 ctaaaatccg ccgcctccac catttgtaga aaaatgtgac gaactcgtga gctctgtaca
    10081 gtgaccggtg actctttctg gcatgcggag agacggacgg acgcagagag aagggctgag
    10141 taataagcca ctggccagac agctctggcg gctctgaggt gcagtggatg attattaatc
    10201 cgggaccggc cgcccctccg ccccgaagtg gaaaggctgg tgtgcccctc gttgaccaag
    10261 aatctattgc atcatcggag aatatggagc ttcatcgaat caccggcagt aagcgaagga
    10321 gaatgtgaag ccaggggtgt atagccgtcg gcgaaatagc atgccattaa cctaggtaca
    10381 gaagtccaat tgcttccgat ctggtaaaag attcacgaga tagtaccttc tccgaagtag
    10441 gtagagcgag tacccggcgc gtaagctccc taattggccc atccggcatc tgtagggcgt
    10501 ccaaatatcg tgcctctcct gctttgcccg gtgtatgaaa ccggaaaggc cgctcaggag
    10561 ctggccagcg gcgcagaccg ggaacacaag ctggcagtcg acccatccgg tgctctgcac
    10621 tcgacctgct gaggtccctc agtccctggt aggcagcttt gccccgtctg tccgcccggt
    10681 gtgtcggcgg ggttgacaag gtcgttgcgt cagtccaaca tttgttgcca tattttcctg
    10741 ctctccccac cagctgctct tttcttttct ctttcttttc ccatcttcag tatattcatc
    10801 ttcccatcca agaaccttta tttcccctaa gtaagtactt tgctacatcc atactccatc
    10861 cttcccatcc cttattcctt tgaacctttc agttcgagct ttcccacttc atcgcagctt
    10921 gactaacagc taccccgctt gagcagacat caccatggt
//
