LOCUS       62056_4735       10965 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..733
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             740..757
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      789..1041
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1063..1087
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2387..3013
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3450..4514
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4583..4777
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5121..5261
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5447..6035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6125..6916)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7341..7365
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7443..7617)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7943..8965)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8988..9347)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(9442..9543)
                     /label=TRP1 promoter
     intron          10837..10952
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtatgatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
       61 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      121 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      181 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      241 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      301 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      361 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      421 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      481 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      541 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      601 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      661 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      721 cgagctgtac aaggctagcc accaccacca ccaccacgtg tgaattggtg accagctcga
      781 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
      841 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
      901 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
      961 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1021 tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg
     1081 ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa
     1141 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     1201 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     1261 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     1321 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     1381 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     1441 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     1501 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     1561 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     1621 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     1681 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     1741 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     1801 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     1861 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     1921 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     1981 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     2041 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     2101 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     2161 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     2221 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     2281 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     2341 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     2401 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     2461 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     2521 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     2581 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     2641 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     2701 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     2761 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     2821 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     2881 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     2941 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     3001 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     3061 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     3121 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     3181 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     3241 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     3301 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     3361 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     3421 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     3481 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     3541 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     3601 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     3661 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     3721 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     3781 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     3841 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     3901 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     3961 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     4021 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     4081 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     4141 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     4201 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     4261 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     4321 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     4381 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     4441 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     4501 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     4561 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     4621 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     4681 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     4741 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     4801 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     4861 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     4921 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     4981 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5101 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     5161 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5221 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     5281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5881 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     6121 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     6181 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     6241 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     6301 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     6361 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     6421 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     6481 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     6541 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     6601 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     6661 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     6721 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     6781 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     6841 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     6901 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
     6961 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     7021 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     7081 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     7141 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     7201 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     7261 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     7321 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     7381 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
     7441 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
     7501 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
     7561 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
     7621 gaggcattgc agatgagctg tatctggaag aggtaaaccc gaaacgcgtt ttattcttgt
     7681 tgacatggag ctattaaatc actagaaggc actctttgct gcttggacaa atgaacgtat
     7741 cttatcgaga tcctgaacac catttgtctc aactccggag ctgacatcga caccaacgat
     7801 cttatatcca gattcgtcaa gctgtttgat gatttcagta acgttaagtg gatcatgata
     7861 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     7921 cgatctgcag gtcgacggac tatttctttg ccctcggacg agtgctgggg cgtcggtttc
     7981 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     8041 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     8101 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     8161 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga
     8221 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     8281 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     8341 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     8401 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     8461 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     8521 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     8581 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg
     8641 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     8701 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg
     8761 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     8821 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     8881 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     8941 tcgcggtgag ttcaggcttt ttcatggtta cttcctaatc gaagctttgc ttgggtagaa
     9001 taggtaagtc agattgaatc tgaaataaag ggaggaaggg cgaacttaag aaggtatgac
     9061 cgggtcgttc acttaccttg cttgacaaac gcaccaagtt atcgtgcacc aagcagcaga
     9121 tgataataat gtcctcgttc ctgtctgcta ataagagtca cacttcgagc gccgccgcta
     9181 ctgctacaag tggggctgat ctgaccagtt gcctaaatga accatcttgt caaacgacac
     9241 aaattttgtg ctcaccgcct ggacgactaa accaaaatag gcattcattg ttgacctcca
     9301 ctagctccag ccaagcccaa aaagtgctcc ttcaatatca tcttctgtcg agtctagagc
     9361 acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc
     9421 tgtgaaatta ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca
     9481 cgtgctcaat agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga
     9541 attgagctcg gtaccaaacg ttgctagcag ttccaggtgg aatgttatga tgagcattgt
     9601 attaaatcag gagatatagc atgatctcta gttagctcac cacaaaagtc agacggcgta
     9661 accaaaagtc acacaacaca agctgtaagg atttcggcac ggctacggaa gacggagaag
     9721 ccaccttcag tggactcgag taccatttaa ttctatttgt gtttgatcga gacctaatac
     9781 agcccctaca acgaccatca aagtcgtata gctaccagtg aggaagtgga ctcaaatcga
     9841 cttcagcaac atctcctgga taaactttaa gcctaaacta tacagaataa gataggtgga
     9901 gagcttatac cgagctccca aatctgtcca gatcatggtt gaccggtgcc tggatcttcc
     9961 tatagaatca tccttattcg ttgacctagc tgattctgga gtgacccaga gggtcatgac
    10021 ttgagcctaa aatccgccgc ctccaccatt tgtagaaaaa tgtgacgaac tcgtgagctc
    10081 tgtacagtga ccggtgactc tttctggcat gcggagagac ggacggacgc agagagaagg
    10141 gctgagtaat aagccactgg ccagacagct ctggcggctc tgaggtgcag tggatgatta
    10201 ttaatccggg accggccgcc cctccgcccc gaagtggaaa ggctggtgtg cccctcgttg
    10261 accaagaatc tattgcatca tcggagaata tggagcttca tcgaatcacc ggcagtaagc
    10321 gaaggagaat gtgaagccag gggtgtatag ccgtcggcga aatagcatgc cattaaccta
    10381 ggtacagaag tccaattgct tccgatctgg taaaagattc acgagatagt accttctccg
    10441 aagtaggtag agcgagtacc cggcgcgtaa gctccctaat tggcccatcc ggcatctgta
    10501 gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt atgaaaccgg aaaggccgct
    10561 caggagctgg ccagcggcgc agaccgggaa cacaagctgg cagtcgaccc atccggtgct
    10621 ctgcactcga cctgctgagg tccctcagtc cctggtaggc agctttgccc cgtctgtccg
    10681 cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt ccaacatttg ttgccatatt
    10741 ttcctgctct ccccaccagc tgctcttttc ttttctcttt cttttcccat cttcagtata
    10801 ttcatcttcc catccaagaa cctttatttc ccctaagtaa gtactttgct acatccatac
    10861 tccatccttc ccatccctta ttcctttgaa cctttcagtt cgagctttcc cacttcatcg
    10921 cagcttgact aacagctacc ccgcttgagc agacatcacc atggt
//
