LOCUS       62056_4765       13662 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..1819
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             3437..3454
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      3486..3738
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3760..3784
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5084..5710
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6147..7211
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7280..7474
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7818..7958
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8822..9613)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10038..10062
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10140..10314)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10640..11662)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11685..12044)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     promoter        complement(12139..12240)
                     /label=TRP1 promoter
     intron          13534..13649
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
ORIGIN
        1 agatctgact agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
       61 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
      121 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
      181 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
      241 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
      301 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
      361 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
      421 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
      481 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
      541 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
      601 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
      661 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
      721 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
      781 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
      841 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
      901 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
      961 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
     1021 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
     1081 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
     1141 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
     1201 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
     1261 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
     1321 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
     1381 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
     1441 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
     1501 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
     1561 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
     1621 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
     1681 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
     1741 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
     1801 gcagcaggga ggcaaacaag ctggtggagg aggttccggc ggtggcggtt ccggtggcgg
     1861 aggttccatg aaggaagaaa ttgacgcccc agtttccacc gacgcttctg gcacagattt
     1921 agagaatgct cgagatcagc catctggcga gaagcccact gccgtggata tttacttggt
     1981 ggaatgggat gggcctaacg acccagaact gcctatgaac tttcccctat ggaagaaatc
     2041 gctcatcact tgcatcttca gcacattgac catctgggta accttctcca gcagcgtctt
     2101 cagtgctgct gctgggatca cttccaaaga gtttcatgtt tcagttgagg tcatgacgtt
     2161 gggaaccagt ttgaccgtcc tgggctttac ggtgggaccg ctggtctggg ggcccatgtc
     2221 tgagttatat ggccgactga agccgctcta cattgggtat gccatcttta tcatcttcca
     2281 agttcctgtg gcggttgccc agaacctcga gaccttgatg cttgcccgtt tctttctggg
     2341 tttcttcggc acctccgccc tcgcaatcat tcccggtgct ctggcggact tttggggtcc
     2401 agtggagcgc gcgattgcca tctctttatt ttccgcggct accttcgttg ggccgatatt
     2461 tggaccgatc atgtacgtga atacaatgat aacattggta aatggggtct ggacgcgtgc
     2521 taatcacgac atgagcagtg ggggcttcat cgtcgactca tctctgggat ggcggtggac
     2581 tgcctggatc actatgatcc cagcttcatt cttcggcata atcgccttcc tcacccttcc
     2641 cgagacatac cacccggtcc tcctccagcg ccgtgccagc cgcctccgta aagagacgcg
     2701 tatctgggcc tatcactccc gcctggatga gaatacaccg acgtttggtg aaatcctgac
     2761 gaaatatctc ttccgtccaa ttcagatgct tttcttggag ccgatcctcg tctgcatgac
     2821 aatctatatc tcccttatct atggaatctt atacctcttc ttcgtggcat acccaattgc
     2881 tttccgcgag gtccggaatt ggaagtcctt gggaattgcg gccttacctt tcctagggat
     2941 cctagtcggg gtccttatgg gctgcctcct ggtcaccatc gcgacacgcc tgtggtacgc
     3001 tcccaagcta cagaatgggt ccgtcgtacc ggaagatcgt cttcccccca tgattgttgc
     3061 ggctattctc ctgccaatcg ggctattctg gtttggatgg acatcgagcc cgagtatttc
     3121 gtgggcgcca caagccattg caggggcgcc tatcggaatg gggattttga tgatctggat
     3181 gcaaggcctg aactatctga ttgacgtata tctggtggtt gctaattcgg ccatgtcggc
     3241 caacactctc attcgcagcg cggtaagtgc tgcgttccct ctctttgcga cggctatgta
     3301 ccataagttg ggagtggact gggccacgtc cctgctcggc tttctttcta ttgcgatgat
     3361 cccgatcccc gttatcttct atttctatgg ggcgaagatt cgggctttga gtcgctttag
     3421 tccaaaatgg gctagccacc accaccacca ccacgtgtga attggtgacc agctcgaatt
     3481 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     3541 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     3601 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     3661 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     3721 catctatgtt actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt
     3781 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
     3841 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     3901 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     3961 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     4021 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     4081 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     4141 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     4201 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     4261 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     4321 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     4381 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     4441 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     4501 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     4561 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     4621 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     4681 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     4741 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     4801 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     4861 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     4921 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     4981 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     5041 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     5101 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     5161 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     5221 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     5281 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     5341 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     5401 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     5461 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     5521 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     5581 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     5641 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     5701 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     5761 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     5821 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     5881 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     5941 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     6001 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     6061 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     6121 ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg
     6181 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     6241 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     6301 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     6361 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     6421 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     6481 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     6541 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     6601 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     6661 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     6721 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     6781 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     6841 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     6901 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     6961 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     7021 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     7081 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     7141 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     7201 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     7261 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     7321 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     7381 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     7441 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     7501 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     7561 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     7621 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     7681 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7741 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7801 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     7861 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7921 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
     8821 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
     8881 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
     8941 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
     9001 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
     9061 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
     9121 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
     9181 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
     9241 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
     9301 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
     9361 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
     9421 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
     9481 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
     9541 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
     9601 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
     9661 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
     9721 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
     9781 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
     9841 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
     9901 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
     9961 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    10021 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    10081 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
    10141 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
    10201 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
    10261 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
    10321 gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcgtttta ttcttgttga
    10381 catggagcta ttaaatcact agaaggcact ctttgctgct tggacaaatg aacgtatctt
    10441 atcgagatcc tgaacaccat ttgtctcaac tccggagctg acatcgacac caacgatctt
    10501 atatccagat tcgtcaagct gtttgatgat ttcagtaacg ttaagtggat catgataatc
    10561 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
    10621 tctgcaggtc gacggactat ttctttgccc tcggacgagt gctggggcgt cggtttccac
    10681 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
    10741 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
    10801 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
    10861 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
    10921 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
    10981 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
    11041 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
    11101 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
    11161 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
    11221 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
    11281 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
    11341 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
    11401 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
    11461 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
    11521 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
    11581 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
    11641 cggtgagttc aggctttttc atggttactt cctaatcgaa gctttgcttg ggtagaatag
    11701 gtaagtcaga ttgaatctga aataaaggga ggaagggcga acttaagaag gtatgaccgg
    11761 gtcgttcact taccttgctt gacaaacgca ccaagttatc gtgcaccaag cagcagatga
    11821 taataatgtc ctcgttcctg tctgctaata agagtcacac ttcgagcgcc gccgctactg
    11881 ctacaagtgg ggctgatctg accagttgcc taaatgaacc atcttgtcaa acgacacaaa
    11941 ttttgtgctc accgcctgga cgactaaacc aaaataggca ttcattgttg acctccacta
    12001 gctccagcca agcccaaaaa gtgctccttc aatatcatct tctgtcgagt ctagagcaca
    12061 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt
    12121 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt
    12181 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt
    12241 gagctcggta ccaaacgttg ctagcagttc caggtggaat gttatgatga gcattgtatt
    12301 aaatcaggag atatagcatg atctctagtt agctcaccac aaaagtcaga cggcgtaacc
    12361 aaaagtcaca caacacaagc tgtaaggatt tcggcacggc tacggaagac ggagaagcca
    12421 ccttcagtgg actcgagtac catttaattc tatttgtgtt tgatcgagac ctaatacagc
    12481 ccctacaacg accatcaaag tcgtatagct accagtgagg aagtggactc aaatcgactt
    12541 cagcaacatc tcctggataa actttaagcc taaactatac agaataagat aggtggagag
    12601 cttataccga gctcccaaat ctgtccagat catggttgac cggtgcctgg atcttcctat
    12661 agaatcatcc ttattcgttg acctagctga ttctggagtg acccagaggg tcatgacttg
    12721 agcctaaaat ccgccgcctc caccatttgt agaaaaatgt gacgaactcg tgagctctgt
    12781 acagtgaccg gtgactcttt ctggcatgcg gagagacgga cggacgcaga gagaagggct
    12841 gagtaataag ccactggcca gacagctctg gcggctctga ggtgcagtgg atgattatta
    12901 atccgggacc ggccgcccct ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc
    12961 aagaatctat tgcatcatcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa
    13021 ggagaatgtg aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt
    13081 acagaagtcc aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag
    13141 taggtagagc gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg
    13201 cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag
    13261 gagctggcca gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg
    13321 cactcgacct gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc
    13381 ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc
    13441 ctgctctccc caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc
    13501 atcttcccat ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc
    13561 atccttccca tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag
    13621 cttgactaac agctaccccg cttgagcaga catcaccatg gt
//
